Gazi ÇAPAR, Hayriye ŞENTÜRK ÇİFTÇİ, Sacide PEHLİVAN

Role of CYP3A4*1B gene variant in substance use disorder

Background and objective: This study aims to find out the possible association between the CYP3A4*1B gene variant (rs2740574) and substance use disorder susceptibility in the Turkish population. Materials and methods: 158 patients with substance use disorder and 100 healthy individuals matched for gender, age, and ethnicity were enrolled in the study. Genotyping was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using the MboII restriction endonuclease. The association between the CYP3A4*1B variant and substance use disorder was analyzed by using SPSS 21 and de-Finetti program. Results: CYP3A4 gene (MboII gene variant in 5’ promoter region) genotype distributions of substance use disorder patients were not significantly different from healthy controls. When the substance use and control groups were compared in terms of allele frequency, increased G allele frequency was observed in CYP3A4 variants in the substance use group (p: 0,042). Conclusion: This is the first study that investigates the association between the MboII gene variant in CYP3A4 gene 5’promoter region and substance use disorder in the literature. It was demonstrated that an increased G allele existed in Turkish substance use disorder patients. It also was planned to research the other variants of the CYP3A4 gene in the future.

Anahtar Kelimeler:

sustance use disorder, CYP3A4*1B, variant, DNA

Role of CYP3A4*1B Gene Variant In Substance Use Disorder

Objective: This study aims to find out the possible association between the CYP3A4*1B gene variant (rs2740574) and substance use disorder susceptibility in the Turkish population. Materials and Methods: 158 patients with substance use disorder and 100 healthy individuals matched for gender, age, and ethnicity were enrolled in the study. Genotyping was analyzed with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using the MboII restriction endonuclease. The association between the CYP3A4*1B variant and substance use disorder was analyzed using SPSS 21 and de-Finetti program. Results: The CYP3A4 gene (MboII gene variant in 5’ promoter region) genotype distributions of substance use disorder patients were not significantly different from the healthy controls. When the substance use and control groups were compared in terms of allele frequency, increased G allele frequency was observed in CYP3A4 variants in the substance use group (p: 0,042). Conclusion: This is the first study that investigates the association between the MboII gene variant in CYP3A4 gene 5’promoter region and substance use disorder in the literature. It was demonstrated that an increased G allele existed in Turkish substance use disorder patients. Plans have been made to research the other variants of the CYP3A4 gene in the future.

Keywords:

CYP3A4 substance abuse, MboII endonuclease, drug metabolism, addiction,

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