Mehmet BERKER, Emine UTLU ÖZEN, Fatma ÖZ BAĞCI

MEZENKİMAL KÖK HÜCRELERİN FARKLI ÇÖZELTİ VE SICAKLIKLARDA SAKLANMASI

Amaç: Mezenkimal Kök Hücreler (MKH) kendi kendini yenileme ve çeşitli hücre tiplerine farklılaşma yeteneğine sahiptir. Rejeneratif tıpta kullanımları günümüzde yaygın hale gelmiştir. Bununla birlikte, başarılı bir klinik uygulama için, MKH’lerin canlılığının ve potentliğinin transplantasyon öncesi hazırlık ve nakliye sırasında korunması gerekmektedir. Bu çalışmanın amacı; yeterince araştırılmamış olan bu konuda klinik olarak amaçlanan MKH etkinliğini korumak için önemli olan saklama koşulları ile ilgili literatüre katkı sağlamaktır. Gereç ve Yöntem: 4°C’de ve oda sıcaklığında (24°C), Phosphate Buffered Saline (PBS) ve Serum Fizyolojik(SF) solüsyonunda kısa süreli in vitro depolamada MKH’lerin canlılığı ve CD73, CD90, CD105, CD19 yüzey antijenleri değerlendirildi. Sonuç: 4°C ve 24°C’de PBS ve SF solüsyonunda canlılık oranları ve yüzey antijenleri açısından sonuçlar benzer olsa da, PBS’te ve 4°C’de daha yüksek oranlar elde edilmiştir. Farklı ortam şartlarında MKH’lerin farklılaşma, yaşlanma ve çoğalma kapasiteleri analiz edilerek ileri araştırmalar yapılabilir.

Anahtar Kelimeler:

Mezenkimal Kök hücre, Flow Sitometri, Canlılık

STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES

Objective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparation and transportation. The aim of this study (an area which has not been adequately researched), is to contribute to the literature on storage conditions, which is important for maintaining the effectiveness of MSCs. Material and Method: The viability of MSCs and CD73, CD90, CD105, CD19 surface antigens were evaluated in short-term in vitro storage in Phosphate Buffered Saline (PBS) and Serum Physiological solution at 4°C and room temperature (24°C). Conclusion: Although MSCs are similar in terms of viability and surface antigens in PBS and SF solution at 4°C and 24°C, higher results were obtained in cells kept in PBS at 4°C. Further research can be conducted by analyzing the differentiation, aging and proliferation capacities of MSCs under different environmental conditions.

Keywords:

Mesenchymal Stem Cell, Flow Cytometry, Vitality,

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