Mehmet Yavuz PAKSOY, Perihan GÜRBÜZ, Gökçe Şeker KARATOPRAK, Müberra KOŞAR, Şengül Dilem DOĞAN, Duygu EROĞLU

TANACETUM ZAHLBRUCKNERI (NÁB.) GRIERSON' NUN SEKONDER METABOLİTLERİ ve ANTİOKSİDANETKİLERİ*

Bu çalışma kapsamında Asteraceae familyasına dahilendemik bir tür olan Tanacetum zahlbruckneri (Náb.)Grierson bitkisinin toprak üstü kısımlarından hazırlananmetanol ekstresinin DPPH?, ABTS?+süpürücü aktivitesi ve ?-karoten-linoleik asitle oksidasyon yöntemi ile antioksidan aktiviteleri, Folin- Ciocalteuyöntemiyle toplam fenol, flavonit ve flavonol içeriğiincelenmiştir. Metanol ekstresinden sıvı-sıvı partisyonyöntemiyle n-hekzan, dietil eter, diklorometan ve nbutanol ekstreleri hazırlanmış, n-butanol ekstresindençeşitli kromatografik yöntemler kullanılarak, klorojenikasit (1), rutin (2) ve konduritol F (3) izole edilmiştir.Elde edilen bileşiklerin yapıları spektroskopik yöntemler (IR,1H-NMR,13C-NMR, 2D-NMR, IR, MS) kullanılarakaydınlatılmıştır. Bu çalışma ile Konduritol F Tanacetumtürlerinden ilk defa izole edilmiştir. Klorojenik asit verutinin bitkinin metanol ekstresindeki kantitatif tayiniYüksek Basınçlı Sıvı Kromatografisi ile gerçekleştirilmiştir. İzole edilen bileşiklerin antioksidan aktiviteleri ölçülmüş ve klorojenik asitin aktivitesi, rutin vemetanol ekstresinden daha yüksek bulunmuştur.

SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITIES OF TANACETUM ZAHLBRUCKNERI (NÁB.) GRIERSON

In the current study, antioxidant activity of methanolextract prepared from Tanacetum zahlbruckneri(Asteraceae) was studied with DPPH?, ABTS+radicalscavenging and beta-carotene-linoleic acid cooxidationassays. Total phenol, flavonoid and flavonol contents ofextracts were determined with Folin- Ciocalteu method.n-Hexane, diethyl ether, dichloromethane and n-butanolextracts were prepared from methanol extract withsolvent partitioning and chlorogenic acid, rutin andconduritol F were isolated from n-BuOH extract withvarious chromatographic methods. Structures of thecompounds were elucidated by extensive 1D- and 2DNMR spectroscopic analysis in combination with IR, MSexperiments. Conduritol F was isolated for the first timefrom Tanacetum species. Quantitative analyses ofchlorogenic acid and rutin in MEOH extract were determined with High Pressure Liquid Chromatography(HPLC). Antioxidant activities of isolated compoundswere studied and chlorogenic acid was found to bemore active than rutin and methanol extract.

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