Eren DEMİRPOLAT, Mükerrem Betül YERER AYCAN

6-SHOGAOL, 6-GİNGEROL VE KURKUMİN’ İN A549 HÜCRELERİNDE CANLILIK ÜZERİNE ETKİLERİ

Çalışmamızda A549 küçük hücreli olmayan akciğer kanseri hücrelerinde Zingiber officinale’de bulunan 6- gingerol ile 6-shogaolün antikanser etkileri canlılık üzerinden araştırılmıştır. Deneyler hem IL-1β’ ya maruz kalan hem de maruz kalmayan hücrelerde yürütüldü. Curcuma longa’ da bulunan kurkumin bilinen doğal bir antikanser molekül olduğundan çalışmada pozitif kontrol olarak kullanıldı ve kurkumine yapıca benzer olan 6 -gingerol ile 6-shogaolün canlılık üzerine olan etkileri kurkuminle kıyaslandı. MTT (3-(4,5-dimetiltiazol-2-il)- 2,5-difenil tetrazolyum bromür) deneyleri öncelikle 6- gingerol ve 6-shogaolün birbirini logaritmik şekilde seyreden konsantrasyonlarında yapıldı. Daha sonra etki görülen konsantrasyon aralığını kapsayacak şekilde IC50 değerini belirlemek için deneyler tekrarlandı. Sonuç olarak 6-shogaolun 24 sa IC50 değeri IL-β’ ya maruz kalmayan hücrelerde 62,5 μM; IL-β’ ya maruz kalan hücrelerde ise 63,2 μM olarak bulundu. Kurkumin 100 μM konsantrasyonu ile kıyaslandığında 6-shogaolün daha önceden antikanser özelliği gösterilmiş olan kurkumin kadar etkin olduğu görülürken; 6-gingerol için canlılığı azaltıcı yönde bir bulguya rastlanmadı. 6-shogaol’ un canlılığı azaltıcı etkisinin altında yatan moleküler mekanizmalar araştırılmaya değerdir.

Anahtar Kelimeler:

A549, 6-gingerol, 6-shogaol, kurkumin, IL-1β

Effects of 6-Shogaol, 6-Gingerol and Curcumin on Cell Viability in A549 Cancer Cell Line

In our study we investigated anticancer effects of 6- gingerol and 6-shogaol from Zingiber officinale. Anticancer effect was monitored by using cell viability assay. Experiments are performed on both A549 cells that are incubated with IL-1β (interleukin 1 β) and cells which are not incubated with it. Curcumin from Curcuma longa, a natural compound that is known for its anticancer effect, is used as a positive control and effects of 6- gingerol and 6-shogaol are compared with it. MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed for wide range concentrations. Concentration changes were 10 fold. To obtain IC50 values of 6-shogol and 6-gingerol, MTT assays were performed again, using the previous concentrations but in a narrower range.According to the assays 6-shogaol’s 24 h IC50 determined as 62,5 μM for A549 cells which were not incubated with IL-1β and 63,2 μM for the cells which were exposed to IL-1β. 6-shogaol’s cytotoxic effect was as much as curcumin which is a known natural anticancer molecule, while 6-gingerol did not show any effect towards decreasing the cell viability. Mechanisms which underly 6-shogoal’s cytotoxic effect are worth for further investigation.

Keywords:

A549, 6-gingerol, 6-shogaol, curcumin, IL- 1β,

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