Nazmi ÇETİN, Dinç EŞSİZ, Hakan SALTAŞ, Gökhan ERASLAN

Effects of chronic lead intake via drinking water on some hematologic parameters in mice

Bu çalışma, 250 ve 1000 ppm dozunda kurşun asetat içeren içme sularının farelere kronik uygulanmasının bazı hematolojik parametrelerelere etkisini incelemek amacıyla yapılmıştır. Çalışmada 6 aylık, 35-40 g ağırlığında 120 erkek beyaz fare kullanıldı. Hayvanlar her bir grupta 40 fare olacak şekilde üç eşit gruba ayrıldı. I.grup control grubu olarak tutulurken II. ve III. gruptaki farelere 250 ve 1000 ppm kurşun asetat içeren su 120 gün süreyle verildi. Hayvanlardan 60., 90. ve 120. günlerde kalpten kan örnekleri alınarak incelendi. İncelenen hematolojik parametrelerde 60. ve 90. günlerde her hangi bir farklılık belirlenemedi. 1000 ppm dozda kurşun alan III. gruptaki hayvanların kontrol gurubuna göre eritrosit, lökosit, platelet sayısı ve lenfosit oranı ile ile hemoglobin, hematokrit, MCV ve MCH değerlerinde önemli bir azalma gözlenirken nötrofil oranında ise önemli bir artma tespit edilmiştir. Sonuç olarak, 250 ppm dozda kronik olarak kurşun alımının hematalojik parametreleri etkilemediği buna karşılık 1000 ppm dozda kurşun asetatın alımının ise hematolojik bozukluklara yol açtığı gözlenmiştir.

Farelerde içme suyu ile kronik kurşun alımının bazı hematolojik parametreler üzerine etkisi

The aim of this study was to investigate the effects of chronic lead intake by drinking water at the dosages of 250 and 1000 ppm of lead (as lead acetate) on some hematologic parameter in albino mice. A total of 120 six-month-old 120 white male albino mice, weighing 35- 40 g fed with same pellet feed and water were used in this study. Animals were equally divided into three groups of 40 animals each. Group I served as a control. Group II and group III received 250 ppm and 1000 ppm lead acetate via drinking water during 120 days, respectively. Blood samples were taken by cardiac puncture on days 60, 90 and 120 of study from animals. On days 60 and 90 there were no statistical difference in hematological parameters between groups. On day 120, mice in group III received 1000 ppm lead ecetate had significantly lower erythrocyte count, hematocrit and hemoglobin, MCV and MCH values than the control mice. Additionally, platelet counts, total leukocyte counts and lymphocyte ratios were significantly lower in lead-treated mice than the control animals. On the other hand, neutrophil ratio in lead treated mice were significantly elevated compared with the control mice. In conclusion, it was observed that lead acetate at the dose of 250 ppm does not affect the hematologic parameters while lead acetate at the dose of 1000 ppm leads to hematological disorders.

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