DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Bu çalısmada, deneysel ketozis oluşturulan koyunlarda % 20’lik total parenteral lipit emülsiyonları’nın (TPLE) % 30 Dekstroz tedavisine karşı etkinliği araştırıldı. Onsekiz adet gebe olmayan ve laktasyon dönemi dışındaki koyunlar her grupta 6 adet koyun olacak şekilde üç gruba ayrıldı. Bütün gruplarda deneysel ketozis oluşturuldu. Bu amaçla koyunların herbirine üç gün boyunca derialtı phlorhizin enjekte edildi (100 mg/kg) ve sınırlı yem (toplam rasyonun ¼’ü) verildi. Tüm gruplarda ketozise ait klinik belirtiler oluşturulduktan sonra birinci gruba kontrol amacıyla % 0,9 NaCl, ikinci gruba standart tedavi amacıyla % 30 Dekstroz ve üçüncü gruba % 20'lik TPLE damar içi yola uygulandı. Dekstroz uygulaması sonrasında NEFA düzeyleri önemli oranda azaldı. Lipit emülsiyonu verilenlerde ise NEFA düzeylerindeki artış istatistikî açıdan önemli bulunmadı (p=0.05). Bu grupta trigliserid ve glukoz düzeyleri TPLE uygulamasını takiben diğer gruplara oranla yüksek bulundu. Koyunlarda ketozise ait klinik belirtiler yalnız lipit emülsiyonu verildikten sonra belirgin olarak düzeldi. Sonuç olarak deneysel açlık ketozisi oluşturulan koyunlarda % 30’luk Dekstrozun NEFA düzeylerini azaltmasına rağmen, % 20 TPLE’nin glikoz ve trigliserid düzeylerinde artış sağlayıp dekstroz uygulamalarına göre enerji açığını kapattığı belirlendi. Bu nedenle TPLE’nın, açlık ketozisinin klinik belirtilerinin düzeltilmesinde etkili olabileceği değerlendirildi.

Anahtar Kelimeler:

Ketozis, Koyun, Yağ emülsiyonu

The Effects of Treatment With Total Parenteral Lipid Emultion in Sheep Experimentally Induced Starvation Ketosis

In this study, the effects of Total Parenteral Lipid Emulsions (TPLE) and 30% Dekstroz was compared to treatment of experimentally induced starvation ketosis in sheep. Eighteen nonpregnant, nonlactating sheep were randomly divided into 3 different study groups each containing six sheep. Subcutan phloryzin (100 mg/ kg) was injected together with feed restriction (1/4 of total ration) to induce the ketosis and lipid mobilisation. After clinical symptoms for ketosis in all groups, for control 0,9% NaCl in first group, 30% Dekstroz in second group IV and 20% TPLE in third group IV were administered to the each sheep in groups. Mean NEFA levels was a tendency to decrease after dextrose administration in group administered dextrose. In TPLE group, mean NEFA levels were not siginificantly higher after TPLE administraion (p=0.05). Mean triglyceride and glucose levels in sheep given TPLE were significantly higher than those of other groups. Clinical signs was recovered after in group given TPLE only. In conclusion, although 30% dextroz treatment was the most effective in decreasing of NEFA level, 20% TPLE inhibited clinical signs of starvation ketozis in sheep by providing urgent energy demand with increase of trigliserid and glucose levels. For this reason, TPLE was evaluated to be effective in correcting the clinical symptoms of fasting ketosis.

Keywords:

Fat Emulsion, Ketosis, Sheep,

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