Mehmet Alparslan ÜNAL, Esra BALCIOĞLU, Pınar BİLGİCİ, Saim ÖZDAMAR

ADRİAMİSİNİN OLUŞTURDUĞU TESTİS HASARI ÜZERİNE E VİTAMİNİ VE SELENYUM’UN KORUYUCU ETKİSİNİN İNCELENMESİ

Adriamisin, kanser tedavisinde yaygın olarak kullanılan bir kemoterapi ilacıdır. Adriamisin kanser hücreleri yanında testis dahil birçok dokuda zararlı etki göstermektedir. Selenyum ve Vitamin E üreme organları ve kısırlık üzerine koruyucu özelliklere sahiptirler. Bu çalışmanın amacı, Adriamisin ile indüklenen testis hasarına karşı E vitamini ve Selenyumun koruyucu etkisini araştırmaktır. Çalışmada 64 erkek sıçan her grupta 8 adet olacak şekilde; Kontrol, Adriamisin, Vitamin E, Selenyum, Vitamin E+Selenyum, Adriamisin+Vitamin E, Adriamisin+Selenyum, Adriamisin+VitaminE+Selenyum gruplarına ayrıldı. Testis dokuları eksize edildi ve %10’lukformaldehit içinde fikse edilerek rutin histolojik doku takibi basamaklarından geçirilerek parafin bloklara gömüldü. 5µm kalınlığındaki kesitler alınarak histopatolojik değerlendirme için Hematoksilen&Eozin ile boyanarak ışık mikroskobunda incelendi. Seminifer tübüllerdeki hasar JTBS ile belirlendi. Biyokimyasal yöntemlerle, Malondealdehit, Süperoksit Dismutaz, Katalaz ve Glutatyon Peroksidaz aktivitelerine bakıldı. Daha sonra kandaki serum örneklerinden testesteron seviyeleri ölçümlendi. TUNEL yöntemiyle apoptotik hücrelerin sayımı yapıldı. Sonuç olarak, dokular histopatolojik olarak değerlendirildiğinde diğer gruplardan farklı olarak ADR grubuna ait testis dokusunda düzensizlikler gözlendi. TUNEL yöntemi sonuçlarına göre ADR grubunda TUNEL pozitif hücre sayısı diğer gruplarla kıyaslandığında artış tespit edilmiştir (p=0.001). Testis dokusundaki MDA (p=0.593), CAT (p=0.469), GPx (p=0.655) ve SOD (p=0.907) değerleri kıyaslandığında ise gruplar arasında anlamlı bir farklılık bulunmamıştır. Testosteron düzeyleri, ADR uygulanan tüm gruplarda daha düşük bulunmuştur. Bu bağlamda gruplar arasında testosteron düzeyleri açısından anlamlı fark ortaya çıkmıştır (p=0.001). Adriamisin uygulanması sıçan testis dokularında hasar oluşturmuştur. Vitamin E ve Selenyum tedavisinin testis dokularında kısmen düzeltici etkiye sahip olduğunu kanıtlamıştır.

Anahtar Kelimeler:

Adriamisin, Selenyum, Sıçan, Testis, Vitamin E

INVESTIGATION OF PROTECTIVE EFFECTS OF SELENIUM AND VITAMIN E ON ADRIAMYCIN-INDUCED TESTICULAR INJURY

Adriamycin is a widely used chemotherapy drug in the treatment of cancer. Adriamycin has harmful effects on many tissues including testis as well as cancercells. Selenium and Vitamin E have reproductive organs and protective properties on infertility. The aim of this study to investigate the protective effect of Vitamin E and Selenium against Adriamycinin duced testicular injury. In this study, 64 male rats were 8 in each group; Control, Adriamycin, Vitamin E, Selenium, Vitamin E+Selenium, Adriamycin+Vitamin E, Adriamycin+Selenium, Adriamycin+VitaminE+Selenium divided in to groups. The testis tissues were excised and fixed in 10% formaldehyde and embedded in parafin blocks through routine histological follow-up steps. Sections of 5 µm thickness were stained with Hematoxylen&Eosin for histopathological evaluation and studied underlight microscope. The damage in the seminiferous tubules was determined through JTBS. Malondealdehyde, Superoxide Dismutase, Catalase and Glutathione Peroxidase activities were investigated by biochemical methods. Testosterone levels were measured from the serum samples. Apoptotic cells were counted by the TUNEL method. As a result, when the histopathological evaluation of the tissues was completed, irregularities were observed in the testicular tissues of the ADR group, unlike the other groups. According to the results of the TUNEL method, the number of TUNEL positive cells in the ADR group was increased compared to the other groups (p=0.001). When the values of MDA (p = 0.593), CAT (p = 0.469), GPX (p = 0.655) and SOD (p = 0.907) were compared in testicular tissue, nosignificant difference was found between the groups. However, testosterone levels were lower in all ADR groups compared to the other groups and this decrease was statistically significant. Testosterone levels were found to be lower in all ADR groups. In this context, there was a significant difference between the groups in terms of testosterone levels (p = 0.001). As a result, Adriamycin administration caused damage to rat testicular tissue. Vitamin E and Selenium treatment have proven to have a partial corrective effect on testicular tissues.

Keywords:

Adriamycin, Rat, Selenıum, Testes, Vitamin E,

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