İnsan Kolon Kanseri Hücrelerine Karşı İnorganik Nanopartikül-Temelli İlaç Taşıyıcı Sistemlerin Kullanılması: Partikül Büyüklüğünün Antikanser Aktivitesine Etkisi

Günümüz nanopartikül teknolojisi, özellikle kanser nanotıp uygulamalarında kullanılmak üzere istenilen boyut, şekil ve malzemeye sahip nanopartikül-temelli ilaç taşıyıcı sistemlerinin sentezine olanak sağlamaktadır. Dolayısıyla, partikül boyutlarının antikanser aktivite üzerindeki etkisini anlamak, kanser tedavisinde yeni ilaç taşıyıcı sistemlerin geliştirilmesine katkıda bulunacaktır. Bu nedenle, bu çalışmada, ilaç taşıyıcı sistemler olarak iki farklı büyüklükteki inorganik temelli nanopartiküller (~ 55 ve 314 nm) kullanıldı ve boyutlarının hücresel birikim, sitotoksisite ve apoptoz üzerindeki etkileri insan kolon kanseri Caco-2 ve HCT-116 hücrelerine karşı araştırıldı. Elde edilen sonuçlar, büyük nanopartiküllerle karşılaştırıldığında küçük nanopartiküllerin her iki kanser hücresinde de hızlı nanopartikül birikimini desteklediğini gösterdi. Küçük nanopartiküller, büyük nanopartiküllere göre 48 saat içinde daha düşük yarı-maksimum inhibisyon konsantrasyonu (IC50) değerleri ile kanser hücrelerinde daha yüksek sitotoksisite sergiledi. Öte yandan, her iki nanopartikül de 72 saate varan inkübasyon süreleri sonrası benzer IC50 değerleri gösterdi. Ayrıca, küçük nanopartiküller 24 saatte apoptotik hücrelerin sayısını artırırken, büyük nanopartiküllerin 72 saatlik süre içerisinde apoptozu indüklediği belirlendi. Bu gözlemler, küçük boyutlu ilaç taşıma sistemlerinin, büyük boyutlu ilaç taşıma sistemleri ile karşılaştırıldığında, insan kolon kanseri hücrelerinde kemoterapötik ilaçların antikanser etkilerini artırmada daha verimli olduğunu göstermektedir.

Using Inorganic Nanoparticle-Based Drug Delivery Systems against Human Colon Cancer Cells: Effect of Particle Size on Anticancer Activity

Today’s nanoparticle technology enables the synthesis of nanoparticle-based drug delivery systems with desired size, shape, and materials especially for the applications of cancer nanomedicine. Thereby, understanding impact of particle sizes on anticancer activity will contribute to development of new drug delivery systems in cancer therapy. For this reason, in this study, two different sized nanoparticles (with ~55 and 314 nm) were used as drug delivery systems and the effects of their size on the cellular uptake, cytotoxicity and apoptosis were investigated against the human colon carcinoma Caco-2 and HCT-116 cells. The results demonstrated that small nanoparticles promoted fast nanoparticle accumulation in both cancer cells in comparison to large particles. Small nanoparticles exhibited higher cytotoxicity in cancer cells with lower half maximal inhibitory concentration (IC50) values than large nanoparticles in 48 h. On the other hand, both nanoparticles showed similar IC50 values after 72 h prolonged exposure. Moreover, it was found that small nanoparticles increased the number of apoptotic cells in 24 h, whereas large nanoparticles induced apoptosis when exposure time increased to 72 h. These observations show that small sized drug delivery systems could be more efficient for improving the anticancer effects of chemotherapeutic drugs against human colon carcinoma as compared to large sized drug delivery systems.

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Politeknik Dergisi-Cover
  • ISSN: 1302-0900
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 1998
  • Yayıncı: GAZİ ÜNİVERSİTESİ
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