Emine ŞEN, Semanur ÖZDEMİR, Erdal UĞUZDOĞAN

Meyve kabuğu atıklarından pektin ekstraksiyonu ve karakterizasyonu

Çalışmada; mandalina, nar ve bal kabağı kabukları gibi meyve kabuğu atıkları kullanılarak geleneksel asit ekstraksiyonu yöntemi ile pektin ekstrakte edilmiştir. Farklı meyve kabukları kullanılarak ve ekstraksiyon koşulları değiştirilerek elde edilen pektinler karakterize edilerek ticari pektin ile karşılaştırılmıştır. Ham madde olarak üç farklı meyve kabuğu, ekstraksiyon ortamı için iki farklı asit türü (sitrik asit ve hidroklorik asit) ve iki farklı ekstraksiyon sıcaklığı (80 °C ve 90 °C) gibi değişen parametrelerle pektin ekstraksiyonları gerçekleştirilmiştir. Değiştirilen bu parametrelerin elde edilen pektin verimine ve pektinin özelliklerine etkisi incelenmiştir. Ekstrakte edilen pektin örneklerinin; nem ve kül içeriği, FTIR analizleri, esterleşme derecesi, eşdeğer ağırlığı, metoksil içeriği ve anhidroüronik asit içeriği belirlenmiştir. Çalışmada elde edilen pektin verimlerinin; mandalina kabuğu için %4.70-14.50, nar kabuğu için %4.40-9.25 ve bal kabağı kabuğu için ise %2.10-4.80 değerleri arasında değiştiği bulunmuştur. Pektin ekstraksiyonu için çalışmada kullanılan meyve kabukları arasında en yüksek pektin verimi bir turunçgil meyvesi olan mandalina kabuğundan %14.50 olarak elde edilmiştir. Yapılan analiz ve karakterizasyon sonuçları; çalışmada elde edilen pektinlerin çoğunlukla yüksek metoksilli pektinler olduğunu göstermektedir. Farklı meyve kabuklarından ekstrakte edilen pektinlerin verimleri, ekstrakte edilen pektinlerin özellikleri ve çevresel etkiler göz önüne alındığında ekstraksiyon ortamı olarak sitrik asitin kullanımının ve ekstraksiyon sıcaklığı olarak 90 °C sıcaklığın seçilmesinin daha uygun olduğu sonucuna varılmıştır.

Anahtar Kelimeler:

Pektin, Asit ekstraksiyonu, Mandalina kabuğu, Nar kabuğu, Bal kabağı kabuğu, Meyve kabuğu

Extraction and characterization of pectin from waste of fruit peels

In this study; pectin was extracted using traditional acid extraction method using fruit peel waste such as mandarin, pomegranate and pumpkin peels. The pectins obtained by using different fruit peels and changing the extraction conditions were characterized and compared with commercial pectin. Pectin extractions were carried out with varying parameters such as three different fruit peels as raw materials, two different acid types (citric acid and hydrochloric acid) for the extraction medium and two different extraction temperatures (80 °C and 90 °C). The effects of these parameters on the pectin yield and properties of pectin were investigated. Moisture and ash content, FTIR analysis, esterification degree, equivalent weight, methoxyl content and anhydrouronic acid content were determined for the characterisation of the extracted pectin samples. The pectin yields were found to be; 4.70-14.50% for mandarin peel, 4.40-9.25% for pomegranate peel and 2.10-4.80% for pumpkin peel. Among the fruit peels used in the study for pectin extraction, the highest pectin yield was obtained as 14.50% from the mandarin peel, which is a citrus fruit. The results of the analysis and characterization show that the samples are mainly composed of high methoxy pectins. Considering the yields of extracted pectins from different fruit peels, their properties and environmental effects, it was concluded that it is more appropriate to use citric acid as the extraction medium and to select the extraction temperature of 90 °C.

Keywords:

Pectin, Acid extraction, Mandarin peel, Pomegranate peel, pumpkin Peel, Fruit peel,

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[1] Naqash F, Masoodi F, Rather S, Wani S, Gani A. “Emerging concepts in the nutraceutical and functional properties of pectin: A review”. Carbohydrate Polymers, 168, 227-239, 2017.
[2] Grassino AN, Barba FJ, Brncic M, Lorenzo JM, Lucini L, Brncic SR. “Analytical tools used for the identification and quantification of pectin extracted from plant food matrices, wastes and by-products: A review”. Food Chemistry, 266, 47-55, 2018.
[3] Canteri-Schemin MH, Fertonani HCR, Waszczynskyj N, Wosiacki G. “Extraction of pectin from apple pomace”. Brazilian Archives of Biology and Technology, 48(2), 259-266, 2005.
[4] Willats WG, McCartney L, Mackie W, Knox JP. “Pectin: cell biology and prospects for functional analysis”. Plant Molecular Biology, 47(1-2), 9-27, 2001.
[5] Yapo BM. “Pectic substances: from simple pectic polysaccharides to complex pectins-A new hypothetical model: A review”. Carbohydrate Polymers, 86, 373-385, 2011.
[6] Sriamornsak P. “Chemistry of pectin and its pharmaceutical uses: A review”. Silpakorn University International Journal, 3(1-2), 206-228, 2003.
[7] Oakenfull DG, Scott A. “Hydrophobic interaction in the gelation of high methoxyl pectins”. Journal of Food Science, 49(4), 1093-1098, 1984.
[8] May CD. “Industrial pectins: Sources, production and applications”. Carbohydrate Polymers, 12, 79-99, 1990.
[9] El-Nawawi SA, Heikel YA. “Factors affecting gelation of high-ester citrus pectin”, Process Biochemistry, 32(5), 381-385, 1997.
[10] Evranuz Ö. Ayçiçeği Tablalarından Pektin Eldesinde Pektin Kalitesini Etkileyen Faktörler ve Konu ile İlgili Teknolojik Öneriler. Doktora Tezi, İstanbul Teknik Üniversitesi, İstanbul, Türkiye, 1985.
[11] Voragen AGJ, Coenen GJ, Verhoef RP, Schols HA. “Pectin, the versatile polysaccharide polysaccharide present in plant cell walls”. Structural Chemistry, 20, 263-275, 2009.
[12] Kanse NG, Chirag S, Swapnil S, Vishal S. “Extraction of pectin from orange peels and its applications: A review”. International Journal of Innovative Research in Science, 6(9), 19452-19457, 2017.
[13] Pinheiro ER, Silva IMDA, Gonzaga LV, Lover ER, Teófilo RF, Ferreira MMC, Amboni RDMC. “Optimization of extraction of high-ester pectin from passion fruit peel (Passiflora edulis flavicarpa) with citric acid by using response surface methodology”. Bioresource Technology, 99, 5561-5566, 2008.
[14] Silva DC, Freitas ALP, Pessoa CDS, Paula RCM, Mosque JX, Leal LKAM, Brito GAC, Gonçalves DO, Viana GS. “Pectin from Passiflora edulis Shows anti-inflammatory action as well as hypoglycemic and hypotriglyceridemic properties in diabetic rats”. Journal of Medicinal Food, 14, 1118-1126, 2011.
[15] Jackson CL, Dreaden TM, Theobald LK, Tran NM, Beal TL, Eid M, Gao MY, Shirley RB, Stoffel MT, Kumar MV, Mohnen D. “Pectin induces apoptosis in human prostate cancer cells: Correlation of apoptotic function with pectin structure”. Glycobiology, 17, 805-819, 2007.
[16] Wicker L, Kim Y, Kim M, Thirkield B, Lin Z, Jung J. “Pectin as a bioactive polysaccharide-Extracting tailored function from less”. Food Hydrocolloids, 42, 251-259, 2014.
[17] Annadurai G, Juang RS, Lee DJ. “Adsorption of heavy metals from water using banana and orange peels”. Water Science and Technology, 47, 185-190, 2003.
[18] Kumar R, Kumar A, Chauhan KR, Gupta R, Ahn JH, Chauhan GS. “Removal of as (V) from water by pectin based active hydrogels following geochemical approach”. Bioresource Technology, 100, 1474-1477, 2009.
[19] Singha NR, Karmakar M, Mahapatra M, Mondal H, Dutta A, Roy C, Chattopadhyay PK. “Systematic synthesis of pecting-(sodium-acrylate-co-N-isopropylacrylamide) interpenetrating polymer network for superadsorption of dyes/M (ii): Determination of physicochemical changes in loaded hydrogels”. Polymer Chemistry, 8, 3211-3237, 2017.
[20] Ji F, Li J, Qin Z, Yang B, Zhang E, Dong D, Wang J, Wen Y, Tian L, Yao F. “Engineering pectin-based hollow nanocapsules for delivery of anticancer drug”. Carbohydrate Polymers, 177, 86-96, 2017.
[21] Krivorotova T, Cirkovas A, Maciulyte S, Staneviciene R, Budriene S, Serviene E, Sereikaite J. “Nisin-loaded pectin nanoparticles for food preservation”. Food Hydrocolloids, 54, 49-56, 2016.
[22] Mohnen D. “Pectin structure and biosynthesis”. Current Opinion in Plant Biology, 11, 266-277, 2008.
[23] Cuq B, Contard N, Guilbert S. Edible films and coatings as active layers. Editor: Rooney ML, an imprint of Chapman and Hall, Active Food Packaging Blackie Academic & Professional, 111-142, Glasgow, UK, 1995.
[24] Freitas CMP, Sousa RCS, Dias MMS, Coimbra JSR. “Extraction of pectin from passion fruit peel”. Food Engineering Reviews, 12, 460-472, 2020.
[25] Sayah M, Chabir R, Benyahia H, Rodi Kandri Y, Ouazzani Chahdi F, Touzani H, Errachidi F. “Yield, esterification degree and molecular weight evaluation of pectins isolated from orange and grapefruit peels under different conditions”. Plos One, 11 (9), e0161751, 2016.
[26] Abid M,Renard CMGC, Watrelot AA, Fendri I, Attia H, Ayadi MA. “Yield and composition of pectin extracted from Tunisian pomegranate peel”. International Journal of Biological Macromolecules, 93, 186-194, 2016.
[27] Kaya M, Sousa AG, Crépeau MJ, Sørensen SO, Ralet MC. “Characterization of citrus pectin samples extracted under different conditions: influence of acid type and pH of extraction”. Annals of Botany, 114, 1319-1326, 2014.
[28] Gazala K, Masoodi FA, Masarat HD. “Extraction and characterisation of pectin from two apple juice concentrate processing plants”. International Food Research Journal, 24(2), 594-599, 2017.
[29] Kurita O, Fujiwara T, Yamazaki E. “Characterization of the pectin extracted from citrus peel in the presence of citric acid”. Carbohydrate Polymers, 74(3), 725-730, 2008.
[30] Garna H, Mabon N, Robert C, Cornet C, Nott K, Legros H, Wathelet B, Paquot M. “Effect of extraction conditions on the yield and purity of apple pomace pectin precipitated but not washed by alcohol”. Journal of Food Science, 72(1), C1-C9, 2007.
[31] Rutkowska M, Namieśnik J, Konieczka P. UltrasoundAssisted Extraction. Editors: Pena-Pereira F, Tobiszewski M. The Application of Green Solvents in Separation Processes, 301-324, Elsevier, Amsterdam, UK, 2017.
[32] Moorthy I, Maran J, Ilakya S, Anitha S, Sabarima S, Priya B. “Ultrasound assisted extraction of pectin from waste artocarpus heterophyllus fruit peel”. Ultrasonics Sonochemistry, 34, 525-530, 2017.
[33] Košťálová Z, Aguedo M, Hromádková Z. “Microwaveassisted extraction of pectin from unutilized pumpkin biomass”. Chemical Engineering and Processing: Process Intensification, 102, 9-15, 2016.
[34] Hosseini S, Khodaiyan F, Yarmand M. “Optimization of microwave assisted extraction of pectin from sour orange peel and its physicochemical properties”. Carbohydrate Polymers, 140, 59-65, 2016.
[35] Liew SQ, Teoh WH, Tan CK, Yusoff R, Ngoh GC. “Subcritical water extraction of low methoxyl pectin from pomelo (Citrus grandis (L.) Osbeck) peels”. International Journal of Biological Macromolecules, 116, 128-135, 2018.
[36] Muñoz-Almagro N, Valadez-Carmona L, Mendiola JA, Ibáñez E, Villamiel M. “Structural characterisation of pectin obtained from cacao pod husk. Comparison of conventional and subcritical water extraction”. Carbohydrate Polymers, 217, 69-78, 2019.
[37] Dominiak M, Søndergaard K, Wichmann J, Vidal-Melgosa S, Willats W, Meyer A, Mikkelsen J. “Application of enzymes for efficient extraction, modification, and development of functional properties of lime pectin”. Food Hydrocolloids, 40, 273-282, 2014.
[38] Sabater C, Corzo N, Olano A, Montilla A. “Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L”. Carbohydrate Polymers, 190, 43-49, 2018.
[39] Levigne S, Ralet MC, Thibault JF. “Characterisation of pectins extractedfrom fresh sugar beet under different conditions using an experimental design”. Carbohydrate Polymers, 49(2), 145-153, 2002.
[40] Kulkarni SG, Vijayanand P. “Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f flavicarpa L.)”. Food Science and Technology, 43(7), 1026-1031, 2010.
[41] Chan SY, Choo WS, Young DJ, Loh XJ. “Pectin as a rheology modifier: Origin, structure, commercial production and rheology”. Carbohydrate Polymers, 161, 118-139, 2017.
[42] Qiu L, Zhao G, Wu H, Jiang L, Li X, Liu J. “Investigation of combined effects of independent variables on extraction of pectin from banana peel using response surface methodology”. Carbohydrate Polymers, 80, 326-331, 2010.
[43] Miyamoto A, Chang KC. “Extraction and characterization of pectin from sunflower head residue”. Journal of Food Science, 5, 1439-1443, 1992.
[44] Abid M, Cheikhrouhou S, Renard CMGC, Bureau S, Cuvelier G, Attia H, Ayadi MA. “Characterization of pectins extracted from pomegranate peel and their gelling properties”. Food Chemistry, 215, 318-325, 2017.
[45] Pereira PHF, Oliveira TÍS, Rosa MF, Cavalcante FL, Moates GK, Wellner N, Waldron KW, Azeredo KMC. “Pectin extraction from pomegranate peels with citric acid”. International Journal of Biological Macromolecules, 88, 373-379, 2016.
[46] Jafari F, Khodaiyan F, Kiani H, Hosseini SS. “Pectin from carrot pomace: Optimization of extraction and physicochemical properties”. Carbohydrate Polymers, 157, 1315-1322, 2017.
[47] Grassino AN, Brnčić M, Vikić-Topić D, Roca S, Dent M, Brnčić SR. “Ultrasound assisted extraction and characterization of pectin from tomato waste”. Food Chemistry, 198, 93-100, 2016.
[48] Morales-Contreras BE, Contreras-Esquivel JC, Wicker L, Ochoa-Martínez LA, Morales-Castro J. ”Husk tomato (Physalis ixocarpa Brot.) waste as a promising source of pectin: Extraction and physicochemical characterization”. Journal of Food Science, 82, 1594-1601, 2017.
[49] Urias-Orona V, Rascón-Chu A, Lizardi-Mendoza J, CarvajalMillán E, Gardea AA, Ramírez-Wong B. “A novel pectin material: Extraction, characterization and gelling properties”. International Journal of Molecular Sciences, 11, 3686-3695, 2010.
[50] Chaharbaghi E, Khodaiyan F, Hosseini SS. “Optimization of pectin extraction from pistachio green hull as a new source”. Carbohydrate Polymers, 173, 107-113, 2017.
[51] Faravash RS, Ashtiani FZ. “The influence of acid volume, ethanol-to extract ratio and acid-washing time on the yield of pectic substances extraction from peach pomace”. Food Hydrocolloids, 22, 196-202, 2008.
[52] Ranganna, S. Pectin, Handbook Of Analysis and Quality Control For Fruit and Vegetable Products, Tata McGraw Hill, New Delhi, India, 2008.
[53] Bochek AM, Zabivalova NM, Petropavlovskii GA. “Determination of the esterification degree of polygalacturonic acid”. Russian Journal of Applied Chemistry, 75, 796-799, 2011.
[54] Yapo BM, Robert C, Etienne I, Wathelet B, Paquot M. “Effect of extraction conditions on the yield, purity and surface properties of sugar beet pulp pectin extracts”. Food Chemistry, 100, 1356-1364, 2007.
[55] Gutöhrlein F, Drusch S, Schalow S. “Extraction of low methoxylated pectin from pea hulls via RSM”. Food Hydrocolloids, 102, 105609, 2020.
[56] Torkova AA, Lisitskaya KV, Filimonov IS, Glazunova OA, Kachalova GS, Golubev VT. “Physicochemical and functional properties of Cucurbita maxima pumpkin pectin and commercial citrus and apple pectins: A comparative evaluation”. Plos One, 13(9), 1-25, 2018.
[57] Ramachandran P, Dhiman AK, Attri S. “Extraction of pectin from ripe pumpkin (Cucurbita moschata Duch ex. Poir) using eco-friendly technique”. Indian Journal of Ecology, 4(6), 685-689, 2017.
[58] Zhao J, Zhang F, Liu X, Ange K, Zhang A, Li Q. “Isolation of a lectin binding rhamnogalacturonan-I containing pectic polysaccharide from pumpkin”. Carbohydrate Polymers, 163, 330-336, 2017.
[59] Yoo SH, Lee BH, Lee H, Lee S, Bae IY, Lee HG. “Structural characteristics of pumpkin pectin extracted by microwave heating”. Journal of Food Science, 77(11), 1169-1173, 2012.
[60] Baississe S, Fahloul D. “Rheological behavior and electrokinetic properties of pectin extracted from pumpkin (Cucurbita maxima) pulp and peel using hydrochloric acid solution”. Chemical Papers, 72(10), 2647-2658, 2018.
[61] Jun HI, Lee CH, Song GS, Kim YS. “Characterization of the pectic polysaccharides from pumpkin peel”. Journal of Food Science and Technology, 39(5), 554-561, 2006.
[62] Güzel M, Akpınar Ö. “Turunçgil kabuklarından elde edilen pektinlerin karakterizasyonu ve karşılaştırılması”. Akademik Gıda, 15, 17-28, 2017.
[63] Han HS, Song KB. “Antioxidant activities of mandarin (Citrus unshiu) peel pectin films containing sage (Salvia officinalis) leaf extract”. International Journal of Food Science and Technology, 55, 3173-3181, 2020.
[64] Cho CW, Lee DY, Kim CW. “Concentration and purification of soluble pectin from mandarin peels using cross flow micro-filtration system”. Carbohydrate Polymers, 54(1), 21-26, 2003.
[65] Daud NZA, Said BNM, Ja’afar F, Yasin HM, Kusrini E, Usman A. “pH-dependent yield and physicochemical properties of pectin isolated from citrus maxima”. International Journal of Technology, 10(6), 1131-1139, 2019.
[66] Chen Y, Zhang JG, Sun HJ, Wei ZJ. “Pectin from Abelmoschus esculentus: Optimization of extraction and rheological properties”. International Journal of Biological Macromolecules, 70, 498-505, 2014.
[67] Wang W, Ma X, Xu Y, Cao Y, Jiang Z, Ding T, Ye X, Liu D. “Ultrasound-assisted heating extraction of pectin from grapefruit peel: Optimization and comparison with the conventional method”. Food Chemistry, 178, 106-114, 2015.
[68] Begum R, Aziz MG, Uddin MB, Yusof YA. “Characterization of jackfruit (Artocarpus heterophyllus) waste pectin as influenced by various extraction conditions”. Agriculture and Agricultural Science Procedia, 2, 244-251, 2014.
[69] Kozarski M, Klaus A, Niksic M, Jakovljevic A, Helsper JPFG, Griensven LJLD. “Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus”. Food Chemistry, 129, 1667-1675, 2011.
[70] Cerna M, Barros AS, Nunes A, Rocha SM, Delgado I, Copikova J. “Use of FT-IR spectroscopy as a tool for the analysis of polysaccharide food additives”. Carbohydrate Polymers, 37, 241-248, 2003.
[71] Rodsamran P, Sothornvit R. “Microwave heating extraction of pectin from lime peel: Characterization and properties compared with the conventional heating method”. Food Chemistry, 278, 364-372, 2019.
[72] Kute AB, Mohapatra D, Kotwaliwale N, Giri SK, Sawant BP. “Characterization of pectin extracted from orange peel powder using microwave-assisted and acid extraction methods”. Agricultural Research, 9(2), 241-248, 2020.
[73] Virk BS, Sogi DS. “Extraction and characterization of pectin from apple pomace (Malus Pumila Cv Amri) peel waste”. International Journal of Food Properties, 7, 1-11, 2004.
[74] Food Chemical Codex. IV monographs, Washington DC: National Academy Press, 283, 1996.
[75] Ismail NSM, Ramli N, Hani NM, Meon Z. “Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions”. Sains Malaysiana, 41, 41-45, 2012.
[76] Kumar A, Chauhan GS. “Extraction and characterization of pectin from apple pomace and its evaluation as lipase (steapsin) inhibitor”. Carbohydrate Polymers, 82(2), 454-459, 2010.
[77] Devi WE. “Extraction of pectin from citrus fruit peel and its utilization in preparation of jelly”. International Journal of Engineering Research & Technology, 3(5), 1925-1932, 2014.
[78] Tang PY, Kek TS, Gan CZ, Hee CY, Chong CH, Woo KK. “Yield and some chemical properties of pectin extracted from the peels of dragon fruit [Hylocereus polyrhizus (Weber) Britton and Rose]”. The Philippine Agricultural Scientist, 94(3), 307-311, 2011.
[79] Yapo BM. “Biochemical characteristics and gelling capacity of pectin from yellow passion fruit rind as affected by acid extractant nature”. Journal of Agricultural and Food Chemistry, 57(4), 1572-1578, 2009.