Bilgin KAYGISIZ, Şule AYDIN, CAFER YILDIRIM, Hadi KARİMKHANİ, Setenay ÖNER, Fatma Sultan KILIÇ

The Effects of Pregabalin on Gastric Ulcer Formation and Antioxidant Parameters

Pregabalin, a drug used in epilepsy, anxiety, neuropathic pain is reported to have analgesic effects in inflammatory pain. We aimed to investigate whether pregabalin have gastric side effects and to compare with a non steroidal antiinflammatory drug (NSAID) in rats. The effects of pregabalin on antioxidant levels, which are suggested to protect against gastric mucosal damage were also studied. Pregabalin 30, 50, 100 mg/kg, indomethacin 5 mg/kg (reference-NSAID), saline (control group) were administered orally for 10 days. At the end of 10 day treatment, rats were sacrificed, gastric tissues were removed out, mucus secretion was determined spectrophotometrically, ulcer index was scored from score 0:(no-petechia) to score 3:(petechia>5mm). Also, to evaluate the antioxidant effects of pregabalin, malondialdehyde (MDA) levels, catalase and superoxide dismutase (SOD) activities in gastric tissue were studied. Pregabalin 50 mg/kg and 100 mg/kg similar to indomethacin significantly reduced mucus secretion and increased ulcer index compared to control while pregabalin 30 mg/kg did not. Pregabalin 30 mg/kg and 100 mg/kg decreased SOD and catalase levels. Pregabalin 100 mg/kg dose increased MDA levels. 50 mg/kg and 100 mg/kg pregabalin showed gastric side effects as reduced mucus secretion and ulcer formation similar to indomethacin and 30 mg/kg pregabalin may be reasonable dose without showing gastric side effects. Pregabalin 50 mg/kg seems to have enhancing effects on antioxidant levels.

Pregabalinin Gastrik U lser Oluşumu ve Antioksidan Parametreler U zerine Etkileri

Pregabalin epilepsi, ankisiyete ve nöropatik ağrıda kullanılan analjezik etkiye sahip antiinflamatuvar bir ajandır. Biz çalışmamızda pregabalinin sıçanlarda mide üzerine yan etkilerinin olup olmadığını ve bir non-steridal antiinflamatuvar bir ajan ile etkisini karşılaştırmayı amaçladık. Pregabalinin gastrik mukozal hasara karşı koruyucu olduğu düşünülen antioksidan seviyeleri üzerine olan etkilerini de araştırdık. Pregabalin 30, 50, 100 mg/kg, indometasin 5 mg/kg (referans-NSAID), salin (kontrol grubu) 10 gün boyunca oral olarak uygulandı. 10 günlük tedavinin sonunda, sıçanlar sakrifiye edildi, mide dokuları çıkarıldı, mukus salgılanması spektrofotometrik olarak belirlendi, ülser indeksi 0'dan (no-peteşi) skor 3'e (peteşi> 5mm) kadar skorlandı. Ayrıca pregabalinin mide dokusundaki antioksidan etkilerini değerlendirmek üzere malondialdehid (MDA), katalaz ve süperoksit dismutaz (SOD) aktiviteleri çalışıldı. Pregabalin 50 mg/kg ve 100 mg/kg dozları kontrol grubu ile karşılaştırıldığında indometazine benzer şekilde anlamlı olarak mukus salgılanmasını azalttı ve ülser indeksini artırdı. Pregabalin 30 mg/kg dozunda bu etki görülmedi. Pregabalin 30 mg/kg ve 100 mg/kg SOD ve katalaz seviyelerini düşürüyorken pregabalin 100 mg/kg dozunda MDA seviyeleri artmıştır. 50 mg/kg and 100 mg/kg pregabalin mukus salgılanmasını ve ülser oluşumu indometazin 30 mg/kg dozuna benzer şekilde azaltarak gastrik yan etkiler göstermiştir. Pregabalin 30 mg/kg dozu ise gastrik yan etkilerin ortaya çıkmadığı uygun doz olabilir. Pregabalin 50 mg/kg dozunun ise antioksidan seviyelerini artırıcı etkiye sahip olduğu görülmüştür.

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