Sabiha ŞAHİN, Ufuk UYLAS, VAROL ŞAHİNTÜRK, Ozkan ALATAS

Protective Effect of Carnosine on Hepatic Ischemia-Reperfusion Injury in Rats

This study investigated the antioxidant effects of carnosine (CAR) in rats exposed to hepatic ischemia-reperfusion (I/R) injury using biochemical and histopathological evaluation. Twenty-four Sprague-Dawley male rats weighing 200–250 g were used to investigate the antioxidant effects of carnosine on the liver. Rats were randomly divided into the following groups: sham (control) group (G1), hepatic I/R group (G2), and hepatic I/R treated with 100 mg/kg CAR group (G3). Rats in the control group underwent only laparotomy and catheterisation. Rats in the other groups received 2 h of reperfusion following 1 h of hepatic ischemia by hepatic artery clamping after laparotomy. Rats in the treatment group received an intraperitoneal (i.p.) injection of 100 mg/kg CAR 60 min before hepatic artery clamping. All rats were monitored for 48 h. Then, they were sacrificed and blood samples were obtained for determination of aspartate transaminase (AST), alanine transaminase (ALT), and liver tissue samples were taken for malondialdehyde (MDA), glutathione (GSH) and catalase (CAT) levels, and for the histopathologic examination as well. Serum AST, ALT and tissue MDA levels were significantly decreased and GSH and CAT levels were significantly higher in the CARtreated group compared to the non-treated group exposed to only I/R injury. Histopathological damage was significantly less in rats which received carnosine than that of non-treated group. We concluded that 100 mg/kg CAR may be effective in preventing the oxidative damage in liver tissue due to I/R injury.

Karaciğ er I skemisinde Karnozinin Koruyucu Etkisi

Bu çalışmada, biyokimyasal ve histopatolojik değerlendirme kullanılarak karaciğer iskemisi-reperfüzyon (İ / R) hasarına maruz kalan sıçanlarda karnosinin (CAR) antioksidan etkileri araştırıldı. Karnosinin karaciğer üzerindeki antioksidan etkilerini araştırmak için 200-250 gr ağırlığındaki yirmi dört Sprague-Dawley erkek sıçan kullanıldı. Sıçanlar rastgele aşağıdaki gruplara ayrıldı: Kontrol grubu (G1), hepatik I / R grubu (G2) ve 100 mg / kg CAR ile tedavi edilen hepatik I / R grubu (G3). Kontrol grubundaki sıçanlara sadece laparotomi ve kateterizasyon uygulandı. Diğer gruplardaki ler, laparotomi sonrası hepatik arter klemplenmesi ile 1 saat hepatik iskemi sonrasında 2 saat reperfüzyona maruz bırakıldı. Tedavi grubundaki farelere, hepatik arter klemplenmeden 60 dakika önce 100 mg / kg CAR 60 dakika intraperitoneal enjeksiyon olarak yapıldı. Tüm sıçanlar 48 saat izlendi. Daha sonra sacrifiye edilip aspartat transaminaz (AST), alanin transaminaz (ALT) tayini için kan örnekleri alındı ve malondialdehit (MDA), glutatyon (GSH) ve katalaz (CAT) seviyelerin ölçmek için ve histopatolojik incelemeiçin karaciğer doku örnekleri alındı. Tedavi grubunda Serum AST, ALT ve doku MDA düzeyleri anlamlı olarak azaldı ve GSH ve CAT düzeyleri CAR ile tedavi edilen grupta, sadece I / R yaralanmasına maruz kalan tedavi edilmeyen grupla karşılaştırıldığında anlamlı olarak daha yüksekti. Karnosin alan sıçanlarda histopatolojik hasar, tedavi edilmeyen gruba göre anlamlı derecede azdı. Bu çalışma sonucunda 100 mg / kg CAR'ın, İ / R hasarı nedeniyle karaciğer dokusunda oksidatif hasarı önlemede etkili olabileceği sonucuna vardık.

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