Evin KOCATÜRK, Eren GUNDUZ, Hüseyin KAYADİBİ, Hava ÜSKÜDAR TEKE, İ. Özkan ALATAŞ

Otolog Nakil İçin Periferik Kan Hematopoietik Kök Hücre Toplamanın Optimal Zamanı, Olgunlaşmamış Granülositler ve Çekirdekli Kırmızı Kan Hücreleri Tarafından Tahmin Edilebilir

Başarılı bir nakil için kök hücre toplamaya başlama zamanı çok önemlidir ve bu zaman akış sitometrisi ile analiz edilen periferik kan CD34+ hücre sayısına göre belirlenir. Bu çalışmada, kök hücre toplamanın optimal zamanını belirlemek için yeni tam kan sayımı parametrelerinin rolünü bulmayı amaçladık. Çalışmaya kök hücre mobilizasyonu uygulanan 86 hasta dahil edildi. Aynı gün periferik kan CD34+ hücreleri ve tam kan sayımı yapıldı. Periferik kan CD 34+ hücre sayısı ≤ 20/μL ve > 20/μL olan hastalar sırasıyla Grup 1 ve Grup 2 olarak belirlendi. İki grup arasındaki tam kan sayımı parametreleri farkı ve bu parametrelerin periferik kan CD34+ hücre sayısı ile ilişkisi değerlendirildi. İmmatür granülosit oranı, çekirdekli eritrosit oranı ve sayısının periferik kan CD34+ hücre sayısı ile pozitif korelasyon gösterdiği ve bu parametrelerin iki grup arasında anlamlı olarak farklı olduğu bulundu (sırasıyla P< 0.001, P= 0.011 ve P= 0.012). Olgunlaşmamış granülositler ve çekirdekli kırmızı kan hücreleri oranları kullanılarak formüle edilen yeni indeks, iki grup arasında önemli ölçüde farklıydı (P<0.001) ve kök hücre toplama zamanını belirlemek için en yüksek tanı doğruluğuna sahipti (AUC= 0.766). İmmatür granülositler ve çekirdekli kırmızı kan hücreleri, akış sitometrisine göre periferik kan kök hücre toplama zamanını daha hızlı ve daha ucuza belirlemek için kullanılabilir.

Anahtar Kelimeler:

Autologous transplantation, Stem cell mobilization, CD34+ cells, Immature granulocytes, Nucleated red blood cells

The Optimal Time of Peripheral Blood Hematopoietic Stem Cell Collection for Autologous Transplantation May Be Predicted By Immature Granulocytes and Nucleated Red Blood Cells

The time of initiating stem cell collection is very important for successful transplantation and is determined according to peripheral blood CD34+ cell count analysed by flow cytometry. In this study, we aimed to find the role of new complete blood count parameters to determine the optimal time of stem cell collection. Eighty-six patients who underwent stem cell mobilization were included in the study. Peripheral blood CD34+ cells and complete blood count were analysed on the same day. Patients with peripheral blood CD 34+ cell counts ≤ 20/μL and > 20/μL were determined as Group 1 and Group 2, respectively. The difference of CBC parameters between 2 groups and the relationship of these parameters with peripheral blood CD34+ cell counts were evaluated. It was found that immature granulocytes ratio, nucleated red blood cells ratio and count were positively correlated with peripheral blood CD34+ cell count and these parameters were significantly different between two groups (P< 0.001, P= 0.011 and P= 0.012, respectively). The new index formulated by using immature granulocytes and nucleated red blood cells ratios was significantly different between two groups (P<0.001), and had the highest diagnostic accuracy to determine the time of stem cell collection (AUC= 0.766). Immature granulocytes and nucleated red blood cells can be used to determine the time of peripheral blood stem cell collection faster and cheaper compared to flow cytometry.

Keywords:

Autologous transplantation, Stem cell mobilization, CD34+ cells, Immature granulocytes, Nucleated red blood cells,

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