Salim NEŞELİOĞLU, Yasemin EREN, Ebru Bilge DİRİK, Emine Feyza YURT, Serpil ERDOGAN, Orhan DENİZ, Özcan EREL

Huzursuz bacak sendromlu hastalarda hastalık şiddeti ve tiyol-disülfid homeostazı arasındaki güçlü ilişki

Amaç: Bu çalışma, primer huzursuz bacak sendromu (RLS) hastalarında yeni bir oksidatif stres belirteci olan tiyol-disülfid homeostazisini araştırmak amacıyla yapıldı.Gereç ve Yöntem: Otuz sekiz hasta ve 43 sağlıklı birey çalışmaya alındı. Serum nativ tiyol, total tiyol, disülfit ve disülfid / nativ tiyol oranları yeni bir otomatik yöntem kullanılarak ölçülmüştür. RLS tanısı Uluslararası Huzursuz Bacak Sendromu Çalışma Grubu (IRLSSG) tarafından önerilen kriterlere gore konuldu ve RLS şiddeti IRLSSG Şiddet Ölçeği kullanılarak değerlendirildi.Bulgular: Serum nativ tiyol ve total tiyol düzeyleri anlamlı derecede düşük (sırasıyla p<0.001; p=0.007), disülfid ve disülfid / nativ tiyol oranı anlamlı olarak daha yüksekti (sırasıyla p=0.017; p<0.001). RLS kontrol grubu ile karşılaştırıldı. IRLSSG Önem Ölçeği ve tiyol-disülfid homeostasisi parametreleri arasında anlamlı korelasyonlar bulundu (r=-0.56, nativ tiyol için p<0.001; total tiyol için r=-0.51, p<0.001; disülfid için r=0.37, p=0.020); r=0.60, disülfid / nativ tiyol yüzdesi oranı için p<0.001). Sonuçlar: Azalmış nativ tiyol, artan disülfid seviyeleri ve artmış disülfid / nativ tiyol oranı, tiyol-disülfür dengesinin oksidasyon tarafına kaydığını göstermektedir. IRLSSG Önem Ölçeği ve tiyol-disülfid homeostasisi parametreleri arasındaki güçlü korelasyonlar, homeostazın primer RLS'nin patogenezinde rol oynayabileceğini göstermektedir.

Anahtar Kelimeler:

Disulphide, oxidative stress, restless legs syndrome, thiol, thiol-disulphide homeostasis

The strong relationship between disease severity and thiol-disulphide homeostasis in patients with restless legs syndrome

Objective: This study was conducted to investigate thiol-disulphide homeostasis, a novel oxidative stress marker in primary restless legs syndrome (RLS) patients.Material and Method: Thirty-eight patients and 43 healthy subjects were included in the study. Serum native thiol, total thiol, disulphide and disulphide / native thiol percent ratio levels were measured by using a novel automated method. Diagnosis of RLS was based on the criteria proposed by the International Restless Legs Syndrome Study Group (IRLSSG), and RLS severity was assessed using the IRLSSG Severity Scale.Results: Serum native thiol and total thiol levels were significantly lower (p< 0.001; p= 0.007, respectively) while disulphide and disulphide/native thiol percent ratio levels significantly higher (p= 0.017; p< 0.001, respectively), in patients with RLS compared with control group. Significant correlations were found between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters (r= -0.56, p< 0.001 for native thiol; r= -0.51, p< 0.001 for total thiol; r= 0.37, p= 0.020 for disulphide and r= 0.60, p< 0.001 for disulphide/native thiol percent ratio). Conclusions: Decreased native thiol, increased disulphide levels and increased disulphide/native thiol percent ratio show that thiol-disulphide balance shifted to the oxidation side. Strong correlations between the IRLSSG Severity Scale and thiol-disulphide homeostasis parameters indicate that homeostasis may have a role in the pathogenesis of the Primary RLS.

Keywords:

Disulphide, oxidative stress, restless leg syndrome, thiol, thiol-disulphide hemeostasis,

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