ZELİHA YILDIRIM, Yaselin İLK, METİN YILDIRIM

ENTEROSİN KP'NİN İNHİBİTÖR AKTİVİTESİ ÜZERİNE SODYUM KLORÜR VE ASİTLİĞİN ETKİSİ

Bu çalışmada, Enterococcus faecalis KP tarafından üretilen enterosin KP'nin inhibitör aktivitesi ve spektrumu üzerine NaCl ile pH'nın etkisi incelenmiştir. Test mikroorganizması olarak Lactobacillus plantarum, Listeria monocytogenes, Escherichia coli O157:H7 ve Salmonella Typhimurium kullanılmıştır. Araştırma sonucunda %4-7 oranında NaCl ile birlikte kullanılan enterosin KP'nin inhibitör aktivitesinin arttığı belirlenmiştir. Ayrıca düşük pH (5,0-5,3) koşullarının da enterosin KP'nin aktivitesinin artmasına ve hatta Escherichia coli O157:H7'nin de enterosin KP'ye karşı duyarlı hale gelmesine yol açtığı gözlenmiştir. Buna karşın, asidik ve nötral pH değerleri enterosin KP aktivitesini etkilememiştir. Sonuç olarak, enterosin KP düşük pH koşullarında ve NaCl ile birlikte kullanıldığında sinerji etkisinin oluştuğu ve buna paralel olarak inhibitör aktivitesinin arttığı belirlenmiştir.

EFFECT OF SODIUM CHLORIDE AND ACIDITY ON INHIBITORY ACTIVITY OF ENTEROCIN KP

In this study, the effect of NaCl and pH on the inhibitory spectrum and activity of enterocin KP produced by Enterococcus faecalis KP was determined. Lactobacillus plantarum, Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella Typhimurium were used as target microorganisms. At the end of the study, the inhibitory activity of enterocin KP was increased in the presence of NaCl at concentrations of 4-7%. Also, low pH (5.0-5.3) condition either caused an increase enterocin KP activity or rendered Escherichia coli O157:H7 sensitive to enterocin KP. However, acidic and neutral pH did not affect its activity. In conclusion, a synergist effect was occurred and inhibitory activity of enterocin KP increased when it was used in combination with NaCl or under acidic condition.

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