Akut Yorucu Egzersiz Yaptırılan Sıçanlarda Melatoninin Elektrokardiyografik Veriler Üzerindeki Etkilerinin Araştırılması

Amaç: Akut yorucu egzersiz kardiyovasküler sistem (KVS) için ilave bir yük oluşturmaktadır. Melatonin ise KVS üzerine koruyucu etkileri olduğu bilinen bir hormondur. Bu çalışma ile akut yorucu egzersiz uygulanan sıçanlarda KVS’de meydana gelen değişikliklerin elektrokardiyografi (EKG) verileri üzerinden değerlendirilmesi ve melatoninin koruyucu etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Çalışma 3-5 aylık 28 adet Wistar Albino sıçan üzerinde yürütüldü ve 4 ayrı grup oluşturuldu. Kontrol grubuna sadece taşıyıcı çözelti uygulandı ve herhangi bir egzersiz yapılmadı. Egzersiz, egzersiz+melatonin (egzersizden önce 10 mg/kg intraperitonal melatonin enjeksiyonu) ve egzersiz+melatonin+luzindole (egzersizden önce intraperitonal 0,4 mg/kg dozda luzindol ve 10 mg/kg melatonin enjeksiyonu) grupları 5-25 m/ dk hızda koşu bandında yoruluncaya kadar koşturuldu. Egzersiz sonrasında EKG verileri kaydedildi. Bulgular: EKG verilerine göre, her iki egzersiz grubunda kalp hızının anlamlı düzeyde arttığı belirlendi. Melatonin uygulamasının egzersiz grubunda anlamlı düzeyde artan QT ve QTc süresi uzamalarını kısalttığı, luzindol verilen grupta ise melatoninin bu etkisinin ortaya çıkmadığı belirlendi. Melatonin aynı zamanda egzersizle artan kortikosteron seviyelerini reseptör bağımsız bir şekilde azalttı. Egzersizle artan akyuvar sayısı ve nötrofil düzeyleri melatonin grubunda kontrol grubuna yakın düzeyde belirlendi. Sonuç: Bu sonuçlar melatoninin, egzersizde ortaya çıkabilecek istenmeyen etkileri azaltıcı ve egzersiz kalitesini artırıcı bir ajan olarak kullanılabileceğini göstermektedir

The Investigation of the Effects of Melatonin on Electrocardiografic Findings in Rats Undergoing Acute Intense Exercise

Aim: Acute intense exercise causes an additional strain on cardiovascular system. Melatonin has been known as a hormone with cardiovascular protective effects. The aim of this study is to investigate the protective effects of melatonin on cardiovascular changes in rats undergoing acute intense exercise, considering electrocardiogram (ECG) results. Materials and Methods: The 3-5-month-old 28 Wistar albino rats were used in this study and they were divided into 4 groups. The vehicle injection was applied in the control group without any exercise procedure. The groups of exercise, exercise+melatonin (intraperitoneal injection of melatonin at the dose of 10 mg/kg before exercise) and exercise+melatonin+luzindole (intraperitoneal injection of luzindole at the dose of 0.4 mg/kg and melatonin at the dose of 10 mg/kg before exercise) were run on treadmill until they were tired. ECG recording was performed at the end of the exercise. Results: A significant increase in heart rate was observed in both exercise groups. The decreasing effect of melatonin on QT and QTc prolongation in the exercise group was reported; however, this effect did not occur in the luzindol administrated group. Melatonin also decreased corticositerone levels, which increased with exercise, independent of receptor. White blood cell and neutrophile counts of the melatonin administrated group were observed to be close to that of the control group.Conclusion: These results indicate that melatonin can be used as an agent that may decrease unfavorable effects of exercise and improve the quality of exercise.

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