FENOL TAYINI İÇIN ELMA DOKUSU VE ASITLE MUAMELE EDILMIŞ ÇOK DUVARLI KARBON NANOTÜP TEMELLI AMPEROMETRIK BIYOSENSÖR GELIŞTIRILMESI

Bu çalışmada, fenol tayini için asit fonksiyonelleştirilmiş çok duvarlı karbon nanotüp (f-ÇDKNT) ile kombinlenmiş elma dokusu temelli amperometrik biyosensörün çapraz bağlayıcı kullanılmayan bir yaklaşımla üretimi sunulmuştur. fÇDKNT-camsı karbon pasta kompoziti içinde hapsedilmiş elma dokusu doğal polifenol oksidaz kaynağı olarak fenolün enzimatik yükseltgenmesi için kullanılmış ve çözünmüş oksijenin tüketimi biyosensör yanıtı olarak kronoamperometri ile izlenmiştir. Deneysel parametrelerin (çalışma potansiyeli, pH ve doku miktarı) etkileri optimum ölçüm koşullarının elde edilmesi için incelenmiştir. Optimum koşullarda, amperometrik yanıtlar 10-200 µM fenol aralığında doğrusal olarak artmış ve gözlenebilme sınırı 3.26 µM olarak hesaplanmıştır (n=3). Elma dokusu temelli biyosensör, musluk suyu örneklerindeki fenolün tatmin edici geri kazanım değerleriyle tayini için kullanılmıştır.

DEVELOPMENT OF APPLE TISSUE AND ACID TREATED MULTI WALLED CARBON NANOTUBE BASED AMPEROMETRIC BIOSENSOR FOR PHENOL DETECTION

The fabrication of acid functionalized multi-walled carbon nanotube (fMWCNT) combined apple tissue based amperometric biosensor through a cross-linking agent-free approach has been presented for the phenol detection in this study. Apple tissue entrapped in fMWCNT-glassy carbon paste composite was employed as the natural polyphenol oxidase source for the enzymatic oxidation of phenol and the consumption of the dissolved oxygen was monitored via chronoamperometry as the biosensor response. The effect of experimental parameters (e.g. working potential, pH and tissue amount) were examined to obtain the optimum measurement conditions. Under optimized conditions, amperometric responses linearly increased in the range of 10-200 µM phenol and limit of detection was calculated as 3.26 µM (n=3). Apple tissue based biosensor was utilized for the phenol detection in tap water samples by serving satisfying recovery values.

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