Capsicum boccatum x eximium ve C. baccatum cardenasii F1 Hibritleri Üzerinde Polen Boyanabilirliliği ve Mayoz Calışmaları

Capsicum baccatum x C. eximium ve C. baccatum x C. cardenasii arasında yapılan melezlemeler sonucu elde edilen Fİ hibridler üzerinde mayoz ve polen boyaması çalışmaları yapılmıştır. Kromozomların birbirine yapışık durmasından ve bu yüzden iyi aynştınlamanıasından dolayı klasik ezme yöntemleriyle yapılan mayoz çalışmaları Capsicum'da iyi sonuç vermez. Bu yüzden diakinez veya metafaz aşamalarında yapılan klasik mayoz çalışmalarının yanısıra pakiten aşamasında synaptonemal kompleks çalışmalarının yapılmasına karar verilmiştir. Bu makalede yapılan çalışmalar süresince gözlenen problemler belirtilmiş ve bu problemlerin nedenleri açıklanmaya çalışılmıştır.

Meiotic Studies and Pollen Stainabilities of FI Hybrids Between Capsicum baccatum x C. eximium and C. baccatum x C. cardenasii

Meiotic studies have been conducted on F 1 hybrids between Capsicum baccatum x C. cardenasii and C, baccatum x C. eximium. Meiotic studies with conventional squashes in Capsicum are sometime troublesome, since ehromosomes can be sticky so that it is dfficult to get a good separation of ehrotnosomes, It was therefore decided to study synaptonemal complexes at pachytene, as well as conventional squashes at diakinesis or metaphase. In this article, problems observed during these kind of studies were noted and possible explanations were given.

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  • 1. Pickersgill, B., Relationships between weedy and cultivated forms in some species of chili peppers, (genus Capsicum). Evolution 25, 683-691, 1971.
  • 2. Pickersgill, B., Chromosemes and evolution in Capsicum 77. Edited by E. Pochard. I.N.R.A., Montfavet-Avignon, France, 1977.
  • 3. Koompai, P., Some barriers to interspecific crossing and gene exchange in five species of Capsicum. M.Phil. thesis, University of Reading, 1976.
  • 4. Tanksley, S.D., Linkage relationsips and chromosomal locations of enzyme coding genes in pepper, Capsicum ammm. Chromosoma, 89, 352-360, 1984.
  • 5. Haji itam, K., Studies on the relationship and barriers to hybridisation between the purple flowered C. cardenasii and white flowered C baccalum. M.Phil thesis, University of Reading, 1988.
  • 6. Gonzalez de Leon, D.R., Interspecific hybridisation and the cytogenetic architecture of two species of chili pepper (Capsicum-Solanaceae). Ph D. thesis, University of Reading, 1986.
  • 7. Burnham, C.R., Chromosomal interchanges in plants. Bot. Rev., 41, 49-62, 1956.
  • 8. Pickersgill, B., Cytogenetics and evolution of Capsicum. In; Chromosome engineering in plants; Genetics, Breeding, Evolution (Eds. T. Ttsuchiya and P.K. Gupta) Part B. pp. 139-159, 1991.
  • 9. Burnham, C.R. Discussion in cytogenetics. Burgess Publ. Co., Minneapolis, 1962.
  • 10. Snow, R., Alcoholic hydrochloric acid-carmine as stain for chromosomes in squash preparations. Stain Technol. 38, 9-13, 1963.
  • 11. Stack, S., Two dimensional spreads of synaptonemal complexes from Solanaceous plants. L The technique. Stain Technol. 57,265-272, 1982.
Mediterranean Agricultural Sciences-Cover
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1988
  • Yayıncı: Akdeniz Üniversitesi