The effect of mesenchymal stem cell-lyophilizate on the proliferation of A549 cells by regulation of JAK-STAT3 signaling pathway

To examine the effects of Mesenchymal Stem Cell-Lyophilizate (MSC-L) on the proliferation of A549 lung cancer cells and the activities of the JAK-STAT3 signaling pathway in this process. Our study in experimental design utilized A549 lung adenocarcinoma cells as model cells. First, the proliferative effect of the MSC-L cells on the A549 cells was established. Then, the concentration at which the proliferation was most distinct and a lower dilution were selected as the experimental group. In experimental groups, the A549 cells were exposed to the umbilical cord-derived MSC-L cells at two concentrations: 5X105 MSC-L cells/well and 20X105 MSC-L cells/well. In the control group, A549 cells with no exposure (only serum-free DMEM F12 with lyophilization additive used in lyophilization process of MSC-L cells) were used and the proliferation process of all three groups was monitored. The change in the expression levels of p53, c-MYC, p21, and SOCS2 did not statistically significantly different between the experimental group exposed to 5X105 MSC-L cells/well and the control group (p>0.05). On the other hand, the expression level of the Bcl-xL gene was statistically higher than in the control group (p=0.037). The expression level of the P21 gene was statistically higher at the concentration of 20X105 MSC-L cells/well compared to the control group (p=0.037). Similarly, the expression level of the Bcl-xL gene was statistically higher in the experimental group exposed to 20X105 MSC-L cells/well than in the control group (p=0.034). There was no statistically significantly different change in the expression levels of P53, c-MYC, and SOCS2 genes compared to the control group (p>0.05). Our study revealed that A549 cells exhibited an increased cell proliferation upon MSC-L exposure; however, the JAK-STAT3 pathway was not involved in the A549 cell proliferation, suggesting that there might be other signaling pathways involved.

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