Nilufer CELİK, Nursel B. OZTEKİN, Onder CELİK, Halil İbrahim OZEROL

Serum and Amniotic Fluid Eosinophil Cationic Protein Levels in Misoprostol Induced Pregnancies

The aim of this study was to determine the concentration of eosinophil cationic protein (ECP) in maternal serum (MS) and amniotic fluid (AF) at term and assess misoprostol induced changes in their mean concentrations. Methods: A total of 53 pregnant women were included in the study: 24 were in active labor, and a further 29 were not in labor and misoprostol induction was performed. The initial 3 mL of amniotic fluid was collected and processed. In all pregnant subjects, maternal blood samples were obtained from the cubital vein at the time of amniotic fluid sampling. The concentration of ECP in serum and AF was measured by chemiluminescence method with the immulite 2000 analyzer (Immulite2000® ECP, DPC Diagnostic Products Corporation 5700 West 96th Street Los Angeles, CA, USA). Results: Misoprostol induced pregnant showed significantly higher concentrations ECP than the control pregnant women in serum. AF-ECP concentrations were not significantly different between two groups. There was no significant difference between MS and AF-ECP concentrations in control subjects. Conclusion: The association we encountered between labor induction with misoprostol and increased systemic production of ECP should be investigated further.

Anahtar Kelimeler:

COPD, VDBP, genetic polymorphism, allele frequency

Serum and Amniotic Fluid Eosinophil Cationic Protein Levels in Misoprostol Induced Pregnancies

Keywords:

COPD, VDBP, genetic polymorphism, allele frequency,

PDF

___

Venge P, Bystrom J. Eosinophil cationic protein (ECP). Int J Biochem Cell Biol. 1998;30:433-7.
Junqueira LC, Zugaib M, Montes GS, Toledo OM, Krisztan RM, Shigihara KM. Morphologic and histochemical evidence for the occurrence of collagenolysis and for the role of neutrophilic polymorphonuclear leukocytes during cervical dilation. Am J Obstet Gynecol. 1980;138:273-81.
Kelly RW. Inflammatory mediators and cervical ripening. J Reprod Immunol. 2002;57:217-24.
Danforth DN. The fibrous nature of the human cervix, and its relation to the isthmic segment in gravid and nongravid uteri. Am J Obstet Gynecol. 1947;53: 541-60.
Menzies D, Nair A, Lipworth BJ. Non-invasive measurement of airway inflammation in asthma. J Asthma. 2006;43:407-15.
Wing DA. Labor induction with misoprostol. Am J Obstet Gynecol. 1999;181:3394
Cunningham FG, Gant NF, Leveno KJ, Gilstrap LC, Hauth JC, Wenstrom KD, eds, Williams’ obstetrics. 23rd ed, New York: McGraw-Hill; 2010;251-90.
Elati A, Weeks AD. The use of misoprostol in obstetrics and gynaecology. BJOG. 2009;1:61-9.
Weeks A, Alfirevic Z, Faşndes A, Hofmeyr GJ, Safar P, Wing D. Misoprostol for induction of labor with a live fetus. Int J Gynaecol Obstet. 2007;2:194-7.
Sennström MB, Ekman G, Westergren-Thorsson G, Malmström A, Byström B, Endrésen U, Mlambo N, Norman M, Ståbi B, Brauner A. Human cervical ripening, an inflammatory process mediated by cytokines. Mol Hum Reprod. 2000;6:375-81. Ledingham, M.A., Denison, F.C., Riley, S.C. et al. Matrix metalloproteinases –2 and –9 and their inhibitors (TIMPs) are produced by the human uterine cervix but their secretion is not regulated by nitric oxide donors. Hum Reprod. 1999;14:2089–96. Simpson JL, Scott RJ, Boyle MJ, Gibson PG. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma. Am J Respir Crit Care Med. 2005;172:559
Woerly G, Roger N, Loiseau S, Capron M. Expression of Th1 and Th2 immunoregulatory cytokines by human eosinophils. Int Arch Allergy Immunol. 1999;118:95-7.
Luque EH, Montes GS. Progesterone promotes a massive infiltration of the rat uterine cervix by the eosinophilic polymor-phonuclear leukocytes. Anat Rec. 1989;223:257
Duchesne M J, Badia E. Immunohistochemical localization of the eosinophil major basic protein in the uterus horn and cervix of the rat at term and after parturition. Cell Tissue Res. 1992;270:79-86.
Hegele-Hartung C, Chwalisz K, Beier HM, Elger W. Ripening of the uterine cervix of the guinea-pig after treatment with the progesterone antagonist onapristone (ZK 299): an electron microscopic study. Hum Reprod. 1989;4:369-77.
Knudsen UB, Uldbjerg N, Rechberger T, Fredens K. Eosinophils in human cervical ripening. Eur J Obstet Gynecol Reprod Biol. 1997;72:165-8.
Cantarini L, Volpi N, Carbotti P, Greco G, Aglianò M, Bellisai F, Giannini F, Alessandrini C, Grasso G, Galeazzi M. Eosinophilia-associated muscle disorders: an immunohistological study with tissue localisation of major basic protein in distinct clinicopathological forms. J Clin Pathol. 2009;62:442-7.
Wehrend A, Gümbel B, Leiser R, Failing K, Bostedt H. Vaginal and cervical dilatation intrapartum is associated with eosinophilic infiltration in the cow but not in the sheep. Reprod Domest Anim. 2004;39:442-6.
Kulkarni NS, Hollins F, Sutcliffe A, Saunders R, Shah S, Siddiqui S, Gupta S, Haldar P, Green R, Pavord I, Wardlaw A, Brightling CE. Eosinophil protein in airway macrophages: a novel biomarker of eosinophilic inflammation in patients with asthma. J Allergy Clin Immunol. 2010;126:61-9.
Xu X, Rivkind A, Pikarsky A, Pappo O, Bischoff SC, Levi-Schaffer F. Mast cells and eosinophils have a potential profibrogenic role in Crohn disease. Scand J Gastroenterol. 2004;39:440-7.
Osmers R, Adelmann-Grill BC, Fittkow C, Kuloczik M, Rath W, Szever6nyi M, Tschesche H, Kuhn W. Origin of cervical collagenase during parturition. Am J Obstet Gynecol. 1992;166:1455-60.
Tai P-C, Hayes DJ, Clark JB, Spry CJF. Toxic effects of human eosinophil secretion products on isolated rat heart cells in vitro. Biochem J. 1982;2:75-80.