Investigation of the use of seratec pmb test on postmortem peripheral blood samples for forensic purposes

Investigation of the use of seratec pmb test on postmortem peripheral blood samples for forensic purposes

The identification of the origin of biological stains at the crime scene is one of the important components of forensic science. In this study, it was aimed to evaluate the specificity performance of Seratec-PMB Test, which is used to differentiate menstrual blood from peripheral blood, on blood samples collected from dead bodies. Moreover, our purpose is to investigate the relationship between D-dimer accumulation in postmortem blood and other factors using this method. In this study, Seratec-PMB test is applied to peripheral blood samples of 200 dead bodies and the test results were compared in terms of parameters including age, gender, time between being found dead and sample collection, the cause of death. In Seratec-PMB Test, 71% of postmortem blood samples were positive and 29% were negative for D-dimer presence. A significant relationship was reported in the distribution of test results by age. SERATEC®-PMB test afforded a high rate of positive results in postmortem peripheral blood. It should be remembered that the test may give false positive results for postmortem peripheral blood. Since the test is a semi-quantitative test, it was understood that it would not allow any interpretation about the cause of death.

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  • 1. Holtkötter H, Dias Filho CR, et al. Forensic differentiation between peripheral and menstrual blood in cases of alleged sexual assault-validating an immunochromatographic multiplex assay for simultaneous detection of human hemoglobin and D-dimer. Int J Legal Med. 2018;132:683-90.
  • 2. Baker DJ, Grimes EA, Hopwood AJ. D-dimer assays for the identification of menstrual blood. Forensic Sci Int. 2011;212:210-4.
  • 3. Holtkötter H, Dierig L, Schürenkamp M, et al. Validation of an immunochromatographic D-dimer test to presumptively identify menstrual fluid in forensic exhibits. Int J Legal Med. 2015;129:37-41.
  • 4. Nesheim M. Thrombin and fibrinolysis. Chest. 2003;124(3 Suppl):33S-9S. 5. Cesarman-Maus G, Hajjar KA. Molecular mechanisms of fibrinolysis. Br J Haematol. 2005;129:307-21.
  • 6. Doolittle RF, Pandi L. Probing the beta-chain hole of fibrinogen with synthetic peptides that differ at their amino termini. Biochemistry. 2007;46:10033-8.
  • 7. Kanaide H, Shainof f JR. Cross-linking of fibrinogen and fibrin by fibrinstablizing factor (factor XIIIa). J Lab Clin Med. 1975 Apr;85:574-97.
  • 8. McKee PA, Mattock P, Hill RL. Subunit structure of human fibrinogen, soluble fibrin, and cross-linked insoluble fibrin. Proc Natl Acad Sci U S A. 1970;66:738-44.
  • 9. Siebenlist KR, Meh DA, Mosesson MW. Protransglutaminase (factor XIII) mediated crosslinking of fibrinogen and fibrin. Thromb Haemost. 2001;86:1221-8.
  • 10. Mosesson MW, Siebenlist KR, Hainfeld JF, Wall JS. The covalent structure of factor XIIIa crosslinked fibrinogen fibrils. J Struct Biol. 1995;115:88- 101.
  • 11. Miyaishi S, Kitao T, Yamamoto Y, et al. Identification of menstrual blood by the simultaneous determination of FDP-D dimer and myoglobin contents. Nihon Hoigaku Zasshi. 1996;50:400-3.
  • 12. Takeichi S, Wakasugi C, Shikata I. Fluidity of cadaveric blood after sudden death: Part I. Postmortem fibrinolysis and plasma catecholamine level. Am J Forensic Med Pathol. 1984;5:223-7.
  • 13. Sakurada K, Sakai I, Sekiguchi K, et al. Usefulness of a latex agglutination assay for FDP D-dimer to demonstrate the presence of postmortem blood. Int J Legal Med. 2005;119:167-71.
  • 14. Hernández-Cueto C, Vieira DN, Girela E, Marques E, Villanueva E, Sá FO. Diagnostic ability of D-dimer in the establishment of the vitality of wounds. Forensic Sci Int. 1995;76:141-9.
  • 15. Donaldson AE, Lamont IL. Biochemistry changes that occur after death: potential markers for determinin g post-mortem interval. PLoS One. 2013;8:e82011.
  • 16. SERATEC® PMB user instruction. 2019 https://www.seratec.com/docs/ user_instructions/2019/IFU_PMB_EN_2019-06.pdf
  • 17. Wang H. Evaluation of D-dimer in postmortem blood using the SERATEC PMB Test. Thesis, Boston University, 2019.
  • 18. Tita-Nwa F, Bos A, Adjei A, et al. Correlates of D-dimer in older persons. Aging Clin Exp Res. 2010;22:20-3.
  • 19. Bagwe K. Evaluation of recently developed methods for the forensic detection of menstrual blood. Thesis, Boston University, 2018.
  • 20. French CE, Jensen CG, Vintiner SK, et al. A novel histological technique for distinguishing between epithelial cells in forensic casework. Forensic Sci Int. 2008;178:1-6.
  • 21. Righini M, Le Gal G, De Lucia S, et al. Clinical usefulness of D-dimer testing in cancer patients with suspected pulmonary embolism. Thromb Haemost. 2006;95:715-9.
  • 22. Miron MJ, Perrier A, Bounameaux H, de Moerloose P, Slosman DO, Didier D, Junod A. Contribution of noninvasive evaluation to the diagnosis of pulmonary embolism in hospitalized patients. Eur Respir J. 1999;13:1365- 70.
  • 23. Chabloz P, Reber G, Boehlen F, et al. TAFI antigen and D-dimer levels during normal pregnancy and at delivery. Br J Haematol. 2001;115:150-2.
  • 24. Lip GY, Lowe GD. Fibrin D-dimer: a useful clinical marker of thrombogenesis? Clin Sci (Lond). 1995;89:205-14.
Medicine Science-Cover
  • ISSN: 2147-0634
  • Yayın Aralığı: 4
  • Başlangıç: 2012
  • Yayıncı: Effect Publishing Agency ( EPA )
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