Dosay Mine AKBULUT, Ayhan VURMAZ

Determination of experimentally given homocysteine causes to alzheimer-like dementia in rats on the basis of different parameters

Alzheimer’s disease (AD) is the most seen form of neurodegenerative disease with specific pathological findings like; senile plaques (CPs), synaptic loss, neurofibrillary tangles (NFTs) and neurodegeneration. Homocysteine is a naturally occurring amino acid and have a role on the body’s methylation process. In this study, total 12 rats, were used and divided into 2 groups. Group 1 Control group (n = 6): No rats were treated. Group 2 Application of High-Dose Homocysteine (n = 6): For 5 weeks, 4 mg / kg / day; to create an experimental Alzheimer model. Morris Water Maze test, the MDA, homocysteine (Hcy), reduced glutathione (GSH) and oxidised glutathione (GSSG) levels in rat liver, serum and erythrocyte samples were studied. Mitogen activated protein kinase (MAPK) and beta-secretase (BACE1) mRNA levels and pathological examinations were performed in rat hippocampus samples. The reaching time to exit platform was longer in the Hcy group according to Morris Water Maze test. Neurodegenerative areas were observed in the hippocampus CA1 region of Hcy group. Liver and serum Hcy and MDA levels and liver and erythrocyte GSSG levels were significantly higher (p

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1. Fargo K, Bleıler L. Alzheimer’ Association Report 2014 Alzheimer’s disease facts and figures. Alzheimers Dement. 2014;10:47-92.

2. Ferreira D, Perestelo-pérez L, Westman E, et al. Meta-review of csf core biomarkers in alzheimer’s disease: the state-of-the-art after the new revised diagnostic criteria. Front Aging Neurosci. 2014;6:47.

3. Kidd PM. Alzheimer’s disease, amnestic mild cognitive ımpairment, and age-associated memory ımpairment: current understanding and progress toward ıntegrative prevention. Altern Med Rev. 2008;13:85-115.

4. Chaı G-S, Jıang X, Feı Nı Z, et al. Betaine attenuates Alzheimer-like pathological changes and memory deficits induced by homocysteine. J Neurochem. 2013;124:388-96.

5. Brosnan JT, Brosnan ME. The sulfur-containing amino acids: an overview. J Nutr. 2006;136:1636-40.

6. Zhang Y, Zhu T, Wang L, et al. Homocysteine homeostasis and betaine-homocysteine s-methyltransferase expression in the brain of hibernating bats. PLoS ONE. 2013 8:e85632.

7. Cacciapuoti F. Lowering homocysteine levels with folic acid and B-vitamins do not reduce early atherosclerosis, but could interfere with cognitive decline and Alzheimer’s disease. J Thromb Thrombolysis. 2013;36:258–62.

8. Zhuo JM, Wang H, Praticò D. Is hyperhomocysteinemia an Alzheimer’s disease (AD) risk factor, an AD marker, or neither? Trends Pharmacol Sci. 2011;32:562-571.

9. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:e45.

10. Arslan S, Ateş E, Atikcan DT, ve ark. Stres altında olan sıçanlarda ve normal koşullardaki sıçanlarda antidepresan “fluoksetin hidroklorür kullanımının öğrenme üzerine etkileri. :http://tip.baskent.edu.tr/egitim/mezuniyetoncesi/calismagrp/ogrsmpzsnm13/13.S14.pdf] erişim tarihi 2013.

11. Li MH, Tang JP, Zhang P, et al. Disturbance of endogenous hydrogen sulfide generation and endoplasmic reticulum stress in hippocampus are involved in homocysteine-induced defect in learning and memory of rats. Behav Brain Res. 2014;262:35-41.

12. Zylberstein DE, Lissner L, Björkelund C, et al. Midlife homocysteine and late-life dementia in women. A prospective population study. Neurobiol Aging. 2011;32:380-6.

13. Farkas M, Keskitalo S, Smith DE, et al. Hyperhomocysteinemia in Alzheimer’s disease: the hen and the egg? J Alzheimers Dis. 2013;33:1097-104.

14. Fuso A, Nicolia V, Ricceri L, et al. S-adenosylmethionine reduces the progress of the Alzheimer-like features induced by B-vitamin deficiency in mice. Neurobiol Aging. 2012;33:1482.e1-16.

15. Morris MS1. Homocysteine and Alzheimer’s disease. Lancet Neurol. 2003;2:425-8.

16. Nazef K, Khelil M, Chelouti H, et al. Hyperhomocysteinemia ıs a risk factor for Alzheimer’s disease in an algerian population. Arch Med Res. 2014;45:247-50.

17. Guo J, Ni S, Li Q, et al. Folate/Vitamin B Alleviates hyperhomocysteinemia-ınduced alzheimer-like pathologies in rat retina. Neurosci Bull. 2019;35:325-35.

18. Kovalska M, Tothova B, Kovalska L, et al. Association of ınduced hyperhomocysteinemia with alzheimer’s disease-like neurodegeneration in rat cortical neurons after global ıschemia-reperfusion ınjury. Neurochem Res. 2018;43:1766-78.

19. Mahaman Y, Huang F, Wu M, et al. Moringa oleifera alleviates homocysteine-ınduced Alzheimer’s disease-like pathology and cognitive ımpairments. J Alzheimers Dis. 2018;63:1141-59.

20. Singh M, Prakash A. Possible role of endothelin receptor against hyperhomocysteinemia and β-amyloid induced AD type of vascular dementia in rats. Brain Res Bull. 2017;133:31-41.