Protective effects of curcumin on liver damage in rats treated with chlorpyrifos during pregnancy

The purpose of this study is to examine the damage of chlorpyrifos, which is a type of pesticide commonly used in agriculture, will cause in the livers of rats during the gestation period and to examine to what extent and in which direction curcumin, an antioxidant, influences this damage. For this purpose, four groups of animals as the control, chlorpyrifos, curcumin, and chlorpyrifos+curcumin were used in the study. During their pregnancy, curcumin (100mg/kg) and chlorpyrifos (5 mg/kg) were given to rats (Wistar Albino) through lavage. After the birth, perfusion was performed to rats under deep anesthesia, and their livers were removed. After the livers were fixated with 10% buffered neutralized formalin, routine histological processes were performed All the tissues were embedded in paraffin and the tissue sections obtained were stained with hematoxylin-eosin, periodical acid-Shiff (PAS) and Masson ’s trichrome staining. According to the results of the study, with the effect of chlorpyrifos, vesicular degeneration in the livers, enlargement, inflammation centers and necrotic cells in the sinusoids were observed. Also, especially the increase in the collagen fiber amount around the portal areas and the decrease in glycogen storages around the central veins were remarkable. In the groups, which were given only curcumin, no obvious changes were seen in the liver except for mild enlargements in the sinusoids. In groups, which were given chlorpyrifos and curcumin together, less histopathological changes were seen when compared with groups, which were given only chlorpyrifos. With these results obtained, it can be said that curcumin, which is a free radical scavenger decreases the oxidative damage of chlorpyrifos in the liver by inducing its free radical formation.

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1. Vural N: Toksikoloji. , Ankara, A.Ü. Basımevi, 1984.

2. Ekebaş S, Çakır S, Ertuğrul O, Kence A. The detection of mutagenic activity of some chemicals (azamethypos, dichlorvos, methyl parathion, aflatoxin b1) by the smart test in drosophila melanogaster. Turk. J. Vet. Anim. Sci. 2000;24:563-9.

3. Reuber MD. Carcinomas and other lesions of the liver in mice ingesting organochlorine pesticides. Clinical Toxicol.1978;13:231-56,

4. Eaton DL, Daroff RB, Autrup H, et al. Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol. 2008;38:1-125.

5. Deveci HA, Karapehlivan M, Kaya İ, et al. Akut klorprifos-etil zehirlenmesine karşı kafeik asit fenetil ester’in koruyucu etkisi, Ankara Üniv Vet Fak Derg. 2015;62:255-60.

6. Lemaire P, Matthews A, FörlinL, et al. Stimulation of oxyradical production of hepatic microsomes of flounder (platichysflesus) and perch (percafluviatilis) by model and pollutant xenobiotics. Arch Environ Contam Toxicol ,1994;26:191-200.

7. Kurutaş EB, Kılınç M. Pestisitlerin Biyolojik Sistemler Üzerine Etkisi, Arşiv, 2003;12:215.

8. Alp, H, Sak, ME, Evsen MS, et al. Effects of malathion in fetal kidney tissues in pregnant rats: teratogenic effects induced by different doses. Kafkas Univ Vet Fak Derg. 2012;18:221-6.

9. Bagchi D, Bagchi M, Hassoun EA, et al. In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology. 1995;104:129-40.

10. Lodovici M, Cassalini C, Briani C, et al. Oxidative liver DNA damage in rats treated with pesticide mixtures. Toxicology. 1997;117:55-60.

11. Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: evidence of its antioxidant property. Exp Toxicol Pathol. 2011;63:419-31.

12. Sreejayan RMN. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol. 1997;49:105-7.

13. Thiyagarajan M, Sharma SS. Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats. Life Sci. 2004;74:969-85.

14. Mique IJ, Bernd A, Semperen JM, et al. The curcuma antioxidants: pharmacological effects and prospects for future clinical use. Arch Gerontol Geriatr. 2002;34:37-46.

15. Soma-Pillay P, Nelson-Piercy C, Tolppanen H, et al. Physiological changes in pregnancy. Cardiovasc J Afr. 2016;27:89-94.

16. AFIP, Laboratory Methods in Histotechnology. 1992;132:53-8.

17. Karaduman D, Eren B, Keles ON. The protective effect of beta-1,3-D-glucan on taxol-induced hepatotoxicity: a histopathological and stereological study. Drug Chem Toxicol. 2010;33:8-16.

18. Gülcü F, Gürsu MF. Paraoksonaz ve arilesteraz aktivite ölçümlerinin standardizasyonu. Turk J. Biochem. 2002;8:45-9.

19. Uzun FG, Demir F, Kalender S, et al. Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food Chem Toxicol. 2010;48:1714-20.

20. Ambali FS, Ayo JO, Kan E, et al. Hemotoxicity induced by chronic chlorpyrifos exposure in wistar rats: mitigating effect of vitamin C. Vet Med Int. 2011.

21. Tripathi S, Srivastav AK. Liver profile of rats after long-term ingestion of different doses of chlorpyrifos Pestic Biochem Physiol. 2010;97:60-5.

22. Uzun, FG, Kalender, Y. Chlorpyrifos induced hepatotoxic and hematologic changes in rats: The role of quercetin and catechin. Food Chem Toxicol. 2013;55:549-56.

23. Mansour SA, Mossa ATH. Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc. Pestic Biochem Physiol. 2010:96:14-23.

24. Goel A, Dani V, Dhawan DK. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity Chem Biol Interact. 2005;156:131-40.

25. Nishi K, Hundal SS. Chlorpyrıfos ınduced hıstologıcal and bıochemıcal changes ın the lıver and kıdney tıssues of female Wıstar rats. Intern J Advanced Res. 2015;3:1190-6.

26. Saraı A, Osorio J, Monroy A, et al. International Union of Biochemistry and Molecular Biology. 2016;42:561-80.

27. Seki E, Schwabe RF. Hepatic inflammation and fibrosis: functional links and keypathways. Hepatology, 2015;61:1066-79.

28. Friedman SL. Mechanisms of hepatic fibrogenesis. Gastroenterology. 2008;134:1655-69.

29. Wani H, Rehman S, Shoukat S, et al. A study on chlorpyrifos induced oxidative stress in broiler chickens. Intern J Live Stock Res. 2017;7:3.

30. Georgiadisa G, Mavridisa C, Belantisa C, et al. Mamoulakisa Toxicology Nephrotoxicity issues of organophosphates. 2018;406-7.

31. Leek S, Buck M, Houglum K, et al. Activation of hepatic stellate cells by TGFȕ and collagen type I is mediated by oxidative stress through c-myb expression. J Clin Investigat. 1995;96:2461-68.

32. Bruck R, Ashkenazi M, Weiss S, et al. Prevention of liver cirrhosis in rats by curcumin. Liver Int. 2007;27:373-83.

33. Connelland MAO, Rushworth SA. Curcumin: potential for hepatic fibrosis therapy? British J Pharmacol. 2008;153:403-5.

34. Akgün N. Maternal beden kütle indeksi ve gebelikte vücut ağırlığı artışı takibinin perinatal sonuçlar ile ilişkisi. Hacettepe Üniversitesi Sağlık Bilimleri Enstitüsü, Beslenme Bilimleri Programı, Yüksek Lisans Tezi Ankara, 2013

35. Silvaa JG, Boaretob AC, Schreiberb AK, et al. Chlorpyrifos induces anxiety-like behavior in offspring rats exposed during pregnancy Neuroscience Letters. 2017;641:94-100.

36. Ishida T, Taketoh J, Nakatsune E, et al. Curcumin anticipates the suppressed body weight gain with 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. Jof Health Sci. 2004;50:474-82.

37. Ciftci O, Tanyildizi S, Godekmerdan A. Protective effect of curcumin on immune system and body weight gain on rats intoxicated with 2,3,7,8-Tetrachlorodi benzo-p-dioxin (TCDD) Immunopharmacol Immunotoxicol. 2010;32:99-104.

38. Venkatesan N, Punithavathi D, Arumugam V. Curcumin prevents adriamycin nephrotoxicity in rats. Br J Pharmacol. 2000;129:231-4.