Antitumor Activity of Propolis on Differantiated Cancer Cell Lines
Antitumor Activity of Propolis on Differantiated Cancer Cell Lines
Propolis is a natural bee product with several pharmacological activities. Nowadays, it is also investigated for its antitumor properties. There are contraversies on the antitumor activity of propolis, not all tumour cells seem to respond to propolis treatment. The aim of our study is to evaluate the activity of propolis on differantiated thyroid cancer cell lines. Tyripan blue test and MTT assay were performed to evaluate the cell viability of B-CPAP cells after propolis treatment and compared with propolis free cells and normal thyroid cells. Evaluation with light microscopy revealed after treatment with propolis, cell count decreased and B-CPAP cells displayed morphologic changes with a smaller appearance. Tyripan blue exclusion test showed a decrease in cell viability in different concentrations of propolis at 48 and 72 h. Cell viability was measured by MTT after the treatment of B-CPAP cells with propolis at 72 h. The decrease in cell viability was statistically significant only at 0.0001 diluted propolis (p=0.048). The decrease in cell viability in normal thyroid cell line was not statistically significant. In conclusion, propolis have weak antitumor activity on differantiated thyroid cell line which may contribute to different degrees of sensitivity of propolis among cancer cells.
___
- Marcucci MC: propolis: chemical composition, biological properties and therapeutic activitiy. Apidologie. 1995;26:88-99.
- Sawicka D, Car H, Borawska MH, Niklinski J. The anticancer activity of orpoplis. Folia Histochemica et cytobiologica 2012; 50(1): 25-37.
- Aso K, Kanno S, Tadano T, Satoh S, Ishikawa M. Inhibitory effect of propolis on the growth of human leukemic U937. Biol Pharm Bull. 2004;27:727–30.
- Orsolic N, Basic I. Immunomodulation by water-soluble derivative of propolis: a factor of antitumor reactivity. J Ethnopharmacol. 2003;84:265–73.
- Orsolic N, Knezevic AH, Sver L, Terzic S, Basic I. Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds. J Ethnopharmacol. 2004;94:307–15.
- Vatansever SH, Sorkun K, Ismet Deliloğlu Gurhan S, Ozdal-Kurt F, Turkoz E, Gencay O, Salih B. Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines. Acta Histochem. 2010;112:546–56.
- Eom HS, Lee EJ, Yoon BS, Yoo BS. Propolis inhibits the proliferation of human leukaemia HL-60 cells by inducing apoptosis through the mitochondrial pathway. Nat Prod Res. 2010;24:375–86.
- Szliszka E, Czuba ZP, Domino M, Mazur B, Zydowicz G, Krol W. Ethanolic extract of propolis (EEP) enhances the apoptosis-inducing potential of TRAIL in cancer cells. Molecules. 2009;14:738–54.
- Jin UH, Song KH, Motomura M, Suzuki I, Gu YH, Kang YJ, Moon TC, Kim CH. Caffeic acid phenethyl ester induces mitochondria-mediated apoptosis in human myeloid leukemia U937 cells. Mol Cell Biochem. 2008;310:43–48.
- Watabe M, Hishikawa K, Takayanagi A, Shimizu N, Nakaki T. Caffeic acid phenethyl ester induces apoptosis by inhibition of NFkappaB and activation of Fas in human breast cancer MCF-7 cells. J Biol Chem. 2004;279:6017–26.
- Chen MJ, Chang WH, Lin CC, Liu CY, Wang TE, Chu CH, Shih SC, Chen YJ. Caffeic acid phenethyl ester induces apoptosis of human pancreatic cancer cells involving caspase and mitochondrial dysfunction. Pancreatology. 2008;8:566–76.
- Daugsch A, Moraes CS, Fort P, Park YK. Brazilian red propolis — chemical composition and botanical origin. Evid Based Complement Alternat Med. 2008;5:435–41.
- Nieva MMI, Isla MI, Cudmani NG, Vattuone MA, Sampietro AR. Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis. J Ethnopharmacol. 1999;68:97–102.
- Sibel S, Semiramis K. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol. 2005;99:69–73
- Uzel A, Sorkun K, Onçağ O, Cogŭlu D, Gençay O, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res. 2005;160:189–195.
- Gunduz C, Biray C, Kosova B, Yilmaz B, eroglu Z, Sahin F, Omay SB, Cogulu O. Evaluation of Manisa propolis effect on leukemia cell line by telomerase activity.Leukemia research 29. 2005; 29:1343-6.
- Barlak Y, Deger O, Colak M, Karataylı SC, Bozdayı M, Yücesan F: Effect of Turkish propolis extracts on proteome of prostate cancer cell line. Proteome Science. 2011;9:74.
- Nadia BH, Wided K, Kheira B Hassiba R, Lamia B, Rhouati S, Alyane M, Zellagui A, Lahouel M. Disruption of mitochondrial membrane potential by ferulenol and restoration by propolis extract: antiapoptotic role of propolis. Acta Biol Hung. 2009;60:385–98.