Mehmet BERKOZ

Antioxidant and anti-lipoxygenase activities of Cydonia oblonga

The present studies are focused to explore the antioxidant and anti-lipoxygnease activities in crude extract, aqueous and organic fractions (ethyl acetate, chloroform, butanol) of dry fruit of Cydonia oblonga. For determining the antioxidant activity; total phenolic content and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay were performed in the aqueous and organic fractions of C. oblonga. In our study, phenolic content revealed that crude extract has highest phenolic content followed by ethyl acetate fraction. The results of DPPH radical scavenging activity showed IC50 values of crude extract as 61.4 μg/mL, chloroform and aqueous 67.4 and 55.5 μg/mL, respectively, which are well comparable with butylatedhydroxyanisol (BHA) standard, which showed IC50 as 44.2 μg/mL. In addition, crude extract appeared to be the prominent inhibitor of lipoxygenase. The results suggests that the extract and different fractions of dry fruit of C. oblonga exhibits significant antioxidant and free radical scavenging activities/capacity which might be associated with the presence of main phytochemicals like flavonoids, phenols and tannins. Besides that, all these extract and fractions have well enough lipoxygenase inhibitory potential. As a result, we can claim that the presence of the scavenger molecules in the dry fruit of C. oblonga may attribute health benefits by providing the protective effects during the progressive stages of different acute and chronic disorders.

PDF

___

1. Fleschin S, Fleschin M, Nita S, et al. Free radical mediated protein oxidation in Biochemistry. Roun Biotechnol Lett. 2000;5:479-95.

2. Reddy VD, Padmavathi P, Paramahamsa M, et al. Amelioration of alcoholinduced oxidative stress by Emblicaofficinalis (Amla) in rats. Ind J Biochem Biophys. 2010;47:20-5.

3. Rani P, Khullar N. Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi. Phytother Res. 2004;18:670-3.

4. Ashraf MU, Muhammad G, Hussain MA, et al. Cydonia oblonga M., A medicinal plant rich in phytonutrients for pharmaceuticals. Front Pharmacol. 2016;7:163.

5. Erturk AG, Erturk O, Ayvaz MC, et al. Screening of phytochemical, antimicrobial and antioxidant activities in extracts of some fruits and vegetables consumed in Turkey. CBU J Sci. 2018;14:81-92.

6. Stojanović BT, Mitić SS, Stojanović GS, et al. Phenolic profiles and metal ions analyses of pulp and peel of fruits and seeds of quince (Cydonia oblonga Mill.). Food Chem. 2017;232:466-75.

7. Yaqeen Z, Naqvi NH, Sohail T, et al. Screening of solvent dependent antibacterial activity of Prunus Domestica. Pak J Pharm Sci. 2013;26:409-14.

8. Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondiaspinnata. BMC Complement Altern Med. 2008;8:63.

9. Iqbal L, Lateef L, Ali S, et al. Antioxidant activities of tetraketones derived from 5, 5 dimethylcyclohexane-1, 3- dione. J Chem Soc Pak. 2007;29:51-4.

10. Tappel AL. Method of Enzymology, Vol 5. Academic Press. New York, 1962, 539-42.

11. Ahmad M, Imran H, Yaqeen Z, et al. Pharmacological profile of Salvadora Persica. Pak J Pharm Sci. 2011;24:323-30.

12. Rehman A, Fatima N, Imran H, et al. Nutritional and toxicological evaluation of Grewiaasiatica (Phalsa) powder used as a summer drink. J Chem Soc Pak. 2013;35:320-3.

13. Wojdylo A, Oszmianski J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 2007;105:940-9.

14. Chen X, Sood S, Yang CS, et al. Five lipoxygenase pathway of arachidonic acid metabolism in carcinogenesis and cancer chemoprevention. Curr Cancer Drug Targets. 2006;6:613-22.