Ratlarda Oluşturulan Deneysel Periodontitis Modelinde Alveoler Kemik Kaybının Ölçümünde İki Morfometrik Doğrusal Yöntemin Karşılaştırılması

Amaç: Bu çalışmanın amacı, alveoler kemik kaybını değerlendirmek için direkt (alveoler kemik üzerinde dijital bir kumpas ve dental loop ile gerçekleştirilen) ve indirekt (dijital fotoğraflarda özel bir yazılım programı aracılığıyla gerçekleştirilen) doğrusal morfometrik ölçümleri karşılaştırmaktır. Gereç ve Yöntemler: Çalışmamızda kullanılan 30 adet Sprague Dawley sıçan alt çenesi ligatür modeli ile deneysel periodontitis oluşturulmuş önceki bir çalışmadan elde edilmiştir. Mine-sement sınırından alveoler kemik kretine olan mesafe, direkt ölçüm grubunda dijital kumpas ve periodontal sond kullanılarak dental loop ile ölçülürken; indirekt ölçüm grubunda ise özel bir görüntü analiz programı ile dijital fotoğraflar üzerinde gerçekleştirilmiştir. Bulgular: Mann-Whitney U testi, gruplar arası değerlendirme için kullanılmıştır. Alveoler kemik kaybı direkt ölçüm grubunda 1,08±0,22 mm; indirekt ölçüm grubunda ise 1,19±0,33 mm olarak hesaplanmışır. Gruplar arasında alveoler kemik kaybı açısından istatistiksel bakımdan anlamlı bir değişim belirlenmemiştir (p>0,05). Sonuç: Bu sonuca dayanarak, direkt morfometrik analiz kullanımının, sıçanlarda deneysel periodontitis oluşumundan sonra meydana gelen alveoler kemik kaybını ölçmek için indirekt morfometrik değerlendirmelere alternatif olabileceğini söyleyebiliriz.

Comparison of Two Morphometric Linear Methods to Measure the Alveolar Bone Loss Induced by Experimental Periodontal Disease in Rats

Objective: The aim of this study was to compare the direct and indirect linearmorphometric measurements for the evaluation of alveolar bone loss afterexperimentally induced periodontitis in rats.Materials and Methods: Right mandibles of 30 Sprague Dawley rats were collectedfrom a previous study which induced periodontitis using ligature model. In directmeasurement group, the distance between cementoenamel junction and alveolarbone crest was evaluated by digital caliper and periodontal probe with a dentalloop, whereas indirect group measurement was performed by an image analysisprogram on digital photographs.Results: The difference between groups was determined using Mann-Whitney Utest. Average alveolar bone loss was 1.08±0.22 mm in direct measurement groupand 1.19±0.33 mm inindirect measurement group. The variation between directand in direct groups was not statistically significant (p>0.05).Conclusion: Based on the present result, it can be concluded that, directmorphometric analysis might be an essential alternative to indirect morphometricevaluations to measure alveolar bone loss after experimentally induced periodontitisin rats.

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