Nazlı Gülriz ÇERİ, Kanat GÜLLE, Mehmet ARASLI, Meryem AKPOLAT, Buket DEMİRCİ

Protective Effect of Vitamin B5 (Dexpanthenol) on Nephropathy in Streptozotocin Diabetic Rats

Objective: This study aimed to evaluate the effects of vitamin B5 [dexpanthenol, DEX] treatment on the kidney in a streptozotocin (STZ) diabetes animal model. Materials and Methods: Twenty-four Wistar rats were randomised in one of the four groups: Group 1 served as control, and group 2 was administered high-dose DEX (300 mg/kg/day) as a safety group. Diabetes was induced by intraperitoneal injection of a single dose of STZ (50 mg/kg) in groups 3 and 4. In group 4, DEX was administered as well. All groups were followed up for 6 weeks. Histopathological analyses were performed on renal tissue using periodic acid-Schiff and HematoxylinEosin stains. Results: Microscopic evaluation of the kidney revealed that the rats demonstrated kidney damage 6 weeks after STZ administration. However, high-dose and longterm DEX administration alone did not damage renal tissue; moreover, kidney damage was prevented if DEX was administered in the early phase of diabetes. Conclusion: DEX could be a safe and cost-effective vitamin for the prevention of diabetes complications.

Sıçanlarda B5 Vitamininin (Dekspantenol) Nefropatiye Etkisi

Amaç: Çalışmamızda streptozotosin (STZ) diyabet hayvan modelinde B5 [dexpanthenol (DEX)] vitamini ile tedavinin böbrek üzerindeki etkilerini değerlendirdik. Gereç ve Yöntemler: Yirmi dört wistar sıçanı dört gruba eşit ve randomize olarak dağıtıldı. Grup 1 kontrol grubu; grup 2 güvenlik grubu olarak yüksek doz DEX (300 mg/kg/gün) verildi. Diyabet, grup 3 ve 4’te tek bir intraperitoneal doz STZ (50 mg/ kg) enjeksiyonu ile oluşturuldu. Grup 4 DEX ile tedavi edildi. Tüm gruplar 6 hafta takip edildi. Periyodik asit Schiff ve Hematoksilen-Eosin kullanılarak böbrek dokusu üzerinde histopatolojik incelemeler yapıldı. Bulgular: Böbreğin mikroskobik değerlendirmesinde, sıçanlarda STZ müdahalesinden 6 hafta sonra böbrek hasarı geliştiği görüldü. Tek başına yüksek ve uzun süreli DEX uygulaması böbrek dokusu için zararlı değildi. Ayrıca diyabetin erken evresinde DEX verilmesi böbrek hasarını önledi. Sonuç: Diyabet komplikasyonlarını önlemek için DEX güvenli ve uygun maliyetli bir vitamin olabilir.

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1. Tomino Y, Cooper ME, Kurtz TW, Shimizu Y. Experimental models of type-2 diabetic nephropathy. Exp Diabetes Res 2012; 2012: 218917.

2. Moon JY, Jeong KH, Lee TW, Ihm CG, Lim SJ, Lee SH. Aberrant recruitment and activation of T cells in diabetic nephropathy. Am J Nephrol 2012; 35: 164-74.

3. Wu CC, Chen JS, Lu KC, Chen CC, Lin SH, Chu P, et al. Aberrant cytokines/chemokines production correlate with proteinuria in patients with overt diabetic nephropathy. Clin Chim Acta 2010; 411: 700-4.

4. Hatano M, Hodges RE, Evans TC, Hagemann RF, Leeper DB, Bean WB, et al. Urinary excretion of pantothenic acid by diabetic patients and by alloxan-diabetic rats. Am J Clin Nutr 1967; 20: 960-7.

5. Lenzen S. The mechanisms of alloxan- and streptozotocininduced diabetes. Diabetologia 2008; 51: 216-26.

6. Depeint F, Bruce WR, Shangari N, Mehta R, O’Brien PJ. Mitochondrial function and toxicity: role of the B vitamin family on mitochondrial energy metabolism. Chem Biol Interact 2006; 163: 94-112.

7. Etensel B, Ozkisacik S, Ozkara E, Karul A, Oztan O, Yazici M, et al. Dexpanthenol attenuates lipid peroxidation and testicular damage at experimental ischemia and reperfusion injury. Pediatr Surg Int 2007; 23: 177-81.

8. Wojtczak L, Slyshenkov VS. Protection by pantothenic acid against apoptosis and cell damage by oxygen free radicals--the role of glutathione. Biofactors 2003; 17: 61-73.

9. Slyshenkov VS, Piwocka K, Sikora E, Wojtczak L. Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis. Free Radic Biol Med 2001; 30: 1303-10.

10. Halvorsen O, Skrede S. Regulation of the biosynthesis of CoA at the level of pantothenate kinase. Eur J Biochem 1982; 124: 211-5.

11. Slyshenkov VS, Dymkowska D, Wojtczak L. Pantothenic acid and pantothenol increase biosynthesis of glutathione by boosting cell energetics. FEBS Lett 2004; 569: 169-72.

12. Bayrak O, Seckiner I, Solakhan M, Karakok M, Erturhan SM, Yagci F. Effects of intravesical dexpanthenol use on lipid peroxidation and bladder histology in a chemical cystitis animal model. Urology 2012; 79: 1023-6.

13. Gulle K, Ceri NG, Akpolat M, Arasli M, Demirci B. The effects of dexpanthenol in streptozotocin-induced diabetic rats: histological, histochemical and immunological evidences. Histol Histopathol 2014; 29: 1305-13.

14. Demirci B, Demir O, Dost T, Birincioglu M. Protective effect of vitamin B5 (dexpanthenol) on cardiovascular damage induced by streptozocin in rats. Bratisl Lek Listy 2014; 115: 190-6.

15. Altintas R, Parlakpinar H, Beytur A, Vardi N, Polat A, Sagir M, et al. Protective effect of dexpanthenol on ischemia-reperfusioninduced renal injury in rats. Kidney Blood Press Res 2012; 36: 220-30.

16. Tutun B, Elbe H, Vardi N, Parlakpinar H, Polat A, Gunaltili M, et al. Dexpanthenol reduces diabetic nephropathy and renal oxidative stress in rats. Biotech Histochem 2019; 94: 84-91