Ozon Tedavisinin Karyojenik Bakteriler ÜzerindekiAntimikrobiyal Etkinliği

Amaç: Ozon tedavisinin karyojenik bakteriler üzerindeki antimikrobiyal etkinliğinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışma derin dentin çürüklü daimi birinci azı dişe sahip 40 çocuk üzerinde gerçekleştirilmiştir. Hastalar klorheksidin diglukonat glukonat (CHX) grubu ve ozon grubu olarak iki gruba ayrılmıştır. Daimi büyük azı dişlerden uygulama öncesi ve sonrası dentin çürüğü örnekleri toplanmıştır (120 sn ozon ile tedavi ve 60 sn %2 CHX çözeltisi uygulanmıştır). Kırk sekiz saat anaerob şartlarda etüvde bekletilen besiyerlerinde Streptococcus mutans (S. mutans) ve Lactobacilus kolonileri sayılmış ve değerler kaydedilmiştir. Elde edilen veriler istatistiksel olarak değerlendirilmiştir. Bulgular: Çalışmamızda elde edilen verilere göre, ozon ve CHX uygulamasının antimikrobiyal etkinliği S. mutans ve Lactobacilus’lar üzerinde istatistiksel açıdan anlamlı bulunmuştur (p

Antimicrobial Efficacy of Ozone Therapy on Cariogenic Bacteria

Objective: This study aimed to evaluate the antimicrobial effectiveness of ozone therapy on cariogenic bacteria. Materials and Methods: We evaluated 40 children with deep caries in the permanent first molar. Patients were divided into ozone and chlorhexidine digluconate (CHX) treatment groups. Cariogenic dentin samples were collected from permanent molars before and after treatment: 120 seconds with ozone and 60 s with 2% CHX solution. Streptococcus mutans (S. mutans) and Lactobacillus sp. colonies were counted after 48 hours of incubation in phosphate buffer. Obtained data were statistically analysed. Results: Significant differences between antimicrobial activities of ozone and CHX against S. mutans and Lactobacillus sp. were observed (p

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1. Pitts NB, Zero DT, Marsh PD, Ekstrand K, Weintraub JA, RamosGomez F, et al. Dental caries. Nat Rev Dis Primers 2017; 3: 17030.
2. Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and remineralization (part 1). J Clin Pediatr Dent 2003; 28: 47-52.
3. Lenander-Lumikari M, Loimaranta V. Saliva and dental caries. Adv Dent Res 2000; 14: 40-7.
4. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002; 15: 167-93.
5. Johansson E, Claesson R, van Dijken JW. Antibacterial effect of ozone on cariogenic bacterial species. J Dent 2009; 37: 449-53.
6. Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA. The role of sucrose in cariogenic dental biofilm formation--new insight. J Dent Res 2006; 85: 878-87.
7. Bernimoulin JP. Recent concepts in plaque formation. J Clin Periodontol 2003; 30(Suppl 5): 7-9.
8. Rickard AH, Gilbert P, High NJ, Kolenbrander PE, Handley PS. Bacterial coaggregation: an integral process in the development of multi-species biofilms. Trends Microbiol 2003; 11: 94-100.
9. Scheie AA. Mechanisms of dental plaque formation. Adv Dent Res 1994; 8: 246-53.
10. Thomas JG, Nakaishi LA. Managing the complexity of a dynamic biofilm. J Am Dent Assoc 2006; 137(Suppl): 10-5.
11. van Houte J, Lopman J, Kent R. The predominant cultivable flora of sound and carious human root surfaces. J Dent Res 1994; 73: 1727-34.
12. Zero DT. In situ caries models. Adv Dent Res 1995; 9: 214-30.
13. Brambilla E, García-Godoy F, Strohmenger L. Principles of diagnosis and treatment of high-caries-risk subjects. Dent Clin North Am 2000; 44: 507-40.
14. Newbrun E. Cariology. 3rd ed. Chicago: Quintessence Publishing Co; 1989: 389.
15. Ersin NK, Uzel A, Aykut A, Candan U, Eronat C. Inhibition of cultivable bacteria by chlorhexidine treatment of dentin lesions treated with the ART technique. Caries Res 2006; 40: 172-7.
16. Carrilho MR, Carvalho RM, Sousa EN, Nicolau J, Breschi L, Mazzoni A, et al. Substantivity of chlorhexidine to human dentin. Dent Mater 2010; 26: 779-85.
17. Arita M, Nagayoshi M, Fukuizumi T, Okinaga T, Masumi S, Morikawa M, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol 2005; 20: 206-10.
18. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol 2004; 19: 240-6.
19. Miranda C, Vieira Silva G, Damiani Vieira M, Silva Costa SX. Influence of the chlorhexidine application on adhesive interface stability: literature review. RSBO 2014; 11: 276-85.
20. McDonald RE, Avery DR, Dean JA. Dentistry for the child and adolescent. 8th ed. St Louis: Mosby, 2004: 769.
21. Bjørndal L, Mjör IA. Pulp-dentin biology in restorative dentistry. Part 4: Dental caries--characteristics of lesions and pulpal reactions. Quintessence Int 2001; 32: 717-36.
22. Kidd EA, Beighton D. Prediction of secondary caries around tooth-colored restorations: a clinical and microbiological study. J Dent Res 1996; 75: 1942-6.
23. Azrak B, Callaway A, Grundheber A, Stender E, Willershausen B. Comparison of the efficacy of chemomechanical caries removal (Carisolv) with that of conventional excavation in reducing the cariogenic flora. Int J Paediatr Dent 2004; 14: 182-91.
24. Lager A, Thornqvist E, Ericson D. Cultivatable bacteria in dentine after caries excavation using rose-bur or carisolv. Caries Res 2003; 37: 206-11.
25. Tulunoglu O, Ayhan H, Olmez A, Bodur H. The effect of cavity disinfectants on microleakage in dentin bonding systems. J Clin Pediatr Dent 1998, 22: 299-305.
26. Vieira Rde S, da Silva IA Jr. Bond strength to primary tooth dentin following disinfection with a chlorhexidine solution: an in vitro study. Pediatr Dent 2003; 25: 49-52.
27. Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res 2000; 34: 498- 501.
28. Emilson CG. Potential efficacy of chlorhexidine against mutans streptococci and human dental caries. J Dent Res 1994; 73: 682- 91.
29. Granath L, Cleaton-Jones P, Fatti LP, Grossman ES. Salivary lactobacilli explain dental caries better than salivary mutants streptococci in 4-5-year-old children. Scand J Dent Res 1994; 102: 319-23.
30. Holmes J. Clinical reversal of root caries using ozone, doubleblind, randomised, controlled 18-month trial. Gerodontology 2003; 20: 106-14.
31. Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006; 114: 349-53.
32. Müller P, Guggenheim B, Schmidlin PR. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 2007; 115: 77-80.
33. Nogales CG, Ferrari PH, Kantorovich EO, Lage-Marques JL. Ozone therapy in medicine and dentistry. J Contemp Dent Pract 2008; 9: 75-84.
34. Hauser-Gerspach I, Pfäffli-Savtchenko V, Dähnhardt JE, Meyer J, Lussi A. Comparison of the immediate effects of gaseous ozone and chlorhexidine gel on bacteria in cavitated carious lesions in children in vivo. Clin Oral Investig 2009; 13: 287-91.
35. Kuştarci A, Sümer Z, Altunbaş D, Koşum S. Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107: 73-9.
36. Noetzel J, Nonhoff J, Bitter K, Wagner J, Neumann K, Kielbassa AM. Efficacy of calcium hydroxide, Er:YAG laser or gaseous ozone against Enterococcus faecalis in root canals. Am J Dent 2009; 22: 14-8.
37. Baysan A, Lynch E. Clinical reversal of root caries using ozone: 6-month results. Am J Dent 2007; 20: 203-8.
38. Baysan A, Beighton D. Assessment of the ozone-mediated killing of bacteria in infected dentine associated with non-cavitated occlusal carious lesions. Caries Res 2007; 41: 337-41