HİLAL ALAN, ÇİĞDEM GÜLER, ÜMİT YOLCU, MAHMUT KOPARAL, İZZETİYE EBRU ÇAKIR, PINAR DEMİR

Biostimulatory Effects of Diode Laser and Ozone on Wound Healing in Rats

Objective: Diode laser and ozone can be used to healing of skin wounds. The aim of this study is to evaluate the biostimulatory effects of diode laser and ozone on the healing of sutured skin wounds in rats. MaterialsandMethods:In this study, 21 adult males Wistar albino rats were used. Three 1-cm-longs, full-thickness incisions were applied to the back of each animal and closed with sutures. Diode laser treatment, irrigation with 0.9% saline solution and ozone therapy was applied to the wounds through 7 days. Animals were euthanized on the 3rd, 7th, and 14th days after treatment, and all wounds were surgically removed for histopathological evaluation. Results: According to histological results, a statistically significant difference was found among the three treatment methods in acute inflammatory reactions on 3rd day (p

Ratlarda Yara İyileşmesinde Ozon ve Diod Lazerin Biyostimülatör Etkisi

Amaç: Diod lazer ve ozon deri yaralarının iyileşmesinde kullanılan yöntemlerdendir. Bu çalışmanın amacı sütüre edilmiş deri yarasında diod lazer ve ozonun biyostimülatör etkisinin değerlendirilmesidir. Gereç ve Yöntemler: Bu çalışmada; 21 erkek Wistar albino rat kullanılmıştır. Hayvanların sırtına üç adet 1 cm uzunluğunda tam kalınlıkta insizyon yapıldı ve sütüre edildi. Yaralara 7 gün diod lazer tedavisi, %0,9 salin solüsyonla irrigasyon ve ozon tedavisi uygulandı. Hayvanlar tedaviden sonra 3., 7., ve 14. günlerde sakrifiye edildi ve histopatolojik değerlendirme için yara bölgesi cerrahi olarak çıkarıldı. Bulgular: Histolojik sonuçlara göre, 3. günde akut enflamatuvar reaksiyon açısından üç tedavi yöntemi arasında istatistiksel olarak anlamlı farklılık vardır (p

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Hodjati H, Rakei S, Johari HG, Geramizedeh B, Sabet B, Zeraatian S. Low-level laser therapy: an experimental design for wound management: a case-controlled study in rabbit model. J Cutan Aesthet Surg 2014; 7: 14-7.

Meirelles GC, Santos JN, Chagas PO, Moura AP, Pinheiro AL. A comparative study of the effects of laser photobiomodulation on the healing of third-degree burns: a histological study in rats. Photomed Laser Surg 2008; 26: 159-66.

Yu H, Yuan L, Xu M, Zhang Z, Duan H. Sphingosine kinase 1 improves cutaneous wound healing in diabetic rats. Injury 2014; 45: 1054-8.

Ihsan FR. Low-level laser therapy accelerates collateral circulation and enhances microcirculation. Photomed Laser Surg 2005; 23: 289-94.

Enwemeka CS, Parker JC, Dowdy DS, Harkness EE, Sanford LE, Woodruff LD. The efficacy of low-power lasers in tissue repair and pain control: a meta-analysis study. Photomed Laser Surg 2004; 22: 323-9.

Zhang F, Lei MP, Oswald TM, Pang Y, Blain B, Cai ZW, et al. The effect of vascular endothelial growth factor on the healing of ischaemic skin wounds. Br J Plast Surg 2003; 56: 334-41.

Bass LS, Treat MR. Laser tissue welding: a comprehensive review of current and future clinical applications. Lasers Surg Med 1995; 17: 315-49.

Moritz A, Schoop U, Goharkhay K, Schauer P, Doertbudak O, Wernisch J, et al. Treatment of periodontal pockets with a diode laser. Lasers Surg Med 1998; 22: 302-11.

Longo L, Evangelista S, Tinacci G, Sesti AG. Effect of diodes-laser silver arsenide-aluminium (Ga-Al-As) 904 nm on healing of experimental wounds. Lasers Surg Med 1987; 7: 444-7.

Melo VA, Anjos DC, Albuquerque Júnior R, Melo DB, Carvalho FU. Effect of low level laser on sutured wound healing in rats. Acta Cir Bras 2011; 26: 129-34.

Valacchi G, Fortino V, Bocci V. The dual action of ozone on the skin. Br J Dermatol 2005; 153: 1096-100.

Martínez-Sánchez G, Al-Dalain SM, Menéndez S, Re L, Giuliani A, Candelario-Jalil E, et al. Therapeutic efficacy of ozone in patients with diabetic foot. Eur J Pharmacol 2005; 523: 151-61.

Bocci V, Borrelli E, Zanardi I, Travagli V. The usefulness of ozone treatment in spinal pain. Drug Des Devel Ther 2015; 9: 2677-85.

. Capon A, Souil E, Gauthier B, Sumian C, Bachelet M, Buys B, et al. Laser assisted skin closure (LASC) by using a 815-nm diode-laser system accelerates and improves wound healing. Lasers Surg Med 2001: 28; 168-75.

Gonzaga Ribeiro MA, Cavalcanti de Albuquerque RL, Santos Barreto AL, Moreno de Oliveira VG, Santos TB, Freitas Dantas CD. Morphological analysis of second-intention wound healing in rats submitted to 16 J/cm 2 lambda 660-nm laser irradiation. Indian J Dent Res 2009; 20: 390.

Oliveira Sampaio SC, de C Monteiro JS, Cangussú MC, Pires Santos GM, dos Santos MA, dos Santos JN, et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 2013; 28: 799-806.

Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M. Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 2005; 31: 334-40.

Mester E, Spiry T, Szende B, Tota JG. Effect of laser rays on wound healing. Am J Surg 1971; 122: 532-5.

Allendorf JD, Bessler M, Huang J, Kayton ML, Laird D, Nowygrod R, et al. Helium-neon laser irradiation at fluences of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. Lasers Surg Med 1997; 20: 340-5.

. Núñez SC, Nogueira GE, Ribeiro MS, Garcez AS, Lage-Marques JL. He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser Doppler flowmetry. Lasers Surg Med 2004; 35: 363-8.

Lins RD, Dantas EM, Lucena KC, Catão MH, Granville-Garcia AF, Carvalho Neto LG. Biostimulation effects of low-power laser in the repair process. An Bras Dermatol 2010; 85: 849-55.

Park JJ, Kang KL. Effect of 980-nm GaAlAs diode laser irradiation on healing of extraction sockets in streptozotocin-induced diabetic rats: a pilot study. Lasers Med Sci 2012; 27: 223-30.

Walsh LJ. The current status of low level laser therapy in dentistry. Part 1. Soft tissue applications. Aust Dent J 1997; 42: 247-54.

Kana JS, Hutschenreiter G, Haina D, Waidelich W. Effect of lowpower density laser radiation on healing of open skin wounds in rats. Arch Surg 1981; 116: 293-6.

Meirelles GC, Santos JN, Chagas PO, Moura AP, Pinheiro AL. A comparative study of the effects of laser photobiomodulation on the healing of third-degree burns: a histological study in rats. Photomed Laser Surg 2008; 26: 159-66.

Mendez TM, Pinheiro AL, Pacheco MT, Nascimento PM, Ramalho LM. Dose and wavelength of laser light have influence on the repair of cutaneous wounds. J Clin Laser Med Surg 2004; 22: 1925.

Gonçalves RV, Novaes RD, Cupertino Mdo C, Moraes B, Leite JP, Peluzio Mdo C, et al. Time-dependent effects of low-level laser therapy on the morphology and oxidative response in the skin wound healing in rats. Lasers Med Sci 2013; 28: 383-90.

Hodjati H, Rakei S, Johari HG, Geramizedeh B, Sabet B, Zeraatian S. Low-level laser therapy: an experimental design for wound management: a case-controlled study in rabbit model. J Cutan Aesthet Surg 2014; 7: 14-7.

Gál P, Mokrý M, Vidinský B, Kilík R, Depta F, Harakalová M, et al. Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats. Lasers Med Sci 2009; 24: 539-47.

. Zhang J, Guan M, Xie C, Luo X, Zhang Q, Xue Y. Increased growth factors play a role in wound healing promoted by noninvasive oxygen-ozone therapy in diabetic patients with foot ulcers. Oxid Med Cell Longev 2014; 273475.

Gupta G, Mansi B. Ozone therapy in periodontics. J Med Life 2012; 5: 59-67.

Valacchi G, Lim Y, Belmonte G, Miracco C, Zanardi I, Bocci V, et al. Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice. Wound Repair Regen 2011; 19: 107-15.

Kim HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci 2009; 24: 368-74.

Koca K, Yurttas Y, Bilgic S, Cayci T, Topal T, Durusu M, et al. Effect of preconditioned hyperbaric oxygen and ozone on ischemiareperfusion induced tourniquet in skeletal bone of rats. J Surg Res 2010; 164: e83-9.

Zhang J, Guan M, Xie C, Luo X, Zhang Q, Xue Y. Increased growth factors play a role in wound healing promoted by noninvasive oxygen-ozone therapy in diabetic patients with foot ulcers. Oxid Med Cell Longev 2014; 273475.