Nidal Amin JARADAT, Abdel Naser ZAID, Fatima HUSSEN, Iyad ALİ

The Effects of Preservation Methods of Grapevine Leaves on Total Phenols, Total Flavonoids and Antioxidant Activity

Preservation methods may affect total phenols, flavonoids contents, and antioxidant capacity of many vegetables and fruits. These effects may cause degradation of antioxidant compounds, formation of new products, or destruction of the active metabolites. This study investigated the effects of different preservation methods, such as canning and freezing, on total flavonoids, total phenols, and antioxidant activity on grapevine leaves, which can be consumed either as medicine or food. Phytochemical screening was performed by using standard analytical methods; antioxidant activity was assayed by using 2,2-diphenyl-1-picryl-hydrazylhydrate reagent method; and total flavonoids and total phenols content were determined by using the rutin reference standard method and by Folin-Ciocalteu’s method, respectively. The phytochemical content of all the studied grapevine leaf extracts were same. Fresh leaf extract showed the highest antioxidant capacity as well as total phenols and flavonoids contents. This was followed by the frozen leaf extract, while the canned leaf extract showed lower antioxidant capacity and reduced phenolic and flavonoids contents. Canning and freezing preservation methods of these leaves had no deleterious effects on total antioxidant capacity as well as total phenols and flavonoid contents. Therefore these methods can be used for preparing nutraceutical, cosmeceutical, and pharmaceutical supplements. However, the preservation of V. vinifera leaves by canning is economically and environmentally favored over freezing. In addition the storing, handling, and maintenance of canned leaves is easier than fresh and frozen leaves.

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Gurib-Fakim A. Medicinal plants: Traditions of yesterday anddrugs of tomorrow. Mol Aspects Med 2006; 27: 1-93.

Boudet AM. Evolution and current status of research inphenolic compounds. Phytochem 2007; 68: 2722-35.

Bombardelli E, Morazzoni P. Vitis vinifera L. Fitoterapia 1995;66: 291-317.

Dani C, Oliboni L, Agostini F, Funchal C, Serafini L, HenriquesJ, et al. Phenolic content of grapevine leaves (Vitis labruscavar. Bordo) and its neuroprotective effect against peroxidedamage. Toxicol In Vitro 2010; 24: 148-53.

Hmamouchi M, Es-Safi N, Essassi E. Oligomeric andpolymeric proanthocyanidins from Moroccan grapewine(Vitis vinifera) leaves. Fitoterapia 1997; 68: 332-7.

Kappor L. Handbook of Ayurvedic Medicinal Plants. CRCPress, Florida. 1990.

Orhan N, Aslan M, Orhan DD, Ergun F, Yesilada E. Invivoassessment of antidiabetic and antioxidant activitiesof grapevine leaves (Vitis vinifera) in diabetic rats. JEthnopharmacol 2006; 108: 280-6.

Mansour R, Ayed L, Hammami S, Mighri Z, Bakhrouf A,Mhenni F. Dyeing properties and antibacterial activities ofthe extracts of Vitis vinifera L. leaves,Tunisia. Tunisian J MedPlants Nat Prod 2011; 6: 126-32.

Kong JM, Chia L-S, Goh NK, Chia TF, Brouillard R. Analysisand biological activities of anthocyanins. Phytochem 2003; 64:923-33.

Yadav R, Agarwala M. Phytochemical analysis of somemedicinal plants. J Phytol 2011; 3: 10-4.

Wang L,Weller CL. Recent advances in extraction ofnutraceuticals from plants. Trends Food Sci Tech 2006; 17:300-12.

Blois MS. Antioxidant determinations by the use of a stablefree radical. Nature 1958; 181: 1199 - 1200.

Evans WC. Trease and Evans’ Pharmacognosy. Elsevier HealthSciences, London. 2009.

Harborne JB. Phytochemical Methods a guide to moderntechniques of plant analysis. Springer Science & BusinessMedia, Germany. 1998.

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, BrunetC, Luyckx M, et al. Phenolic compounds and antioxidantactivities of buckwheat (Fagopyrum esculentum Moench) hullsand flour. J Ethnopharmacol 2000; 72: 35-42.

Batovska DI, Todorova IT, Bankova VS, Parushev SP, AtanassovAI, Hvarleva TD, Djakova GJ, Popov SS. Seasonal variations inthe chemical composition of vine-grape leaf surface. Nat ProdRes 2008; 22: 1231-6.

Liakopoulos G, Nikolopoulos D, Klouvatou A, Vekkos K-A,Manetas Y, Karabourniotis G. The photoprotective role ofepidermal anthocyanins and surface pubescence in youngleaves of grapevine (Vitis vinifera). Ann Bot 2006; 98: 257-65.

Stewart GF, Amerine MA. Introduction to Food Science andTechnology. Elsevier, Netherlands. 2012.

Sharma G, Prakash D, Gupta C. Phytochemicals ofnutraceutical importance: Do they defend against diseases.CAB International, Wallingford. 2014.

Guidoni S, Mannini F, Ferrandino A, Argamante N, Di StefanoR. The effect of grapevine leafroll and rugose wood sanitationon agronomic performance and berry and leaf phenoliccontent of a Nebbiolo clone (Vitis vinifera L.). Am J Enol Vitic1997; 48: 438-42.

Ayala-Zavala JF, Wang SY, Wang CY, Gonzalez-Aguilar GA.Effect of storage temperatures on antioxidant capacity andaroma compounds in strawberry fruit. LWT-Food Sci Technol2004; 37: 687-95.

Felicio J, Santos RdS, Goncalez E. Chemical constituents fromVitis vinifera (Vitaceae). Arquiv Instit Biol 2001; 68: 47-50.

Downey MO, Mazza M, Krstic MP. Development of a stableextract for anthocyanins and flavonols from grape skin. Am JEnol Vitic 2007; 58: 358-64.

Langcake P, Cornford C, Pryce R. Identification of pterostilbeneas a phytoalexin from Vitis vinifera leaves. Phytochem 1979;18: 1025-7.

Eftekhari M, Alizadeh M, Ebrahimi P. Evaluation of the totalphenolics and quercetin content of foliage in mycorrhizalgrape (Vitis vinifera L.) varieties and effect of postharvestdrying on quercetin yield. Ind Crops Prod 2012; 38: 160-5.

Baytop T. Therapy with medicinal plants in turkey (Past andpresent). Istanbul University, Turkey. 1999.

Jaradat NA. Medical plants utilized in Palestinian folkmedicine for treatment of diabetes mellitus and cardiacdiseases. J Al-Aqsa Unv 2005; 9: 1-28.

Czarniecka-Skubina E. Effect of the material form, storage andcooking methods on the quality of Brussels sprouts. Polish JFood Nutr Sci 2002; 11: 75-82.

Lohachoompol V, Srzednicki G, Craske J. The change of totalanthocyanins in blueberries and their antioxidant effect afterdrying and freezing. J Biomed Biotechnol 2004; 5: 248-52

Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, PridhamJB. The relative antioxidant activities of plant-derivedpolyphenolic flavonoids. Free Radic Res 1995; 22: 375-83.