YAVUZ SELİM ÇAKMAK, GÖKHAN ZENGİN, BÜLENT ESKİN, Kevser YILDIRIM, Müge TOPAL, Gözde HATUN AYDIN, Emre ÜNLÜ, Merve BAYDEMİR, Kübra ERTEN

Medicago rigidula (L.) ALL.’nın Antioksidan ve Enzim İnhibisyon Aktiviteleri ve Fenolik Bileşiminin İncelenmesi

Çalışma Medicago rigidula (L.) All. metanol ekstresinin antioksidan kapasitesi, fenolik bileşimi ve enzim inhibisyon aktivitesinin değerlendirilmesi üzerine odaklanmıştır. Antioksidan aktivite 2,2-difenill-1-pikrilhidrazil (DPPH), demir indirgeme antioksidan gücü (FRAP), bakır iyonu indirgeme gücü (CUPRAC) ve total antioksidan kapasite (fosfomolibdat) testini içeren farklı yöntemler kullanılarak değerlendirilmiştir. Aynı zamanda total fenolik ve flavonoit içerikler de belirlenmiştir. Enzim inhibisyon aktiviteleri antikolinesteraz, tirozinaz, alfa-amilaz ve alfa-glukozidaza karşı incelenmiştir. M. rigidula bitkisinin fenolik bileşenlerinin kateşin, sinnamik asit, klorojenik asit, gallik asit ve sirinjik asit bileşikleri olduğu saptanmıştır. Metanol ekstresinin serbest radikal giderici aktivitesi 1 mg/mL konsantrasyonda %20.30’dur. Fosfomolibdat ve FRAP testlerinde sırası ile 167.27 mg AAE/g ve 67.01 mg TE/g bulundu. Total fenolik ve flavonoit içerik sırasıyla 79.61 mg GAE/g ve 27.38 µg QE/g olarak belirlenmiştir. Sonuçlar M. rigidula’nın iyi bir antioksidan ve enzim inhibisyon kapasitesine sahip olduğunu gösterdi. Bu nedenle Medicago rigidula bitkisinin gıda ve ilaç endüstrisinde hammadde olarak kullanılabileceği düşünülmektedir.

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