Development and Validation of Liquid Chromatographic Method with DAD for the Quantification of Natamycin In Dairy Products

In this paper high performance liquid chromatography method was developed for the determination of natamycin in dairy product in Turkish market. Chromatographic separation was achieved on a C8 column (150 mm x 4.6 mm x 5 μm 100 mm) with a mobile phase of methanol : water : acetic acid (12:8:1 v/v/v), at 1ml/min flow rate with DAD detection at 303 nm. Natamycin was analysed simultaneously using HPLC-UV method of ISO 9233+2, 2007( IDF 140-2, 2007). The method was found linear over the concentration ranges of 0.01-0.8 mg/L. Correlation coefficients (r) of the regression equations were greater than 0.999. The LOD and LOQ values for UV and DAD were found 0.321 mg/kg and 0.355 mg/kg; 0.321 mg/kg and 0.388 mg/kg for cheese samples respectively. The relative recoveries obtained for the spiked cheese samples were between 90 and 110 %. Also the interday precision of the method was found as 1.874% and 3.442 % RSD values for UV and DAD dedections. From the results of the present study using HPLC-DAD indicated that it is not reasonable to attribute the determination of natamycin in cheese. Therefore, the difference of the validation parameters were found insignificant for both detections. However, It was succesfully applied to determination of 46 different cheese products and finding dealing with the presence of natamycin in cheese samples were presented.

PDF

___

Chen GQ, Lu FP, and Du LX. Natamycin production bystreptomyces gilvaspaareus base on statistical optimization. JAgric Food Chem 2008; 56: 5057-61.

EFSA 2009 Scientific opinion of EFSA prepared by the panelon food additivies and nutritient sources added to food (ANS)on the use of natamycin (E235) as a food additive. The EFSAJ 2009; 7: 1412-37.

Raab WP In Natamycin (primaricin). Its properties andpossibilities in medicine. Georg Thieme Publ, Stuttgart-NewYork. 1972.

ISO 9233-2, 2007 (IDF 140-2, 2007) Cheese, cheese rind andprocessed cheese- and determination of natamycin content.Part 2 high-performance liquid chromatographic method forcheese, cheese rind and processed cheese.

Sağlam OF. Tuk Gıda Mevzuatı. AB Ofset, Ankara. 1999.

De Ruig WG, Van Oostrorn JJ, Leenheer K. J Assoc Off AnalChem 1987;70: 949-54.

Reps L, Drychowski J, Tomasik K, Niewska W. Natamycin inripening cheeses. Pak J Nutr 2002; 1: 243-7.

Capitan-Vallvey LF, Checa-Moreno R, Navas N, Capitan-Vallvey LF, Checa-Moreno R, Navas N. Rapid ultravioletspectrophotometric and liquid chromatographic methodsfor the determination of natamycin in lactoserum matrix. JAOAC Int 2000; 83: 802-8.

Tuinstra LGMT, Traag WA. Liquid chromatographicdetermination of natamycin in cheese at residue levels. J AssocOff Anal Chem 1982; 65: 820-2.

Rybińska K, Postupolski J, Szczesna M. Determination ofnatamycin residues in ripening cheese by high-performanceliquid chomatography. Rocz Panstw Zakl Hig 1997; 48:173-81.

Fuselli F, Guarino C, La Mantia A, Longo L, Faberi A, Marianella RM. Multi-detection of preservatives in cheese by liquid chromatography - tandem mass spectrometry. J Chromatogr B 2012; 906: 9-18.

Vierikova M, Hrnciarikova E, Lehotay J. Determination of natamycin content in cheese using ultra performance liquid chromatography-mass spectrometry. J Liq Chromatogr Relat Technol 2013; 6: 2933-43.

Guarino C, Fuselli F, La Mantia A and Longo L. Development of an RP-HPLC method for the simultaneous determination of benzoic acid, sorbic acid, natamycin and lysozyme in hard and pasta filata cheese. Food Chem 2011; 127: 1294-9.

Paseiro - Cerrato R, Otero-Pazos P, Rodríguez-Bernaldo de Quirós A, Sendón R, Angulo I. and Paseiro-Losada P. Rapid method to determine natamycin by HPLC-DAD in food samples for compliance with EU Food legislation. Food Control 2013; 33: 262-7.

Thangabalan B, Kumar, PV Analytical method development and validation of Natamycin in eye drop by RP-HPLC. Asian J Pharm Clin Res 2013; 6: 134-5. Bhattaa R.S, Chandasana H, Rathi C, Kumar D, Chhonker YS. and Jain GK. Bioanalytical method development and validation of natamycin in rabbit tears and its application to ocular pharmacokinetic studies. J Pharm Biomed Anal 2011; 54: 1096–110