YUSUF ÇAMLICA, Salih Cüfer BEDİZ, SERAP YALIN

Asetamiprid ve d-Tübokürarin’in Kurbağa Sinir Dokusu Üzerine Etkilerinin İncelenmesi (I: Oksidatif Potansiyel)

Bu çalışmada, asetamiprid ve d-tübokürarin’in Rana ridibunda siyatik siniri üzerine oksidatif stres etkisinin incelenmesi amaçlanmıştır. Bu amaçla kurulan deneylerde, 40 adet kurbağa siyatik sinir preparatı kullanılmıştır. Kurbağalar, spinal hale getirildikten sonra siyatik sinirler izole edilmiştir. Sinirlere, 120 dakika boyunca 1×10-3, 1×10-4, 1×10-5 ve 1×10-6 M olmak üzere dört farklı konsantrasyondaki asetamiprid çözeltileri uygulanmıştır. Bunun yanı sıra siyatik sinir dokuları, 1x10-3 M asetamiprid ile 1x10-2 M d-tübokürarin karışımı, 1x10-5 M asetamiprid ile 1x10-4 M d-tübokürarin karışımı ve 1x10-6 M asetamiprid ile 1x10-5 M d-tübokürarin karışımında 120 dakika bekletilmiştir. Kontrol grubundaki sinir dokuları, 120 dakika süresince Ringer çözeltisinde bekletilmiştir. Tüm deney gruplarında agonist ve antagonist etkileri eşit sayıda denek üzerinde çalışılmıştır (N=5). Kurbağalarda oksidatif stresi değerlendirmek için, katalaz (CAT) ve asetilkolinesteraz (AChE) aktiviteleri ile malondialdehit (MDA) düzeyleri ölçülmüştür. Yapılan biyomarkır analizleri sonucunda, 1x10-3 M asetamiprid’in siyatik sinirde, biyokimyasal parametrelerden CAT ve AChE aktivitesini kontrol grubuna göre önemli biçimde azalttığı gözlenmiştir (P

Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)

The aim of this study is to investigate the oxidative stress effect of acetamiprid and d-tubocurarine on the sciatic nerve of Rana ridibunda by using biochemical methods. For this purpose, 40 frog sciatic nerve preparations were used in the experiments established. Frog sciatic nerves were isolated after making them spinal. Four different concentrations of acetamiprid solution (1×10-3, 1×10-4, 1×10-5 and 1×10-6 M) were applied on nerves for 120 minutes. In addition, the sciatic nerve tissues were maintained for 120 minutes in the mixture of 1x10-3 M acetamiprid and 1x10-2 M d-tubocurarine, the mixture of 1x105 M acetamiprid and 1x10-4 M d-tubocurarine, the mixture of 1x10-6 M acetamiprid and 1x10-5 M d-tubocurarine. Nerve tissues in the control group were maintained in Ringer’s solution for 120 minutes. The agonist and antagonist effects were studied in the experimental group and equal number of subjects (N = 5). Activity of catalase (CAT) and acetylcholinesterase (AChE) as well as malondialdehyde (MDA) levels were measured to evaluate oxidative stress in frogs. The results of the biomarker analysis revealed that the application of 1x10-3 M acetamiprid on sciatic nerve significantly reduced the CAT and AChE activity compared to that of control group (P

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