Sevgin Özlem İŞERİ, Fatma TAVSU, Beyhan SAĞLAM, Feriha ERCAN, Nursal GEDİK, Berrak Ç. YEĞEN

Social isolation stress in the early life reduces the severity of colonic inflammation

Amaç: Hayatın erken dönemindeki stresin, ileri yaştaki akut barsak inflamasyonu ve strese maruz kalma üzerine etkilerini araştırmak. Material Metod: Dişi Sprague–Dawley sıçanlara, doğum sonrası 21. günde, sosyal izolasyon stresi uygulandı. 250 g. ağırlığa ulaşılana dek beklendi. 60 günlük protokolün son 3 gününde, izole edilen, edilmeyen gruplara sudan kaçınma stresi (WAS) 30 dak./gün uygulandı. Sıçanların kalan yarısında ise izole olan, olmayan gruplara, kolona %5'lik asetik asit verilerek kolit oluşturuldu. Son WAS uygulamasından hemen sonra ya da kolit indüksiyonunun 4. gününde sıçanlar dekapite edilerek, TNF-alpha seviyelerinin ölçümü için kan, histolojik değerlendirme, doku myeloperoksidaz (MPO) aktivitesi, nötrofil infiltrasyonunun göstergesi, ölçümü ve glutatyon (GSH) seviyelerinin, antioksidan, ölçümü için kolon dokusu alındı. Bulgular: İzole olan, olmayan guruplarda, WAS ve kolit indüksiyonu serum TNF-alpha, doku MDA seviyelerini, MPO aktivitelerini kontrole göre anlamlı arttırmıştır (p

Hayatın erken döneminde karşılaşılan sosyal izolasyon stresi, koloninflamasyonunun şiddetini azaltır

Objective: To investigate whether the early-life stress interact with a brief stress exposure and acute colonic inflammation in adulthood. Methods: Female Sprague–Dawley rats on the postnatal 21st day were exposed to isolation stress until they reach 250 g. On the last 3 days with or without isolation, water-avoidance-stress (WAS) for 30 min/day or acetic acid(5 %) colitis were performed. After the last WAS or on the 4th day of colitis, rats were decapitated to collect serum for TNF-alpha levels, colonic tissues for histological analysis, myeloperoxidase activity (MPO), evidence of neutrophil infiltration, and glutathione (GSH) levels, a key antioxidant. Results: In the non-isolated and isolated groups, WAS and colitis both elevated the TNF-alpha, MDA levels and MPO activity compared to control (p<0.05-p<0.001). GSH levels were reduced in the non-isolated -WAS or -colitis groups and in the isolated-WAS group (p<0.01-0.001), while GSH level of the isolated-colitis group was not different than control. In the isolated-colitis group, MPO activity and microscopic scores were lower compared to non-isolated group (p<005-0.001). TNF-alpha levels were not different between non-isolated and isolated colitis groups. Conclusion: Post-weaning isolation stress has a protective effect on colonic inflammation and does not exacerbate the colonic response to acute stressors.

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