Azizeh Dizaji, Azizeh Shadi DİZAJI, Reza FATHİ, Abolfazl Sales, Abolfazl Jafari SALES

Iran Tebriz Ostad Alinasab Hastanesi’nin örneklerinden elde edilen Escherichia coli izolatlarında genişletilmiş-spektrum beta-laktamoz (TEM1) geninin araştırılması

Amaç: Antibiyotik direncinin gelişmesi bakteriyel enfeksiyonların kontrolünde önemli bir sorundur. Bu çalışma, antibiyotik duyarlılık profillerini değerlendirmek ve Tebriz’de Ostad Alinasab Hastane’sinin klinik örnekleriden elde edilen E.coli izolatlarında blaTEM geni varlığını değerlendirmek amacıyla planlanmıştır. Gereç ve Yöntemler: E. coli (100 izolatları) geleneksel bakteriyolojik testler ile elde edilmiş ve daha sonra antibiyotic duyarlılık testleri Kirby-Bauer yöntemine göre değerlendirilmiştir. Doğrulayıcı testler ise “Combinde disk” testleri ile gerçekleştirilmiştir. Son olarak blaTEM geni varlığı polymeraz zincir reaksiyon (PCR) tekniği kullanılarak değerlendirilmiştir. Bulgular: Yüz E. coli izolatından %23’nün blaTEM genini içerdiği belirlenmiştir. İzolatlarının %18’inde Seftazidim, % 30’unda sefotaksim direnci saptanmıştır. Izolatların % 46’sında EBSL sentezi belirlenmiş ve bu izolatların % 23’de de blaTEM geninin varlığı saptanmıştır. Sonuç: E. coli izolatlarında saptanan üçüncü kuşak sefalosporinlere karşı yüksek direnç uygunsuz antibiyotik kullanımından kaçınılması gerektiğinin ve tedavi öncesi doğru duyarlılık testlerinin uygulanması gerektiğinin önemin göstermektedir.

Anahtar Kelimeler:

Geniş spektrum beta-laktamaz (GSBL), blaTEM geni, Escherichia coli, Polimeraz zincir reaksiyonu

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