Selim ISBİR, Rıza DOĞAN, Metin DEMİRCİN, İlhan PAŞAOĞLU

APROTININ IMPROVES Ca2+ - Mg2+ / ATPase LEVELS İN ISCHEMIA-REPERFUSION INJURY1'2

Objective: The aim of this study is to determine the effect of aprotinin, on calcium activated and magnesium dependent adenosine 5’ triphosphatase (Ca2+-Mg2+/ATP ase) levels.Methods: Twenty patients who had elective coronary artery bypass grafting (CABG) were randomly divided into two groups (n= 10, in each). Group A patients received full dose aprotinin (20 000 lU/kg as pretreatment followed by 7500 lU/kg for 6 hours) and Group B patients received normal saline as control group. Erythrocyte membrane Ca2+-Mg2+/ATP ase levels were measured.Results: Ca2*-Mg2+ /ATPase levels were protected in aprotinin group, 807.60+32.62 vs 634.28+22.91 (p<0.05) at post-operative 24th hour. (p<0.05).Conclusion: Aprotinin protected the erythocyte membrane Ca2<-Mg2+ ATP/ase from oxidant stress thus it might affect the cell membrane stabilization.Key Words: Aprotinin, Ischemia-Reperfusion injury, Ca2t-Mg2+/ATPase.

PDF

___

Haberland GL, Me Conn RA. A rationale for the therapeutic action of Aprotinin. Fed Proceed 1979; 38: 2760-2767.
Lu ft, Soria C, Commin PL, et at. Hemostasis in patients undergoing extracorporeal circulation; the effect of aprotinin (Trasylol). Thromb Haemost 1991; 66: 633-637.
Diaz PE, Fishbcin MC, Davis MA, Askenazi J, Maroko PR. Effect of kallikrein inhibitor aprotinin on myocardial ischemic injury after coronary occlusion in the dog. Am J Cardiol 1977; 40: 541-549.
Wendel HP, Heller W, Michael J, et al. Lower cardiac troponin T levels in patients
5
C . S e l i m I s b i r , e t a I
undergoing cardiopulmonary bypass and recieving high dose Aprotinin therapy indicate reduction of perioperative myocardial damage. J Thorac Cardiovasc Surg 1995; 109: 1164-1172.
Lucchesi BR, Werns SW, Fan tone JC. The role of the neutrophyl and free radicals in ischemic myocardial injury. J Mol Cell Cardiol 1989; 21: 1241-1251.
Kaneko M, Matsumoto Y, ftayashi fl, Ho bay as hi A, Yamazaki n. Oxygen free radicals and calcium homeostasis in the heart. Mol Cell Biochem 1994; 21: 1241- 1251.
Dick DA, Dick EG, Tusteson D. Inhibition of ATPase in sheep red cell membrane by oxidised glutathione. J Gen Physiol 1969; 54: 123-133.
Murphy E, Aitcon JP, liorres CR, Lieberman M. Calcium elevation in cultured heart cells; its role in cell injury. Am J Physiol 1983; 245: 316-321.
Parr DR, Wimhurst JM, Harris EJ. Calcium induced damage of rat heart mitochondria. Cardiovasc Res 1975; 9: 366-372.
David-Dufilho M, Pernollet MG, LeQuan Sang H, et al. Active Pia+ and Ca2+ transport, Ha+- Ca2+ exchange and intracellular Pia+ and Ca2+ content in young spontaneously
hypertensive rats. J Cardiovasc Pharmacol 1986; 8(suppl 8): 130-135.
Reading HW, Isbir T. Action of lithium on ATPase in transmitter release from rat iris. Biochem Pharmacol 1979; 28: 3471-3477.
Reading HW, Isbir T. The role of cation activated ATPase in transmitter release from rat iris. Q J Exp Psychol 1979; 65: 105-116.
Atkinson A, Gatenby AD, Lowe AG. The determination of inorganic phosphate in biological systems. Biochem Biophys Acta 1973,320: 195-204.
Lowry OH, Rosen burgh HJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951 ; 193: 265- 275.
Haworth RA, Hunter DG, Berkoff HA. Contracture in isolated adult rat heart cells. The role of Ca2+, ATP and compartmentation. Circ res 1981; 49: 1119-1128.
Krause MS, Jacobus EW, Becker LC. Alterations in cardiac sarcoplasmic reticulum calcium transport in the postischemic stunned myocardium. Circ Res 1989; 65: 526-530.
7. Korge P, Campbell KB. Regulation of calcium pump function in back inhibited vesicles by calcium ATP ase ligands. Cardiovasc Res 1995; 29: 512-519.