Ela Nur ŞİMŞEK SEZER, SİNAN AKTAŞ, Fatih DURMAZ, Tuna UYSAL

Pleurotus ostreatus (Jacq.) P. Kumm. Ekstraktı Bazı Apoptotik Genlerin İfadesini Değiştirir

Mantarlar, eski çağlardan beri yiyecek ve tıbbi amaçlarla kullanılmıştır. Özellikle tedavi edici etkisi olan mantarlar birçok araştırma grubunun ilgisini çekmektedir. Aynı zamanda, mantarlardan türetilen aktif bileşiklerin potansiyel olarak yeni antikanser ajanların değerli bir kaynağı olabileceği düşünülmektedir. Bu çalışmanın amacı, Pleurotus ostreatus (Jacq.) P. Kumm'un metanolik ekstraktının apoptozda intrinsik yolda önemli olan bazı gen bölgelerinin ekspresyon seviyeleri üzerindeki etkilerini değerlendirmektir. Bu amaçla mantar örnekleri güneş ışığı görmeden kurutulduktan sonra Soxhlet cihazı ile metanol kullanılarak ekstraktlar hazırlandı. Ekstrelerin sitotoksik etkileri MTT testi ile değerlendirildi. Gerçek zamanlı PCR ile, dört apoptotik gen bölgesinin (Hrk, Bax, Apaf1 ve casp3) ekspresyon seviyelerini değerlendirildi. MTT testinin sonuçları, elde edilen ekstrelerin doza ve zamana bağlı bir şekilde sitotoksik bir etki gösterdiğini göstermiştir. Bununla birlikte P.ostreatus’tan elde edilen metanolik özütlerin apoptotik hücre ölümüyle ilişkili gen bölgelerinin ekspresyon seviyelerinde upregülasyona neden olduğu bulundu. Sonuç olarak, bu çalışma ile, P. ostreatus'un kolorektal kanser üzerinde potansiyel bir terapötik etkiye sahip olduğunu ve bu durumun farklı kanser türleri ve hücre dizileri ile ilgili diğer çalışmalarla uyumlu olduğunu göstermektedir. Bu çalışma, ekstraktaki aktif maddeleri tanımlamaya ve hücre ölümünün moleküler yollarını bulmaya devam edecek gelecekteki çalışmalar için öncü bir çalışmadır.

Anahtar Kelimeler:

cDNA, MTT, RT-PCR, Kavak Mantarı, Türkiye

Pleurotus ostreatus (Jacq.) P. Kumm. Extract Alters the Expression of Some Apoptosis Related Genes

Mushrooms have been used for food and medicinal purposes since ancient times. Especially mushrooms with therapeutic effects attract the attention of many research groups. Besides, it is thought that the active compounds derived from fungi could potentially be a valuable source of new anticancer agents. This study aims to evaluate the effects of methanolic extract of Pleurotus ostreatus (Jacq.) P. Kumm. on the expression levels of some genes important in the intrinsic pathway in apoptosis. For this purpose, after the mushroom samples were dried without sunlight, extracts were prepared via Soxhlet apparatus by using methanol. Cytotoxic effects of the extracts were evaluated with the MTT test. Real-time PCR was performed to evaluate the expression levels of the four apoptotic genes (Hrk, Bax, Apaf1 and casp3). The results of the MTT assay showed that the extracts obtained show a cytotoxic effect in a dose and time-dependent manner. Also, methanolic extracts from P. ostreatus were found to cause upregulation in expression levels of genes which related apoptotic cell death. In conclusion, this study shows that P. ostreatus has a potential therapeutic effect on colorectal cancer and is compatible with other studies of different types of cancer and cell lines. This study is a pioneering study for future studies that will continue to identify the active substances in the extract and find the molecular pathways of cell death.

Keywords:

cDNA, MTT, RT-PCR, Oyster mushroom, Turkey,

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ISSN: 2147-6845
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 2010
Yayıncı: Selçuk Üniversitesi Mantarcılık Uygulama ve Araştırma Merkezi Müdürlüğü