Kerem CANLI, Atakan BENEK, Merve ŞENTURAN, İlgaz AKATA, Ergin Murat ALTUNER

Desarmillaria tabescens’in in vitro Antimikrobiyal Aktivitesi

Mantarların, bazı vitaminlerin ve temel minerallerin kaynağı olan besleyici ve tıbbi gıda maddeleri olduğu bilinmektedir. Ayrıca bazı terapötik maddeler içerirler, bu yüzden yüzlerce yıldır çeşitli sağlık problemlerine karşı kullanılmıştır. Bu çalışmanın amacı Desarmillaria tabescens (Scop.) R.A. Koch & Aime 2017’nin in vitro antimikrobiyal aktivitesini belirlemektir.D. tabescens örnekleri kurutulmuş ve etanol kullanılarak ekstre edilmiştir. D. tabescens etanol ekstraktlarının antimikrobiyal aktivitesi, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) ve Streptococcus pneumoniae (MDR) gibi Gram pozitif ve Gram negatif standart, gıdadan izole edilmiş bakteri suşları, mantar suşları ve klinik izole bazı çoklu ilaca dirençli (MDR) klinik izolat bakteriler kullanılarak disk difüzyon yöntemini ile araştırılmıştır. Sonuç olarak, D. tabescens'in etanol ekstraktlarının, test edilen bazı Gram pozitif ve Gram negatif mikroorganizmaya karşı düşük ila orta antimikrobiyal aktiviteye sahip olduğu gözlenmiştir. D. tabescens'in özellikle K. pneumoniae (MDR) ve S. pneumoniae'ye (MDR) karşı gözlenen antimikrobiyal aktivitesinin dikkat çekici olduğu bulunmuştur.

Anahtar Kelimeler:

Desarmillaria tabescens, antimikrobiyal aktivite, disk difüzyon, çoklu ilaca dirençli bakteriler, MDR

In vitro Antimicrobial Activity of Desarmillaria tabescens

Mushrooms are known to be nutritive and medicinal food stuff, which are good sources of some vitamins and essential minerals. They also contain some therapeutic agents, thus they have been used against several health problems for hundreds of years. The aim of this study is to determine the in vitro antimicrobial activity of Desarmillaria tabescens (Scop.) R.A. Koch & Aime 2017.D. tabescens samples were air dried and extracted by using ethanol. Antimicrobial activity of D. tabescens ethanol extracts were investigated against several Gram positive and Gram negative bacteria strains, fungal strains, which are either standard or isolated from food and some multi drug resistant (MDR) clinical isolate bacteria namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) and Streptococcus pneumoniae (MDR) by using the disk diffusion method. As a result, it was observed that ethanol extracts of D. tabescens has low to medium antimicrobial activity against several Gram positive and Gram negative microorganisms tested. The antimicrobial activity of D. tabescens especially observed against K. pneumoniae (MDR) and S. pneumoniae (MDR) is found to be remarkable.

Keywords:

Desarmillaria tabescens, antimicrobial activity, disk diffusion, multi drug resistant bacteria, MDR,

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ISSN: 2147-6845
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 2010
Yayıncı: Selçuk Üniversitesi Mantarcılık Uygulama ve Araştırma Merkezi Müdürlüğü