BUĞRAHAN EMSEN, Büşranur GÜVEN, ABDULLAH KAYA

Yabani Yenilebilir Bir Mantar Olan Lycoperdon molle Pers.'nin Antioksidan ve Antigenotoksik Potansiyeli

Mevcut çalışmada, yenilebilir önemli mantar türlerinden biri olanLycoperdon molle Pers.'nin kültüre edilmiş insan periferal lenfositleriüzerindeki antioksidan ve antigenotoksik etkilerinin incelenmesiamaçlamıştır. Kurutulmuş taze L. molle'den elde edilen metanol(LMME) ve su (LMSE) ekstraktlarının farklı konsantrasyonları (1-100 mg/L) ile muamele edilen hücrelerdeki toplam oksidan durum(TOD) ve toplam antioksidan kapasite (TAK) değişimleri testedilmiştir. Bu analizlerin sonucunda, LMME ve LMSE'nin 100mg/L'lik konsantrasyonun hücrelerdeki TOD oranını negatif kontrolekıyasla p < 0.05 düzeyinde yükselttiği belirlenmiştir. Her ikiekstraktın 1-25 mg/L konsantrasyonlu uygulamalarının sebep olduğuTAK düzeylerinin negatif kontrol grubu tarafından ortaya çıkarılanTAK oranından yüksek oldukları tespit edilmiştir. İlgili ekstraktlarınhücreler üzerinde genetik hasar oluşturma düzeyleri mikronükleus(MN) ve kromozom aberasyonu (KA) testleri ile belirlenmiştir. Her ikiekstrakt grubunda da 50 ve 100 mg/L konsantrasyonlu uygulamalarharicindeki denemelerin sebep oldukları MN ve KA frekanslarınınnegatif kontrole kıyasla istatistiksel açıdan (p > 0.05) farklı olmadığıtespit edilmiştir.

Antioxidant and Antigenotoxic Potential of Lycoperdon molle Pers., a Wild Edible Mushroom

In the present study, it was aimed to investigate the antioxidant and antigenotoxic effects of Lycoperdon molle Pers., one of the important edible mushroom species, on cultured human peripheral lymphocytes. Total oxidant status (TOS) and total antioxidant capacity (TAC) changes in the cells treated with different concentrations (1-100 mg/L) of methanol (LMME) and water (LMWE) extracts from dried fresh L. molle were tested. In the results of these analyses determined that the concentration of 100 mg/L of LMME and LMWE increased the TOS ratio in the cells at the level of p < 0.05 compared to the negative control. TAC levels caused by the applications of both extracts with 1- 25 mg/L concentration were found to be higher than the TAC ratio, revealed by the negative control group. The levels of genetic damage of the respective extracts on the cells were determined by micronucleus (MN) and chromosome aberration (CA) tests. In both extract groups revealed that MN and CA frequencies which were caused by experiments other than 50 and 100 mg/L concentrations were not statistically (p > 0.05) different from negative control.

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