Van Gölü Havzası’ndan Toplanan Bazı Kavun Genotiplerinde Virüse Karşı Dayanıklılığın Mekanik İnokulasyon ve Moleküler Yöntemlerle Belirlenmesi

Kavun (Cucumis melo L.) Türkiye ‘nin farklı coğrafik bölgelerinde yetiştirilen önemli bir sebze türüdür. Zucchini yellow mosaic virüs (ZYMV) başta olmak üzere cucumber mosaic virüs (CMV) ve watermelon mosaic virüs (WMV) kavunda oluşturduğu yıkıcı hasarlardan dolayı büyük ekonomik kayıplara neden olmaktadır. Bu çalışmada, Van Gölü Havzası’ndan toplanmış olan 22 farklı kavun genotipi ile birlikte 3 dayanıklı genotip ve 2 ticari çeşit bu üç virüse karşı mekanik inokülasyon ve moleküler yöntemlerle taranmıştır. Çalışmada ilk etapta ZYMV virüsü kullanılarak kavun genotiplerine virüs inokulasyonu gerçekleştirilmiş ve genotiplerde morfolojik gözlemler yapılarak 0-5 skalasına göre hastalık şiddeti belirlenmiştir. Çalışmanın ikinci aşamasında bu genotiplerde 3 adet SCAR ve 4 adet CAPS olmak üzere 7 adet primer ile tarama yapılarak ZYMV, CMV ve WMV’ ye karşı dayanıklılık ile ilgili belirteçlerin varlığı tespit edilmeye çalışılmıştır. Şiddetli ZYMV izolatına karşı mekanik olarak test edilen genotiplerin hiçbirinin virüse tolerant, dayanıklı ya da immun olmadığı saptanmıştır. Kavun genotiplerinde görülen hastalık şiddetinin %8.80-95.00 arasında değiştiği belirlenmiştir. SCOPE14541 primerinde 21 genotipte bant tespit edilirken, Creb-2 dayanıklılık genine bağlı olarak geliştirilen SCAPB051046 primerinde 25 genotipten yalnızca U6, U13 ve YYU6 genotiplerinde bant varlığı belirlenmiştir. ZYMV ve WMV’ ye karşı dayanıklılık genlerine bağlı olarak geliştirilmiş olan VirSq-F19 primeri YYU47 genotipi dışında tüm genotiplerde bant vermiştir. Zym dayanıklılık geni ile ilişkili 4 CAPS primerinden sadece ikisinden sonuç elde edilmiştir.

Determination of Virus Resistance in Some Melon Accession Collected from Van Lake Basin by Mechanic Inoculation and Molecular Methods

Melon (Cucumis melo L.) is an important vegetable crop grown in different geographical regions of Turkey. Zucchini yellow mosaic virus (ZYMV), cucumber mosaic virus (CMV) and watermelon mosaic virus (WMV) cause major economic losses because of their destructive effects on melon. In this study, 22 different melon genotypes collected from the Lake Van Basin, three resistant genotypes and two commercial varieties were screened against these three viruses by mechanical inoculation and molecular methods. In the first step, virus inoculation was performed on melon genotypes using ZYMV virus, and disease severity was determined according to 0-5 scale by performing morphological observations in the genotypes. In second stage of the study the presence of markers related to resistance genes against to ZYMV, CMV and WMV were determined by screening with 7 primers including 3 SCAR primers and 4 CAPS primers in these genotypes. None of the genotypes tested mechanically against the severe ZYMV isolate were found to be tolerant, resistant or immune to the virus. It was determined that the disease severity was ranged between 8.80-95.00% in melon genotypes. While bands were obtained from SCOPE14541 primer in 21 genotypes, only U6, U13 and YYU6 genotypes from 25 genotypes gave bands with SCAPB051046 primer that was developed based on the Creb-2 resistance gene. VirSq-F19 primer that was developed based on the resistance genes to ZYMV and WMV gave bands in all genotypes excluding YYU47. Only two of the 4 CAPS primers associated with the Zym resistance gene produced band.

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