Türkiye'de Bir Deniz Akvaryumunda Humpback Red Snapper (Lutjanus gibbus Foorsskal, 1775) ve Golden Trevally'de (Gnathanodon speciosus Foorsskal, 1775) Vibriozisin Teşhisi
Bu çalışma, Türkiye'de bir deniz akvaryumunda bulunan humpback red snapper (Lutjanus gibbus) ve golden trevally (Gnathanodon speciosus) balıklarının ölüm nedenlerinin belirlenmesi amacıyla yapılmıştır. Hasta balıklarda, üst ve alt çenelerde yaygın hemoraji, yüzeysel ve ülseratif deri lezyonları, kuyruk yüzgecinde erime ve solungaçların solgun olduğu gözlemlendi. İç bakıda, periton boşluğunda berrak sıvı birikmesi ve solgun karaciğer tespit edildi. İzole edilen bakteriler morfolojik, fizyolojik, biyokimyasal özelliklerine ve API 20E sonuçlarına göre Vibrio harveyi olarak tanımlandı. Bu bakteriyel tanımlamalar 16S rRNA gen dizisi analizi ile de doğrulanmıştır. İzolatların sülfametoksazol/trimetoprim, florfenikol ve flumequin'e duyarlı oldukları ve eritromisin, kanamisin ve ampisiline dirençli oldukları belirlendi. Vibrio harveyi ile enfekte olmuş balıklarda genellikle solungaç filamentlerinde epitel hiperplazisi, ülseratif deri lezyonları, hepatik hücrelerde, böbrek tübüllerinde ve dalağın parankim hücrelerinde dejenerasyon veya nekroz, böbrekte multifokal melanomakrofaj merkezleri ve dalakta hemopoietik dokuda boşalma tespit edildi.
Diagnosis of Vibriosis in Humpback Red Snapper (Lutjanus gibbus Foorsskal, 1775) and Golden Trevally (Gnathanodon speciosus Foorsskal, 1775) in a Marine Aquarium, Turkey
This study was carried out to determine the reason of mortalities inhumpback red snapper (Lutjanus gibbus) and golden trevally(Gnathanodon speciosus) kept in a marine aquarium in Turkey.Moribund fish showed diffuse haemorrhages on upper and lower jaws,superficial and ulcerative skin lesions, tail rot and pale gills.Internally, clear fluid in peritoneal cavity and pale liver wereobserved. The isolated bacteria were identified as Vibrio harveyiaccording to their morphological, physiological, biochemicalcharacteristics and API 20E results. These bacterial identificationswere also confirmed with 16S rRNA gene sequence analysis. Theisolates were determined to be sensitive to sulphamethoxazole/trimethoprim, florphenicol and flumequine and were resistant toerythromycin, kanamycin and ampicillin. Vibrio harveyi infected fishgenerally showed epithelial hyperplasia in the gill filaments,ulcerative skin lesions, degeneration or necrosis of the hepatic cells,kidney tubules and parenchyma cells of the spleen, multifocalmelanomacrophage centres in the kidney and reduced hemopoietictissue in the spleen.
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