Mn, Cd, Fe ve Mg Metallerinin Saccharomyces cerevisiae Mayasında Antioksidan Enzim Aktiviteleri Üzerine Etkisi

Metal iyonları, kalıcı etkilerinden dolayı hem canlı sistemler hem deçevre sağlığı yönünden önem taşımakta olup, belirli bir sınırı aşıncada son derece toksik etki gösterirler. Özellikle ağır metaller bu konudaen riskli grubu oluşturur. Bu çalışmada Saccharomyces cerevisiae 'da,mangan (Mn), kadmiyum (Cd), demir (Fe) ve magnezyumun (Mg)antioksidan enzimler üzerindeki etkisinin ortaya konmasıamaçlanmıştır. Deney materyali olan S. cerevisiae FMC16, YEDPbesiyerinde çoğaltılmış ve geliştirilmiştir. Uygulama grupları için;Mn, Cd, Fe ve Mg metallerinin her biri son derişimi 100 mL’de 1 mgolacak şekilde kültür ortamına ilave edilmiş ve uygulama yapılan(deneysel grup) ve yapılmayan (kontrol grubu) mayalarda süperoksitdismutaz (SOD), glutatyon S-transferaz (GST) ve glutatyon redüktaz(GSH-Rd)enzimaktivitelerispektrofometrikyöntemlerlebelirlenmiştir. Sonuç olarak; kontrol grubuna göre tüm deneyselgruplarda SOD aktivitesinin arttığı ve bu artışın istatiksel açıdanönemli olduğu ( p

The Effects of Mn, Mg, Cd, Fe Metals on The Avtivitiy of Antioxidant Enzymes in Saccharomyces cerevisiae

Metal ions, despite being important for both live systems and environmental health due to their permanent effects, and may become extremely toxic effect if they exceed a certain limit. Especially heavy metals are considered as the most risky group in this regard. In this study it is intended to reveal the effect of manganese (Mn), cadmium (Cd), iron (Fe) and magnesium (Mg) on antioxidant enzymes in Saccharomyces cerevisiae. S. cerevisiae FMC16, the strain used in this experiments, was proliferated and developed on YEDP medium. For application groups; Mn, Cd, Fe and Mg metals were separately added to the culture medium at the final concentration of 1 mg at 100 mL. Superoxide dismutase (SOD), glutathione S-transferase (GST) and glutathione reductase (GSH-Rd) enzyme activities were determined spectrophotometrically in the treated (experimental group) and non-treated (control group) yeasts. As a result; it was observed that SOD activity was increased and the increase was statistically significant (p

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