Tıbbi Bitki Lysimachia nummularia L.'nın Boğum Eksplantlarından In Vitro Mikroçoğaltımı

Lysimachia nummularia L. Avrupa ve Asya'da çeşitli hastalıklarıntedavisinde geleneksel olarak kullanılan önemli bir bitkidir. İçerdiğideğerli biyoaktif bileşiklerden dolayı farmasötik alanda oldukçaönemli bir yere sahiptir. Bu çalışmada, L. nummularia'nın dokukültürü teknikleri ile üretimi araştırılmıştır. L. nummularia'nınboğum eksplantları farklı konsantrasyonlarda 6-Benzilaminopurin(BAP)'i tek olarak veya İndol-3-Bütirik Asit (IBA) ilekombinasyonunu içeren Murashige ve Skoog (MS) besin ortamındakültüre alınmıştır. Genel olarak her iki hormon denemelerindeyüksek sürgün rejenerasyon yüzdeleri elde edilmiştir. Maksimumeksplant başına sürgün sayısı (12.27 adet) 1.6 mg L-1 BAP içeren MSbesin ortamında, ardından 0.8 mg L-1 BAP + 0.1 mg L-1 IBA içeren MSbesin ortamında (11 adet) elde edilmiştir. En düşük sürgün sayısı heriki hormon uygulamasında da 0.05 mg L-1 BAP içeren MS besiortamında tespit edilmiştir. Kültür ortamında BAPkonsantrasyonunun artması ile sürgün sayısı artış göstermiştir. Enuzun sürgünler 0.1 mg L-1 BAP eklenmiş MS ortamında (4.6 cm) ve0.1 mg L-1 BAP + 0.1 mg L-1 IBA eklenmiş MS ortamında (4.52 cm)kaydedilmiştir. En kısa sürgün uzunlukları 1.6 mg L-1 BAP eklenmişMS ortamında tespit edilmiştir. Kültür ortamlarındaki rejeneresürgünler yoğun kök oluşturdukları için ayrıca köklendirme çalışmasıyapılmamıştır. Köklü bitkiler başarılı şekilde akvaryum ortamınaalıştırılmıştır. Sonuç olarak L. nummularia'nın doku kültürüteknikleri ile üretimi için en iyi hormon 1.6 mg L-1 BAP olarak tespitedilmiştir. Bu çalışma, L. nummularia'nın doku kültürü ile çokluüretimine imkan sağlayarak, bu bitkiden değerli bitkiselmetabolitlerin yüksek miktarlarda elde edilmesine yardımcı olabilir.Böylece gıda ve farmasötik sektörlere katkı sunulabilir.

In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.

Lysimachia nummularia L. is an important plant that has traditionally been used in the treatment of various diseases in Europe and Asia. Due to the valuable bioactive compounds content, this plant has a very important place in the pharmaceutical field. In this study, the propagation of L. nummularia by tissue culture techniques was investigated. The nodal explants of L. nummularia were cultured in Murashige and Skoog (MS) nutrient media containing 6- benzylaminopurine (BAP) alone or in combination with indole-3-butyric acid (IBA) at different concentrations. Generally, high rates of shoot regeneration were obtained in both hormone trials. The maximum number of shoots per explant (12.27) was obtained in the MS nutrient medium containing 1.6 mg L-1 BAP, followed by MS medium containing 0.8 mg L-1 BAP + 0.1 mg L-1 IBA (11). The lowest number of shoots was determined in MS medium containing 0.05 mg L-1 BAP in both hormone applications. The number of shoots increased with increasing BAP concentration in the culture medium. The longest shoots were recorded in MS medium supplemented with 0.1 mg L-1 BAP (4.6 cm) and 0.1 mg L-1 BAP + 0.1 mg L-1 IBA (4.52 cm). The shortest shoot lengths were determined in MS medium supplemented with 1.6 mg L-1 BAP. Since the regenerated shoots in the culture medium sustaine intensive roots, rooting experiments have not been carried out. The rooted plants were successfully acclimatized to the aquarium environment. As a result, the best hormone for the propagation of L. nummularia by tissue culture techniques was 1.6 mg L-1 BAP. This work may help to obtain high amounts of this valuable herbal metabolite by allowing multiple production of L. nummularia with tissue culture. Thus, it can be contributed to the food and pharmaceutical sectors.

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