EMİNE MİNE SOYLU, SONER SOYLU, Merve KARA, Şener KURT

Sebzelerde Sorun Olan Önemli Bitki Fungal Hastalık Etmenlerine Karşı Vermikomposttan İzole Edilen Mikrobiyomların in vitro Antagonistik Etkilerinin Belirlenmesi

Bu çalışmada, solucan gübresi olarak bilinen vermikompostlardan eldeedilen bakteriyel mikrobiyomların sebzelerde sorun olan önemli yaprakve toprak kökenli fungal hastalık etmenlerinden Sclerotinia sclerotiorum,Macrophomina phaseolina, Botrytis cinerea, Verticilium dahliae üzerineolan antagonistik etkinlikleri in vitro koşullarda araştırılmıştır. Yapılanizolasyonlar sonucunda toplam 69 adet aday bakteriyel izolat eldeedilmiştir. Elde edilen bakteriyel izolatlar arasında 28 bakteriyel izolat(toplam izolatın %49.12) in vitro ikili kültür testlemelerinde fungaletmenlerden S. sclerotiorum’un gelişimini %1.72-75.43, M. phaseolina’nıngelişimini %1.67-65.83, B. cinerea gelişimini %3.44-57.18, V. dahliaegelişimini ise %2.28-58.74 gibi değişen oranlarda engellemişlerdir.Antagonist potansiyele sahip bakteriyel izolatların çoğunluğununBacillus spp.’e ait olduğu belirlenmiştir. İzolatlar arasında bazı Bacillusspp.’ye ait izolatlar fungal hastalık etmeni S. sclerotiorum hifleriüzerinde önemli morfolojik değişikliklere neden olmuştur. Antagonistbakterilerin in vitro antagonizm etkinliği, fungus inokulasyonu öncesi öninkübasyon süresine bağlı olarak artış göstermiştir. Bacillus spp. aitizolatlar yüksek antagonistik özelliklerinden dolayı organik vesürdürülebilir tarımı teşvik etmek için toprak kökenli hastalıklarlamücadelede kimyasallara alternatif etkili biyo-kontrol ajanlar olarakkullanılabilir.

Determinations of in vitro Antagonistic Effects of Microbiomes Isolated from Vermicompost Against Major Plant Fungal Disease Agents of Vegetables

In this study, in vitro antagonistic potentials of bacterial microbiomes, obtained from earthworm fertiliser, vermicompost, were investigated on inhibitions of mycelial growth of major foliar and soilborne fungal disease agents Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, Verticilium dahliae. Total of 69 putative bacterial biocontrol agent (BCA) isolates were obtained from commercial vermicompost lots. Among them, 28 bacterial isolates (49.12% of total isolates) were inhibited mycelial growth of S. sclerotiorum by 1.72- 75.43%, M. phaseolina by 1.67-65.83%, B. cinerea by 3.44-57.18%, V dahliae by 2.28-58.74%, respectively. Majorities of bacterial isolates were identified as Bacillus spp. Certain isolates of Bacillus spp. have caused noticeable morphological changes on mycelia of S. sclerotiorum. Antagonistic potentials of bacterial isolates were found to increase by pre-incubation time prior the fungal inoculation. Due to high antagonistic properties, efficient isolates of Bacillus spp. may be used as biocontrol agent against soilborne diseases as an alternative to pesticides to promote organic and sustainable agriculture.

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