Selim DEMİR, İbrahim TURAN, Elif AYAZOĞLU DEMİR, Ufuk ÖZGEN

Onosma armeniacum Ekstraktının Çeşitli Kanser Hücrelerindeki Sitotoksik Etkisinin Değerlendirilmesi

Bu çalışmanın amacı O. armeniacum kök ekstraktının antioksidan özellikleri ile normal bir fibroblast hücre hattına kıyasla insan karaciğer (HepG2), akciğer (A549) ve kolon (WiDr) kanser hücre hatlarındaki sitotoksik etkisini araştırmaktır. Ekstraktın antioksidan özellikleri kolorimetrik yöntemler kullanılarak belirlenmiştir. Ekstraktın sitotoksik etkisi MTT yöntemi kullanılarak araştırılmıştır. Ekstraktın toplam fenolik içerik ve indirgeyici güç değerleri, g örnek başına sırasıyla 26.1±0.4 mg gallik asit eşdeğeri ve 52.6±1.1 mg troloks eşdeğeri olarak hesaplanmıştır. Ekstrakt, çalışılan tüm kanser hücre serilerinde, özellikle HepG2 (3.6 kat), seçici sitotoksik etki göstermiştir. Çalışmanın sonuçları, kabul edilebilir antioksidan ve sitotoksik etkilere sahip olduğu saptanan O. armeniacum'un daha kapsamlı çalışmalarla incelenmesi gerektiğini ortaya koymaktadır.

Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells

The aim of this study was to investigate the antioxidant properties ofthe root extract of Onosma armeniacum and its cytotoxic effect againsthuman liver (HepG2), lung (A549) and colon (WiDr) cancer cell linescompared with a normal fibroblast cell line. Antioxidant properties ofthe extract was determined using the colorimetric methods. Thecytotoxic effect of the extract were investigated using MTT assay. Thetotal phenolic content and reducing power values of extract were26.1±0.4 mg gallic acid equivalent and 52.6±1.1 mg trolox equivalentper g sample, respectively. The extract exhibited selective cytotoxiceffect in all studied cancer cell lines, especially HepG2 (3.6-fold). Theresults of this study reveal that O. armeniacum, which has been foundto have acceptable antioxidant and cytotoxic effects, should beexamined with more comprehensive studies.

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