Kahramanmaraş İlindeki Paliurus spina-christi Mill. Bitkisinin Antimikrobiyal ve Antioksidan Aktivitelerinin Araştırılması

Çalışmada, Kahramanmaraş ilinde bulunan Paliurus spina–christi Mill. (Karaçalı) bitkisinin meyve ve yaprak kısımlarından elde edilen su ve etanol ekstrelerinin antioksidan ve antimikrobiyal aktiviteleri incelenmiştir. Ekstrelerin antioksidan aktiviteleri, kuprik iyonu (Cu+2) indirgeme (CUPRAC), 1,1-Difenil 2-pikril hidrazil (DPPH) ve 2,2-azinobis (3-etilbenzo-tiyazolin-6-sülfonik asit) (ABTS) yöntemleri ile belirlenmiştir. Sonuçlar, BHT (Bütil hidroksitoluen) ve Troloks standart maddeleriyle karşılaştırılarak değerlendirilmiştir. Bitki ekstrelerinin antimikrobiyal aktiviteleri ise agar kuyu difüzyon yöntemi kullanılarak belirlenmiştir. CUPRAC yöntemine göre, bitki ekstrelerinin 800 μg/ml konsantrasyondaki antioksidan aktivitelerinin, standart antioksidan maddelere göre daha düşük olduğu tespit edilmiştir. Aynı konsantrasyonda, bitkinin yaprak kısmının etanol ekstresinin DPPH radikali giderme aktivitesinin Trolokstan daha yüksek olduğu tespit edilmiştir. ABTS yönteminde ise, bitkinin meyve kısmının radikal giderme aktivitesinin yüksek ve standart antioksidanlara yakın olduğu tespit edilmiştir. Antimikrobiyal çalışmalardan elde edilen verilere göre Pasteurella multicoda ve Bacillus cereus bakterileri dışında tüm mikroorganizmalar üzerinde meyve ve yaprak kısmından elde edilen etanol ekstrelerinin en yüksek zon çapına sahip olduğu, bitkinin meyve kısmının etanol ekstresi ise Pseudomonas aeruginosa, Klebsiella pneumoniae ve Candida albicans’da daha etkili olduğu tespit edilmiştir.

Investigation of Antimicrobial and Antioxidant Activities of Paliurus spina-christi Mill. in Kahramanmaras, Turkey

In this study, the antioxidant and antimicrobial activities of the water and ethanol extracts obtained from the fruit and leaves of Paliurus spina-christi Mill, from Kahramanmaras city were investigated. The antioxidant activities of the extracts were determined using the cupric ion (Cu+2) reducing antioxidant capacity (CUPRAC), 1,1-diphenyl-2- picryl-hydrazyl-hydrate (DPPH), and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. The results were evaluated by comparing them with those of butylated hydroxytoluene (BHT) and Trolox, as standard substances. On the other hand, the antimicrobial activities of the plant extracts were identified using the agar well diffusion method. According to the CUPRAC method, the antioxidant activities of the plant extracts at a concentration of 800 μg/mL were found to be lower than those of the standard antioxidant substances. At the same concentration, the DPPH radical scavenging activity of the ethanolic extract of the leaves of the plant was found to be higher than that of Trolox. In the ABTS method, the radical scavenging activity of the fruit of the plant was found to be high and close to those of the standard antioxidants. According to the data obtained from antimicrobial studies, ethanol extracts obtained from fruit and leaf parts on all microorganisms except Pasteurella multicoda and Bacillus cereus bacteria have the highest zone diameter. The ethanol extract of fruit of the plant was found to be more effective in Pseudomonas aeruginosa, Klebsiella pneumoniae and Candida albicans.

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