Ceyda CEYHAN, Mustafa KÜSEK, Zeynep Hümeyra ARDIÇ

Kahramanmaraş Biber Alanlarından İzole Edilen Toprak Bakterilerinin Phytophthora capsici’ye Karşı Etkisinin Belirlenmesi

Kahramanmaraş ilindeki biber ekim alanlarındaki biber bitkilerinden daha iyi gelişmiş sağlıklı bitkiler seçilerek kök bölgelerinden köklerle birlikte 36 toprak örneği alınmıştır. Bu toprak örneklerinden bakteri izolasyonu yapılarak elde edilen bakteri kolonilerinden morfolojik karakter farklılıkları göz önüne alınarak 713 izolat elde edilmiştir. Engelleme zonlarının yarıçapları ölçülmek suretiyle en etkili izolatın ZHA17 olduğu belirlenmiştir. Phytophthora capsici’ye karşı bu bakterilerden engelleme zonu oluşturan 15 izolat seçilerek saksı çalışmasında kullanılmıştır. Saksı çalışmasında ZHA57, ZHA88, ZHA178, ZHA212, ZHA215, ZHA287 ve ZHA579 izolatları kök boğazı yanıklığı hastalığının gelişimini engellemiştir. Biolog Gen III tanılama sistemine göre izolatlar Bacillus pumilus, Bacillus subtilis ss subtilis, Mycobacterium confluentis, Mycobacterium immunogenum, Paenibacillus castaneae, Pseudomonas fluorescens, Pseudomonas viridilivida ve Tsukamurella paurometabola bakteri türleri olduğu belirlenmiştir.

Effect of Rhizospheres Bacteria Isolated from Kahramanmaraş Pepper Fields Against Phytophthora capsici

For the study, 36 soil samples with roots parts were taken from rootzone of better grown healthy pepper plants from the pepper fields ofKahramanmaras province. Bacterial isolations were made fromcollected soil samples. Overall, 713 isolates were obtained from thebacteria colonies based on the morphological characteristicsdifferences. By measuring the radius of inhibition zone, ZHA17 wasdetermined to be most effective isolate. Fifteen isolates were selectedfrom these bacteria in such a way to establish a zone to inhibit thegrowth of Phytophthora capsici and used in pot experiments undercontrolled conditions. The isolates were identified as Bacillus pumilus,B. subtilis ss subtilis, Mycobacterium confluentis, M. immunogenum,Paenibacillus castaneae, Pseudomonas fluorescens, P. viridilivida andTsukamurella paurometabola bacteria species according to BiologGEN III identification system.

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