In vitro Koşullarda Ceratophyllum demersum L.’un Krom (III) Akümülasyonunun Araştırılması
Bu çalışma, farklı konsantrasyon (0-12 mg/L) ve sürelerde (1, 3 ve 5gün) kroma (Cr) maruz bırakılan Ceratophyllum dermersum L. 'unağır metal akümülasyon seviyelerini ve Cr toksisitesinin taze vekuru ağırlıklar, fotosentetik pigmentasyon (klorofil a, klorofil b,toplam klorofil ve karotenoid), protein içeriği ve lipidperoksidasyonu üzerine etkilerini sunmaktadır. Cr akümülasyonu,metal konsantrasyonu ve uygulama süresinin artışı ile artışgöstermiştir. En fazla Cr akümülasyon değeri 12 mg/L Cruygulamasında ve 5 gün sonunda tespit edilmiştir. Crtoksisitesinden kaynaklı fotosenteik pigment ve proteiniçeriklerinde önemli azalışlar kaydedilmiştir. Kontrol gruplarıylakarşılaştırıldığında, minimum fotosentetik pigment seviyeleri 5 günsonra 12 mg/L kromda belirlenmiştir. Protein içeriğindekimaksimum azalma %35.84 olarak tespit edilmiştir. Malondialdehit(MDA) içeriği, lipid peroksidasyonunun bir ürünü olarakölçülmüştür. Konsantrasyon ve uygulama süresindeki artış iledoğru orantılı olarak MDA içeriği de artmıştır (p
Investigation of Chromium (III) Accumulation of Ceratophyllum demersum L. under In vitro Conditions
This study presents heavy metal accumulation levels of Ceratophyllum dermersum L. exposed to chromium (Cr) at different concentrations (0-12 mg/L) and durations (1, 3 and 5 days) and effects of Cr toxicity on fresh and dry weights, photosynthetic pigmentation (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), protein contents and lipid peroxidation. Cr accumulation increased with increasing metal concentration and exposure time. The highest Cr accumulation value was determined at 12 mg/L Cr application and after 5 days. Significant reductions in photosynaptic pigment and protein contents from Cr toxicity were noted. Compared with the control groups, minimum photosynthetic pigment levels were determined at 12 mg/L Cr after 5 days. The maximum reduction in the protein content was determined to be 35.84%. Malondialdehyde (MDA) content was measured as a product of lipid peroxidation. MDA content has also increased in direct proportion to the increase in concentration and application time (p
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