Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Fritillaria imperialis needs 2-3 years to regenerate from seeds, whichmakes its production quite challenging. In this study, effects of threeconcentrations of 6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15mg/L) and three different amounts (0.0, 3.0 and 3.5 g/L) of activatedcarbon (AC) on the growth of F. imperialis plantlets in vitro weredetermined. The auxins were at similar concentration (0.01 mg/L ofNAA and 0.01 mg/L of IAA) for all treatments. It has been found thatthe size and number of bulblets, as well as root and shoot numbers inregenerated plantlets were increased significantly (p

6-benzilaminopurin ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi Üzerindeki Etkileri

Fritillaria imperialis türü bitkiler, tohumlarını yenilemek için iki yıldan fazla bir süreye ihtiyacı duymakta ve bu durum üretimlerini zorlaştırmaktadır. Bu çalışmada, üç farklı derişimdeki 6- Benzilaminopurinin (BAP) (0.00, 0.10 ve 0.15 mg/L) ve üç farklı miktardaki (0.0, 3.0 ve 3.5 g/L) aktif karbonun (AC) F. imperialis bitkilerinin büyümesine etkisi in vitro olarak incelenmiştir. Tüm muamele grupları için benzer konsantrasyonda oksin (0.01 mg/L NAA ve 0.01 mg/L IAA) kullanılmıştır. 0.10 mg/L BAP ilave edilmiş kültür ortamlarına 3.5 g/L AC eklenmesinin bitkilerin yumru çap uzunluğu ve sayısının yanında kök ve sürgün sayılarını istatiksel olarak anlamlı bir şekilde (p

PDF

___

Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endangered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity and Conservation, 14(5): 1059-1069.

Anagnostakis S 1974. Haploid plants from anthers of tobacco - Enhancement with charcoal. Planta, 155(3): 281-283.

Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousia tryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland, Australia. Biodiversity Conservation, 11(8): 1469-1477.

Cheng Y, Ma R, Jiao Y, Qiao N, Li T 2013. Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L). African Journal of Botany, 88: 306-309

Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue and Organ Culture, 40(3): 231-235.

Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular and Developmental Biology-Plant, 42(5): 455-460.

Eshaghi M, Shiran B, Fallahi H, Ravash R, Deri B 2018. Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. Genomics, https//doi.org/ 10.10161/jygeno.

Fridborg G, Eriksson T 1975. Effects of activated charcoal on growth and morphogenesis in cell cultures. Physiologia Plantarum, 34(4): 306-308.

Fridborg G, Pedersen M, Landstrom, LE, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum, 43(2): 104- 106.

George EF, Hall MA, Klerk GJD 2008. Plant tissue culture procedure – background. In: George EF, Hall MA, Klerk GJD (Eds.), Plant Propagation by Tissue Culture, Springer, Dordrecht, pp. 1-28.

Li S, Li P, Lin G, Chan S, Ho Y 2000. Simultaneous determination of seven major isosteroidal alkaloids in bulb of Fritillaria by gas chromatography. Chromatographia, 873(2): 221-228.

Li X, Gao W, Jiang Q, Huang L, Liu C 2011. Study on the morphology crystalline structure, and thermal properties of Fritillaria ussuriensis Maxim starch acetates with different degrees of substitution. Starch-Starke, 63(1): 24-31.

Nayanakantha N, Singh B, Kumar A 2010. Improved culture medium for micropropagation of Aloe vera L. Tropical Agricultural Research and Extension, 30: 87-93.

Nhut D, Van Le, Fukai S, Tanaka M, Van K 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation,33(1):59-65. Olah R 2017. The use of activated charcoal in grapevine tissue culture. Grapevine research, 56: 161-171.

Rønsted N, Law S, Thornton H, Fay M, Chase M 2005. Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classification of Fritillaria. Molecular Phylogenetic and Evolution, 35: 509-527

Saeed N, Cömertpay S 2017. Activated charcoal improves growth of F. imperialis propagated by indirect organogenesis. Columella/ Journal of Agricultural Environmental Sciences, 4(1): 59-64.

Sipayung P, Matanri J, Br Lafau M, Sri Sulastri Y, Ginting B, Sihombing D, Pandiangan M, Giawa T 2018. The effect of AC dose and benzyl amino purine concentration on the growth of orchid plantlets in Murashige and Skoog media in vitro. In: International Conference on Agribusiness, IOP Conference Series: Earth and Environmental

Science, 20-21 October, Nusa Dua, Bali, pp. 012025 Staikkidou I, Selby C, Hanks G 2000. Stimulation of in vitro bulblet growth in Galanthus species with sucrose and activated charcoal. Acta Horticulturae, 725: 421-426.

Steinits B, Yahel H 1982. In vitro propagation of Narcissus tazetta. Horticulture Science, 17(3): 333- 334.

Sun C, Wang D 1991. Fritillaria (Fritillary): In vitro culture and the regeneration of plants. In: Bajaj YPS (Eds.), Medicinal and Aromatic Plants lll. Biotechnology in Agriculture and Forestry, Springer, Berlin, pp. 258-269.

Wang S, Gao W, Chen H, Xiao P 2005. New starches from Fritillaria species medicinal plants. Carbohydrate Polymerases, 61(1): 111-114.