Bazı Ruta L. Ekstraktlarının Biyoaktif Madde İçerikleri ve Radikal Kovucu Aktiviteleri

Ruta L. geleneksel tıbbi bitki olarak yaygın kullanılan, Rutaceaefamilyasının cinslerinden biridir. Bu çalışmada, yabani Rutachalepensis L. (toprak üstü kısmı) ve Ruta montana L. (yaprak-çiçekve gövde kısımları) türlerinin metanol ve etanol ekstraktlarınınantioksidan aktiviteleri incelenmiştir. En yüksek ekstraktsiyonverimini R. montana (çiçek-yaprak) metanol ekstraktı sağlamıştır (%15.11, 151.12 ± 0.78 mg / g). En düşük verim ise R. montana (gövde)etanol ekstraktında (% 2.67, 26.66 ± 0.50 mg / g) tespit edilmiştir. R.chalepensis metanol ve etanol ekstraktlarının kuru maddeiçerisindeki toplam flavonoid madde içeriğinin, R. montana metanolçiçek-yaprak ekstraktı hariç diğer ekstraktlarından daha yüksekolduğu gözlenmiştir. Toplam flavonoid ve fenolik madde içeriği en azR. montana gövde kısmının etanol ekstraktlarından elde edilmiştir.Ayrıca ekstraktların 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)ve 2, 2′-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic acid (ABTS) gibiradikal süpürücü aktiviteleri araştırılmış ve çözücü bazındadeğerlendirildiğinde R. chalepensis ekstraktlarının daha yüksekaktivite sergilediği gözlenmiştir. Sonuç olarak, bu çalışmada eldeedilen verilerden yararlanılarak Ruta ekstraktlarının doğal birantioksidan kaynağı olabilirliliği tartışılmıştır.

Total Bioactive Contents and Radical Scavenging Activities of Some Ruta L. Extracts

Ruta L. is a genus of the Rutaceae family, mostly used as a traditional medicinal plant. In this study, the antioxidant activities of methanolic and ethanolic extracts of wild Ruta chalepensis L. (above ground plant) and Ruta montana L. (leaves-flowers, stems) were assessed. The results showed that methanol extract of flower-leave of Ruta montana provided the highest yield of extraction (15.11%, 151.12±0.78 mg/g) whereas the lowest yield was obtained in ethanol extract of stems of Ruta montana (2.67%, 26.66±0.50 mg/g). Total flavonoid content in dry weight of methanol and ethanol extracts of Ruta chalepensis was found higher than other extracts except methanol leaves-flowers extract of Ruta montana. It was determinated that ethanol extracts of Ruta montana stem sustained less total flavonoid and phenolic content. Also, radical scavenging activities such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2, 2′-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic acid (ABTS) of extracts was investigated, and according to the solvent, it was revealed that extracts of Ruta chalepensis exhibited high activity. As a result, it has been discussed that Ruta can be used as a natural antioxidant by taking advantage of these data.

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