Günnur ONAK PULAT, Gülşah SUNAL, Ozan KARAMAN

Silikat Katkılı Esnek β-TCP/PLA Kemik Greftleri ile İnsan Mezenkimal Kök Hücrelerinin Gelişmiş Osteojenik Farklılaşması

Son yıllarda biyolojik ve mekanik olarak uygun kemik iskeleleri geliştirmek için seramikler, polimerler ve bunların kompozitleri kullanılmaktadır. β-trikalsiyum fosfat（β-TCP), kimyasal bileşimi orijinal kemiğin kimyasal yapısına benzediğinden, mükemmel osteoiletkenlik, osteoindüktif ve iyi biyouyumluluk özellikleri gösteren, kemik dokusu mühendisliğinde yaygın olarak kullanılan bir seramiktir. Burada, iki farklı konsantrasyonda silikat katkı maddesi içeren β-TCP-PLA kompozit yapı iskeleleri tasarladık. Amacımız, değişen konsantrasyonlarda (%0,8 ve %1) silikat katkı maddesinin kemik greftleri üzerine ekilen insan kemik iliği kaynaklı mezenkimal kök hücrelerinin (iMKH'ler) osteojenik farklılaşması üzerindeki etkisini araştırmaktı. β-TCP-PLA bazlı kemik greftlerinin moleküler yapısı, taramalı elektron mikroskobu (TEM) kullanılarak değerlendirildi. Ayrıca greftlerin çekme mukavemeti de değerlendirildi. Karakterizasyon sonuçları, iskelelerin gözenekli ve esnek yapılara sahip olduğunu göstermiştir. iMKH'lerin osteojenik farklılaşması, alkalin fosfataz (ALP) aktivitesi ve DNA içeriği ölçümleri ile değerlendirildi. β-TCP-PLA greftleri ile karşılaştırıldığında, %0,8 ve %1 silikat katkılı bu tasarlanmış sentetik esnek kemik greftleri, hMSC'lerin çoğalmasını ve osteojenik farklılaşmasını önemli ölçüde desteklemiştir. Ayrıca, %0.8 silikat katkılı β-TCP-PLA greftleri, keskin bir şekilde artan ALP aktivitesi gösterdi. Bu çalışmanın sonuçları, silikat katkılı sentetik esnek kemik greftlerinin kemiğin yenilenmesini teşvik etmek için yararlı olabileceğini önermektedir.

Anahtar Kelimeler:

kemik doku mühendisliği, β-TCP/PLA kemik greftleri, silikat, osteogenez

ENHANCED OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY FLEXIBLE β-TCP/PLA BONE GRAFTS WITH SILICATE ADDITIVE

In recent years, ceramics, polymers, and composites have been used to develop biologically and mechanically suitable bone scaffolds. β-tricalcium phosphate（β-TCP) is a widely used bioceramic in bone tissue engineering. It shows excellent osteoconductivity, osteoinductivity, and good biocompatibility properties, as its chemical composition is similar to the original chemical structure of bone. Herein, we designed β-TCP-PLA composite scaffolds containing two different concentrations of silicate additives. We aimed to investigate the effect of silicate-additive with varying concentrations (0.8% and 1%) on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) seeded flexible bone grafts. The morphological structure of β-TCP-PLA-based bone grafts was assessed by scanning electron microscopy (SEM) and the tensile strength of grafts was evaluated. The results showed that scaffolds had porous and flexible structures. hMSCs osteogenic differentiation was evaluated with the alkaline phosphatase (ALP) activity and DNA content measurements. Compared with β-TCP-PLA grafts, these designed synthetic flexible bone grafts with 0.8% and 1% silicate-additive significantly promoted hMSCs proliferation and osteogenic differentiation. Moreover, 0.8% silicate-additive β-TCP-PLA grafts showed increased ALP activity. The outcomes of the present study suggest that synthetic flexible bone grafts with silicate-additive might be useful for encouraging the regeneration of bone.

Keywords:

Bone Tissue Engineering, Β-TCP/PLA Bone Grafts, Silicate, Osteogenesis,

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